EPG-Calc: a PHP-based script to calculate electrical penetration graph (EPG) parameters

Abstract

Electrical penetration graph (EPG) technique is a powerful tool to investigate the hidden feeding behavior of piercing–sucking insects allowing to link recorded EPG waveforms to stylet penetration and complex behaviors related to feeding activities occurring within plant tissue. Calculating the numerous EPG parameters necessary to unravel the complex insect–plant interactions is very time consuming, and few tools have been developed to automate it. EPG-Calc is a rich internet application intended to fill this gap, providing a fast and user-friendly web-based interface that uses analysis files from dedicated software (STYLET+) or database-compatible CSV text files containing waveform codes and cumulative time as input, and produces output files in database-compatible CSV text or Microsoft Excel® XLS format that are directly usable by different statistical analysis softwares. EPG-Calc greatly reduces the time needed for EPG parameters calculation and allows to calculate more than 100 different parameters based on standardized definitions and calculus methods in such a way that avoid confusion between all kinds of definitions and calculations by individual authors.

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References

  1. Ameline A, Couty A, Martoub M, Sourice S, Giordanengo P (2010) Modification of Macrosiphum euphorbiae colonization behaviour and reproduction on potato plant treated by mineral oil. Entomol Exp Appl 135:77–84

    Article  Google Scholar 

  2. Backus EA, Habibi J, Yan FM, Ellersieck M (2005) Stylet penetration by adult Homalodisca coagulata on grape: electrical penetration graph waveform characterization, tissue correlation, and possible implications for transmission of Xylella fastidiosa. Ann Entomol Soc Am 98:787–813

    Article  Google Scholar 

  3. Backus EA, Cline AR, Ellerseick MR, Serrano MS (2007) Lygus hesperus (Hemiptera: Miridae) feeding on cotton: New methods and parameters for analysis of nonsequential electrical penetration graph data. Ann Entomol Soc Am 100:296–310

    Article  Google Scholar 

  4. Bonani JP, Fereres A, Garzo E, Miranda MP, Appezzato-Da-Gloria B, Lopes JRS (2010) Characterization of electrical penetration graphs of the Asian citrus psyllid, Diaphorina citri, in sweet orange seedlings. Entomol Exp Appl 134:35–49

    Article  Google Scholar 

  5. Boquel S, Ameline A, Giordanengo P (2011) Assessing aphids potato virus Y-transmission efficiency: a new approach. J Virol Methods 178:63–67

    CAS  PubMed  Article  Google Scholar 

  6. Brunissen L, Cherqui A, Pelletier Y, Vincent C, Giordanengo P (2009) Host-plant mediated interactions between two aphid species. Entomol Exp Appl (in press)

  7. Brunissen L, Vincent C, Le Roux V, Giordanengo P (2010) Effects of systemic potato response to wounding and jasmonate on the aphid Macrosiphum euphorbiae (Sternorryncha: Aphididae). J Appl Entomol 134:562–571

    CAS  Google Scholar 

  8. Calatayud PA, Rahbé Y, Tjallingii WF, Tertuliano M, Leru B (1994) Electrically recorded feeding-behavior of Cassava mealybug on host and nonhost plants. Entomol Exp Appl 72:219–232

    Article  Google Scholar 

  9. Carpane P, Wayadande A, Backus E, Dolezal W, Fletcher J (2011) Characterization and correlation of new electrical penetration graph waveforms for the Corn leafhopper (Hemiptera: Cicadellidae). Ann Entomol Soc Am 104:515–525

    Article  Google Scholar 

  10. Civolani S, Leis M, Grandi G, Garzo E, Pasqualini E, Musacchi S, Chicca M, Castaldelli G, Rossi R, Freddy Tjallingii W (2011) Stylet penetration of Cacopsylla pyri; an electrical penetration graph (EPG) study. J Insect Physiol 57:1407–1419

    CAS  PubMed  Article  Google Scholar 

  11. Dugravot S, Brunissen L, Letocart E, Tjallingii WF, Vincent C, Giordanengo P, Cherqui A (2007) Local and systemic responses induced by aphids in Solanum tuberosum plants. Entomol Exp Appl 123:271–277

    Article  Google Scholar 

  12. Dugravot S, Backus EA, Reardon BJ, Miller TA (2008) Correlations of cibarial muscle activities of Homalodisca spp. sharpshooters (Hemiptera: Cicadellidae) with EPG ingestion waveform and excretion. J Insect Physiol 54:1467–1478

    CAS  PubMed  Article  Google Scholar 

  13. Fereres A, Moreno A (2009) Behavioural aspects influencing plant virus transmission by homopteran insects. Virus Res 141:158–168

    CAS  PubMed  Article  Google Scholar 

  14. Gabrys B, Pawluk M (1999) Acceptability of different species of Brassicaceae as hosts for the cabbage aphid. Entomol Exp Appl 91:105–109

    Article  Google Scholar 

  15. He YP, Chen L, Chen JM, Zhang JF, Chen LZ, Shen JL, Zhu YC (2011) Electrical penetration graph evidence that pymetrozine toxicity to the rice brown planthopper is by inhibition of phloem feeding. Pest Manag Sci 67:483–491

    CAS  PubMed  Article  Google Scholar 

  16. Huang F, Tjallingii WF, Zhang PJ, Zhang JM, Lu YB, Lin JT (2012) EPG waveform characteristics of solenopsis mealybug stylet penetration on cotton. Entomol Exp Appl 143:47–54

    Article  Google Scholar 

  17. Jacobson AL, Kennedy GG (2013) Effect of cyantraniliprole on feeding behavior and virus transmission of Frankliniella fusca and Frankliniella occidentalis (Thysanoptera: Thripidae) on Capsicum annuum. Crop Prot 54:251–258

    CAS  Article  Google Scholar 

  18. Janssen JAM, Tjallingii WF, Vanlenteren JC (1989) Electrical recording and ultrastructure of stylet penetration by the greenhouse-whitefly. Entomol Exp Appl 52:69–81

    Article  Google Scholar 

  19. Jiang YX, Lei H, Collar JL, Martin B, Muniz M, Fereres A (1999) Probing and feeding behavior of two distinct biotypes of Bemisia tabaci (Homoptera: Aleyrodidae) on tomato plants. J Econ Entomol 92:357–366

    Google Scholar 

  20. Jin S, Chen ZM, Backus EA, Sun XL, Xiao B (2012) Characterization of EPG waveforms for the tea green leafhopper, Empoasca vitis Gothe (Hemiptera: Cicadellidae), on tea plants and their correlation with stylet activities. J Insect Physiol 58:1235–1244

    CAS  PubMed  Article  Google Scholar 

  21. Johnson DD, Walker GP (1999) Intracellular punctures by the adult whitefly Bemisia argentifolii on DC and AC electronic feeding monitors. Entomol Exp Appl 92:257–270

    Article  Google Scholar 

  22. Kimmins FM, Bosque-Perez NA (1996) Electrical penetration graphs from Cicadulina spp and the inoculation of a persistent virus into maize. Entomol Exp Appl 80:46–49

    Article  Google Scholar 

  23. Le Roux V, Dugravot S, Campan E, Dubois F, Vincent C, Giordanengo P (2008) Wild Solanum resistance to aphids: antixenosis or antibiosis? J Econ Entomol 101:584–591

    PubMed  Article  Google Scholar 

  24. Lett JM, Granier M, Grondin M, Turpin P, Molinaro F, Chiroleu F, Peterschmitt M, Reynaud B (2001) Electrical penetration graphs from Cicadulina mbila on maize, the fine structure of its stylet pathways and consequences for virus transmission efficiency. Entomol Exp Appl 101:93–109

    Article  Google Scholar 

  25. McLean DL, Kinsey MG (1984) The precibarial valve and its role in the feeding behavior of the pea aphid, Acyrthosiphon pisum. Bull Entomol Soc Am 30:26–31

    Google Scholar 

  26. Pointeau S, Ameline A, Laurans F, Salle A, Rahbé Y, Bankhead-Dronnet S, Lieutier F (2012) Exceptional plant penetration and feeding upon cortical parenchyma cells by the woolly poplar aphid. J Insect Physiol 58:857–866

    CAS  PubMed  Article  Google Scholar 

  27. Pompon J, Quiring D, Giordanengo P, Pelletier Y (2010a) Characterization of Solanum chomatophilum resistance to two aphid potato pests, Macrosiphum euphorbiae (Thomas) and Myzus persicae (Sulzer). Crop Prot 29:891–897

    Article  Google Scholar 

  28. Pompon J, Quiring D, Giordanengo P, Pelletier Y (2010b) Role of host-plant selection in resistance of wild Solanum species to Macrosiphum euphorbiae and Myzus persicae. Entomol Exp Appl 137:73–85

    Article  Google Scholar 

  29. Powell G (2005) Intracellular salivation is the aphid activity associated with inoculation of non-persistently transmitted viruses. J Gen Virol 86:469–472

    CAS  PubMed  Article  Google Scholar 

  30. Prado E, Tjallingii WF (1994) Aphid activities during sieve element punctures. Entomol Exp Appl 72:157–165

    Article  Google Scholar 

  31. Prado E, Tjallingii WF (2007) Behavioral evidence for local reduction of aphid-induced resistance. J Insect Sci 7:48

    PubMed Central  Article  Google Scholar 

  32. Sarria E, Cid M, Garzo E, Fereres A (2009) Excel workbook for automatic parameter calculation of EPG data. Comput Electron Agric 67:35–42

    Article  Google Scholar 

  33. Spiller NJ (1990) An ultrastructural study of the stylet pathway of the brown planthopper Nilaparvata lugens. Entomol Exp Appl 54:191–193

    Article  Google Scholar 

  34. Tjallingii WF (1978) Electronic recording of penetration behaviour by aphids. Entomol Exp Appl 24:721–730

    Article  Google Scholar 

  35. Tjallingii WF (1985a) Electrical nature of recorded signals during stylet penetration by aphids. Entomol Exp Appl 38:177–186

    Article  Google Scholar 

  36. Tjallingii WF (1985b) Membrane-potentials as an indication for plant-cell penetration by aphid stylets. Entomol Exp Appl 38:187–193

    Article  Google Scholar 

  37. Tjallingii WF, Hogen Esch T (1993) Fine structure of aphid stylet routes in plant tissues in correlation with EPG signals. Physiol Entomol 18:317–328

    Article  Google Scholar 

  38. Tjallingii FW (2013) List EPG variables. http://www.epgsystems.eu/downloads.php

  39. Ullman DE, McLean DL (1988) The probing behavior of the summer-form pear psylla. Entomol Exp Appl 47:115–125

    Article  Google Scholar 

  40. Van Giessen WA, Jackson DM (1998) Rapid analysis of electronically monitored homopteran feeding behavior. Ann Entomol Soc Am 91:145–154

    Google Scholar 

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Acknowledgments

We are grateful to Dr Sébastien Dugravot and Dr Julien Saguez for their help in testing the script. Many thanks to Dr Freddy Tjallingii for his invaluable help in calculation methods.

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Correspondence to Philippe Giordanengo.

Additional information

EPG-Calc can be accessed online at http://www2.sophia.inra.fr/ID/epg.php.

Handling Editor: Henryk Czosnek.

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Giordanengo, P. EPG-Calc: a PHP-based script to calculate electrical penetration graph (EPG) parameters. Arthropod-Plant Interactions 8, 163–169 (2014). https://doi.org/10.1007/s11829-014-9298-z

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Keywords

  • Hemiptera
  • Aphid
  • Feeding behavior
  • Insect–plant interaction
  • Automated software