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Effects of three novel resistant black raspberry selections on Amphorophora agathonica feeding behavior and performance

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Abstract

Host plant resistance is a practical and cost-effective approach for growers to manage insect pests. Recently, three new sources of resistance in black raspberry (Rubus occidentalis; selections ORUS 3778-1, ORUS 3817-1, and ORUS 4109-1) against the large raspberry aphid, Amphorophora agathonica, were identified. We studied stages of host plant acceptance: host plant attraction, parturition (deposition of nymphs), nymph survival, and adult feeding behavior (using the electrical penetration graph [EPG]) to identify the location of the plant resistance. Aphids were more attracted to the susceptible cultivar ‘Munger’ (control) than the resistant selections. Parturition occurred on the resistant selections, but fewer nymphs were deposited on resistant lines relative to the susceptible control. Nymphs survived only an average of 3.3–3.6 days on resistant selections, whereas 94 % were still alive after 11 days on ‘Munger.’ There were differences in feeding behavior between the susceptible control and the resistant selections, but no differences between the three resistant selections. The tissues responsible for resistance appear to be the mesophyll and phloem sieve elements. Aphids had a reduced probability of salivation into the phloem sieve elements of resistant selections, and only one aphid each on ORUS 3778-1 and ORUS 4109-1 successfully ingested from the phloem. Because feeding behavior of A. agathonica did not differ between resistant selections, independent confirmation that resistance is conferred by unique genes should be obtained before pyramiding these sources together.

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Acknowledgments

Thanks to N. Mosier for propagation of the resistant black raspberries from tissue culture, North American Plants for donation of ‘Munger’ plants, B. Mackey for statistical advice, and C. Fieland and J. Mindolovich for assisting in behavioral assays. The authors declare no conflict of interest. Funding was provided by a US Department of Agriculture Specialty Crops Research Initiative grant 2009-51181-06022, and CRIS 5358-22000-037-00D.

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Authors and Affiliations

  1. University of California Cooperative Extension, P.O. Box 697, Orland, CA, 95963, USA

    D. Lightle

  2. British Columbia Blueberry Council, C/O Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, 6947 Hwy #7, P.O. Box 1000, Agassiz, BC, V0M 1A0, Canada

    M. Dossett

  3. Citrus Research and Education Center, University of Florida, 700 Experiment Station Dr., Lake Alfred, FL, 33850, USA

    T. Ebert

  4. USDA ARS Horticultural Crops Research Unit, 3420 NW Orchard Ave, Corvallis, OR, 97330, USA

    C. E. Finn, R. R. Martin & J. C. Lee

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  1. D. Lightle
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  2. M. Dossett
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  3. T. Ebert
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  4. C. E. Finn
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  5. R. R. Martin
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  6. J. C. Lee
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Correspondence to D. Lightle.

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Lightle, D., Dossett, M., Ebert, T. et al. Effects of three novel resistant black raspberry selections on Amphorophora agathonica feeding behavior and performance. Arthropod-Plant Interactions 9, 487–496 (2015). https://doi.org/10.1007/s11829-015-9390-z

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  • DOI: https://doi.org/10.1007/s11829-015-9390-z

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