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Biochemical components of wild relatives of chickpea confer resistance to pod borer, Helicoverpa armigera

Arthropod-Plant Interactions volume 14, pages 623–639 (2020)Cite this article

Abstract

Efforts are being made to develop chickpea varieties with resistance to the pod borer, Helicoverpa armigera for reducing pesticide use and minimizing the extent of losses due to this pest. However, only low to moderate levels of resistance have been observed in the cultivated chickpea to this polyphagous pest. Hence, it is important to explore wild relatives as resistance sources to develop insect-resistant cultivars. Therefore, we studied different biochemical components that confer resistance to H. armigera in a diverse array of wild relatives of chickpea. Accessions belonging to wild relatives of chickpea exhibited high levels of resistance to H. armigera as compared to cultivated chickpea genotypes in terms of lower larval survival, pupation and adult emergence, decreased larval and pupal weights, prolonged larval and pupal developmental periods and reduced fecundity of the H. armigera when reared on artificial diet impregnated with lyophilized leaf powders. Amounts of proteins and phenols in different accessions of chickpea wild relatives were significantly and negatively correlated with larval weight, pupation and adult emergence. Phenols showed a negative correlation with pupal weight and fecundity, but positive correlation with pupal period. Total soluble sugars showed a negative correlation with larval period, but positive correlation with pupation and pupal weight, while tannins showed a positive correlation with larval weight, pupation and adult emergence. The flavonoid compounds such as chlorogenic acid, ferulic acid, naringin, 3,4-dihydroxy flavones, quercetin, naringenin, genistein, biochanin-A and formononetin that were identified through HPLC fingerprints, exhibited negative effects on survival and development of H. armigera reared on artificial diet impregnated with lyophilized leaf powders. The wild relatives with diverse mechanisms of resistance conferred by different biochemical components can be used as sources of resistance in chickpea breeding programs to develop cultivars with durable resistance to H. armigera for sustainable crop production.

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Acknowledgements

We thank the entomology staff at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India for their support in carrying out the experiments. The financial support provided by Department of Science and Technology (DST), New Delhi, India, under the INSPIRE Fellowship scheme to Dr Siva Kumar is gratefully acknowledged.

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Authors and Affiliations

  1. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana, 502324, India

    Siva Kumar Golla, Hari Chand Sharma, Suraj Prashad Mishra & Jagdish Jaba

  2. Acharya N.G, Ranga Agricultural University, Guntur, Andhra Pradesh, 522509, India

    Siva Kumar Golla & P. Rajasekhar

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  1. Siva Kumar Golla
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  2. Hari Chand Sharma
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Correspondence to Siva Kumar Golla.

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Golla, S.K., Sharma, H.C., Rajasekhar, P. et al. Biochemical components of wild relatives of chickpea confer resistance to pod borer, Helicoverpa armigera. Arthropod-Plant Interactions 14, 623–639 (2020). https://doi.org/10.1007/s11829-020-09768-3

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Keywords

  • Chickpea
  • Wild relatives
  • Helicoverpa armigera
  • Antibiosis
  • Biochemical mechanism of resistance

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