Abstract
The invasive Brown Marmorated Stink Bug (BMSB), Halyomorpha halys Stål, has dispersed widely throughout North America and Europe, negatively impacting agro-ecosystems and urban areas. This species is phytophagous and highly gregarious at all developmental stages. Therefore, it is important to determine how the congeners react to plant defenses induced by first infestation. Lipoxygenase activity was found to be enhanced in faba bean (Vicia faba L.) leaves by BMSB feeding or its salivary compounds. We analyzed BMSB feeding behavior by comparison with our previously published EPG waveform library for that pest, and identified some EPG variables associated with test probes, stylets pathway, and sustained ingestion. We demonstrated that, on elicited plants, BMSB probes were delayed, with sustained ingestion events being shorter. Moreover, significant changes in salivary gland proteins involved in plant allelochemical detoxification were detected when BMSB was exposed to plant defenses. Our results confirmed that this polyphagous invasive Heteroptera has the ability to detect plant defenses and to adapt its feeding strategies in consequence.
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LS, LP, MLF, and FF conceived and designed research. LS, LP, and MS conducted experiments and analyzed data. LS wrote the manuscript. All authors read and approved the manuscript.
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11829_2019_9718_MOESM1_ESM.xlsm
Supplementary Table S1: Calculated electropenetrography variables related to Halyomorpha halys feeding according to the plant treatment. N = number of replicates taken into account for each variable. SEM = Standard Error of the Mean. NS = Non Significant difference, according to Kruskal–Wallis method. NA = Non Applicable. Supplementary material 1 (XLSM 15 kb)
11829_2019_9718_MOESM2_ESM.xlsm
Supplementary Table S2: Differentially expressed proteins in salivary glands of Halyomorpha halys exposed to induced plant defenses. Supplementary material 2 (XLSM 95 kb)
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Serteyn, L., Ponnet, L., Saive, M. et al. Changes of feeding behavior and salivary proteome of Brown Marmorated Stink Bug when exposed to insect-induced plant defenses. Arthropod-Plant Interactions 14, 101–112 (2020). https://doi.org/10.1007/s11829-019-09718-8
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DOI: https://doi.org/10.1007/s11829-019-09718-8