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Spectrométrie de masse et hémocultures: un nouvel outil de diagnostic immédiat

Mass spectrometry and blood cultures: a new instant diagnosis tool

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Bio tribune magazine

Résumé

Le retard à l’identification des microorganismes est une barrière à l’établissement d’une antibiothérapie empirique adéquate des bactériémies. La spectrométrie de masse MALDI-TOF-MS permet d’obtenir une identification rapide des microorganismes responsables de bactériémies, moyennant une adaptation de la technique qui a fait ses preuves sur colonies. Les protocoles publiés sont très variés. Ils sont tous fondés sur la concentration et la séparation des microorganismes des hématies et des substances contenues dans le bouillon d’hémoculture, pouvant dégrader la qualité des spectres. La grande majorité des études publiées à ce jour ont donné des taux d’identification supérieurs à 80 %, dans un délai de 20 minutes à une heure, à partir du signal de la détection de la positivité.

La spectrométrie MALDI-TOF-MS, de loin la plus rapide des techniques d’identification, représente donc une avancée incontestable dans l’amélioration de la prise en charge du malade bactériémique.

Abstract

Delay in identification of microorganisms hamper the prompt initiation of adequate empirical antibiotic therapy of bacteremia. MALDI-TOF-MS allows identification of microorganisms directly from colonies within minutes. Identification of microorganisms responsible for bacteremia can be made directly from blood culture bottles. Many different protocols have been published, all based on the separation of microorganisms from erythrocytes and blood culture broth that alter the quality of spectra. Correct identifications were obtained for most of the procedures used in at least 80% of cases, within 20 to 60 min once the blood culture is detected positive.

MALDI-TOF-MS, by far the fastest identification technique, offers a significant breakthrough in the management of bacteremia.

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Authors and Affiliations

  1. Laboratoire de Microbiologie, APHP, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015, Paris, France

    A. Ferroni, S. Suarez, E. Bille, J. -L. Beretti & X. Nassif

  2. Andromas SAS, 156 rue de Vaugirard, Paris, France

    B. Dauphin

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  1. A. Ferroni
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  2. S. Suarez
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  3. E. Bille
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  4. B. Dauphin
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  5. J. -L. Beretti
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  6. X. Nassif
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Correspondence to A. Ferroni.

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Ferroni, A., Suarez, S., Bille, E. et al. Spectrométrie de masse et hémocultures: un nouvel outil de diagnostic immédiat. Bio trib. mag. 40, 31–36 (2011). https://doi.org/10.1007/s11834-011-0058-4

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  • DOI: https://doi.org/10.1007/s11834-011-0058-4

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