Skip to main content
SpringerLink
Log in

Polyelectrolyte microcapsules as the systems for delivery of biologically active substances

  • Experimental Studies
  • Published:
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

Based on the method of the layer-by-layer (LbL) adsorption of oppositely charged polyelectrolytes, sodium alginate (Alg) and poly-L-lysine (PLL), novel biodegradable microcapsules have been prepared for delivery of biological active substances (BAS). Porous spherical CaCO3 microparticles were used as templates. The template cores were coated with several layers of oppositely charged polyelectrolytes forming shell on the core surface. The core-shell microparticles were converted into hollow microcapsules by means of core dissolution with EDTA. Mild conditions for microcapsules preparation allow to perform incorporation of various biomolecules maintaining their bioactivity. Biocompatibility and biodegradability of the polyelectrolytes give a possibility to use the microcapsules as the target delivery systems. Chymotrypsin entrapped into the microcapsules was used as a model enzyme. The immobilized enzyme retained about 86% of the activity compared to a native chymotrypsin. The resultant microcapsules were stable in acidic medium and could be easily decomposed by trypsin treatment in slightly alkaline medium. Chymotrypsin was shown to be active after its release from the microcapsules decomposed by the trypsin treatment. Thus, the microcapsules prepared by the LbL technique can be used for the development of new type of BAS delivery systems in humans and animals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Benita, S., Micro encapsulation, Drugs, and the Pharmaceutical Science, Marcel Dekker, New York, 1996.

    Google Scholar 

  2. Allen, T.M. and Cullis, P.R., Science, 2004, vol. 303, pp. 1818–1822.

    Article  CAS  Google Scholar 

  3. Yoshioka, T., Hashida, M., Muranishi, S., and Sezaki, H., Int. J. Rharm., 1981, vol. 8, pp. 131–141.

    Article  Google Scholar 

  4. Gombotz, W.R., and Wee, S.F., Adv. Drug Del. Rev., 1998, vol. 31, pp. 267–285.

    Article  CAS  Google Scholar 

  5. Soppimath, K.S., Aminabhavi, T.M., Kulkarni, A.R., and Rudzinski, WE., J. Control. Rel., 2001, vol. 70, pp. 1–20.

    Article  CAS  Google Scholar 

  6. Kreuter, J., in Specialized Drug Delivery System, Tyle, P., Ed., New York: Marcel Dekker, Inc., pp. 257–266.

  7. Barsukov, L.I., Sorosovskii Obrazovatel’nyi Zhurnal, 1998, vol. 10, pp. 2–9.

    Google Scholar 

  8. Donath, E., Sukhorukov, G.B., Caruso, F., Davis, S.A., and Möhwald, H., Angew. Chem. Int. Ed., 1998, vol. 37, pp. 2201–2205.

    Article  Google Scholar 

  9. Mayya, S., Schoeler, B., and Caruso, F., Adv. Funct. Mater., 2003, vol. 13, pp. 183–188.

    Article  CAS  Google Scholar 

  10. Shenoy, D.B., Antipov, A.A., Sukhorukov, G.B., and Mohwald, H., Biomacromolecules, 2003, vol. 4, pp. 265–272.

    Article  CAS  Google Scholar 

  11. Qiu, X.P., Leporatti, S., Donath, E., and Mohwald, H., Langmuir, 2001, vol. 17, pp. 5375–5380.

    Article  CAS  Google Scholar 

  12. Fisher, K.A., Huddersman, K.D., and Taylor, M.J., Chem. Eur. J., 2003, vol. 9, pp. 5873–5878.

    Article  CAS  Google Scholar 

  13. Li, Z.Z., Wen, LX, Shao, L., and Chen, J.F., J. Control Release, 2004, vol. 98, pp. 245–254.

    Article  CAS  Google Scholar 

  14. Kim, H.W., Knowles, J.C., and Kim, H.E., Biomaterials, 2004, vol. 25, pp. 1279–1287.

    Article  CAS  Google Scholar 

  15. Kortesuo, P., Ahola, M., Kangas, M., Leino, T., Laakso, S., Vuorilehto, L., Yli-Urpo, A., Kiesvaara, J., and Marvola, M., J. Control Release, 2001, vol. 76, pp. 227–238.

    Article  CAS  Google Scholar 

  16. Otsuka, M., Tokumitsu, K., and Matsuda, Y., J. Control Release, 2000, vol. 67, pp. 369–384.

    Article  CAS  Google Scholar 

  17. Charnay, C., Begu, S., Tourne-Peteilh, C., Nicole, L., Lerner, D.A., and Devoisselle, J.M., Eur J. Pharm. Biopharm., 2004, vol. 57, pp. 533–540.

    Article  CAS  Google Scholar 

  18. Volodkin, D.V., Larionova, N.I., and Sukhorukov, G.B., Biomacromolecules, 2004, vol. 5, pp. 1962–1972.

    Article  CAS  Google Scholar 

  19. Sukhorukov, G.B., Donath, E., Moy, S., and Susha, A.S., Micro encapsulation, 2000, vol. 17, no. 2, pp. 177–185.

    Google Scholar 

  20. Berth, G., Voigt, A., Dautzenberg, H., Donath, E., and Mohwald, H., Biomacromolecules, 2002, vol. 3, pp. 579–590.

    Article  CAS  Google Scholar 

  21. Balabushevitch, N.G., Tiorina, O.P., Volodkin, D.V., Larionova, N.I., and Sukhorukov, G.B., Biomacromolecules, 2003, vol. 4, pp. 1191–1197.

    Article  Google Scholar 

  22. Worthington Enzyme Manual, Worthington Biochemical Corporation, 2003, USA, pp. 97–105.

  23. Sukhorukov, G.B., Donath, E., Davis, S., Lichtenfeld, H., Caruso, F., Popov, V.I., and Mohwald, H., Polym. Adv. Technol., 1998, vol. 9, pp. 759–767.

    Article  CAS  Google Scholar 

  24. Volodkin, D.V., Petrov, A.I., Prevot, M., and Sukhorukov, G.B., Langmuir, 2004, vol. 20, pp. 3398–3406.

    Article  CAS  Google Scholar 

  25. Gao, C., Leporatti, S., Moya, S., Donath, E., and Mohwald, H., Langmuir, 2001, vol. 17, pp. 3491–3495.

    Article  CAS  Google Scholar 

  26. Koolman, J. and Rohm, K.-H., Naglyadnaya biokhimiya (Taschenatlas Der Biochemie), Moscow: Mir, 1998.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Stavropol State University, ul. Pushkina 1, Stavropol, 355009, Russia

    T. N. Borodina & S. M. Kunizhev

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

    L. D. Rumsh & E. A. Markvicheva

  3. Queen Mary University of London, London, UK

    G. B. Sukhorukov

  4. Federal State Unitary Enterprise “State Scientific Centre ‘NIOPIK’”, Moscow, Russia

    G. N. Vorozhtsov & B. M. Feldman

Authors
  1. T. N. Borodina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. D. Rumsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. M. Kunizhev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. B. Sukhorukov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. N. Vorozhtsov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. M. Feldman
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. A. Markvicheva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. N. Borodina.

Additional information

Original Russian Text © T.N. Borodina, L.D. Rumsh, S.M. Kunizhev, G.B. Sukhorukov, G.N. Vorozhtsov, B.M. Feldman, E.A. Markvicheva, 2008, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Borodina, T.N., Rumsh, L.D., Kunizhev, S.M. et al. Polyelectrolyte microcapsules as the systems for delivery of biologically active substances. Biochem. Moscow Suppl. Ser. B 2, 88–93 (2008). https://doi.org/10.1007/s11828-008-1010-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11828-008-1010-3

Key words

Search

Navigation