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Identification of human liver cytochromes P450 by using MALDI-TOF mass spectrometry

  • Proteomics
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Abstract

Proteomic approaches have been used for detection and identification of cytochromes P450 forms from highly purified membrane preparations of human liver. These included the protein separation by 2D-and/or 1D-electrophoresis and molecular scanning of a SDS-PAGE gel fragment in a range 45–66 kDa (this area corresponds molecular weights of cytochromes P450). The analysis of protein content was statistically evaluated by means of an original 1D-ZOOMER software package which allowed to carry out the processing of mass spectra mixture instead of individual mass spectra used by standard techniques. In the range 45–66 kDa we identified 13 microsomal membrane proteins including such cytochrome P450 forms as CYPs 1A2, 1B1, 2A6, 2E1, 2C8, 2C9, 2C10, 2D6, 3A4, 4A11, 4F2. Study of enzymatic activities of human liver microsomal cytochrome P450 isoforms CYP 1A, 2B, 3A, and 2E revealed the decrease in the rates of O-dealkylation and N-demethylation catalyzed by CYP 450 1A1/1A2 and 3A4 under pathological conditions, whereas 7-benzyloxyresorufin-O-debenzylase activity (which characterizes the total activity of CYP 2B and CYP 2C), the activities of CYP 2E1 (methanol oxidation), 7-pentoxyresorufin-O-dealkylation (CYP 2B), 7-ethoxy-and 7-methoxycoumarin-O-dealkylases (CYP 2B1) remained basically unchanged.

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References

  1. Lewis, D.F.V., Guide to Cytochrome P450. Structure and Function, London and New York: Taylor and Francis, 2001.

    Book  Google Scholar 

  2. Archakov, A.I. and Bachmanova, G.I., Cytochrome P450 and Active Oxygen, London: Taylor and Francis, 1990.

    Google Scholar 

  3. Rozman, D. and Waterman, M.R., Drug Metab. Dispos., 1998, vol. 26, pp. 1199–1201.

    CAS  Google Scholar 

  4. Whitoclock, J.P.J. and Denison, M.S., in Cytochrome P450: Structure, Mechanism, Biochemistry, Ortiz de Montellano, P.R., Ed., New York: Plenum Press, 1995, p. 391.

  5. Patterson, L.H. and Murray, G.J., Curs: Pharm. Des., 2002, vol. 8, pp. 1335–1347.

    CAS  Google Scholar 

  6. McFadyen, M.C., Melvin, W.T., and Murray, G.I., Mol. Cancer Ther, 2004, vol. 3, pp. 363–371.

    CAS  Google Scholar 

  7. Maurice, M., Emiliani, S., Dalet-Beluche, I., Derancourt, J., and Lange, R., Eur J. Biochem., 1991, vol. 200, pp. 511–517.

    Article  CAS  Google Scholar 

  8. Burke, M.D. and Mayer, R.T., Chem.-Biol. Interact., 1983, vol. 45, pp. 243–258.

    Article  CAS  Google Scholar 

  9. Hover, C.G. and Kulkarni, A.P., Chem. Biol. Interact., 2000, vol. 124, pp. 191–203.

    Article  CAS  Google Scholar 

  10. Wasinger, V.C., Cordwell, S.J., Cerpa-Poljak, A., Yan, J.X., Gooley, A.A., Wilkins, M.R., Duncan, M.W., Harris, R., Williams, K.L., and Humphery-Smith, I., Electrophoresis, 1995, vol. 16, pp. 1090–1094.

    Article  CAS  Google Scholar 

  11. Humphery-Smith, I., Cordwell, S.J., and Blackstock, WP., Electrophoresis, 1997, vol. 18, pp. 1217–1242.

    Article  CAS  Google Scholar 

  12. Gerber, S.A., Rush, J., Stemman, O., Kirschner, M.W., and Gygi, S.P., Proc. Natl. Acad. Sci. USA, 2003, vol. 1000, pp. 6940–6945.

    Article  Google Scholar 

  13. Gygi, S.P., Rist, B., Gerber, S.A., Turecek, F., Gelb, M.H., and Aebersold, R., Nat. Biotechnol., 1999, vol. 17, pp. 994–999.

    Article  CAS  Google Scholar 

  14. Vlasuk, G.P. and Walz, F.G.J., Arch. Biochem. Biophys., 1982, vol. 214, pp. 248–259.

    Article  CAS  Google Scholar 

  15. Archakov, A.I., Bachmanova, G.I., Blinder, L.V., and Kanaeva, I.P., Biokhimiya, 1977, vol. 42, pp. 100–112.

    CAS  Google Scholar 

  16. Omura, T. and Sato, R., J. Biol. Chem., 1964, vol. 239, pp. 2379–2385.

    CAS  Google Scholar 

  17. Pearce, R.E., McIntyre, C.J., Madan, A., Sanzgiri, U., Draper, A.J., Bullock, PL., Cook, D.C., Burton, A., Latham, J., Nevins, C., and Parkinson, A., Arch. Biochem. Biophys., 1996, vol. 331, pp. 145–169.

    Article  CAS  Google Scholar 

  18. French, J.S. and Coon, M.G., Arch. Biochem. Biophys., 1979, vol. 195, pp. 565–577.

    Article  CAS  Google Scholar 

  19. Lowry, O.H., Rosebrough, N.I., Farr, A.L., and Randall, R.I., J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    CAS  Google Scholar 

  20. Yin, H., Tran, P., Grenberg, G.E., and Fischer, V., Drug Metabolism Disposition, 2001, vol. 29, pp. 185–193.

    CAS  Google Scholar 

  21. Govorun, V.M., Moshkovsky, S.A., Tikhonova, O.V., Goufman, E.I., Serebryakova, M.V., Momynaliev, K.T., Lokhov, P.G., Khryaspova, E.V., Kudryavtseva, L.V., Smirnova, O.V., Toropygine, I.Yu., Maksimov, B.I., and Archakov, A.I., Biochemistry (Moscow), 2003, vol. 68, pp. 52–60.

    Article  Google Scholar 

  22. Neuhoff, V., Arold, N., Taube, D., and Ehrhardt, W., Electrophoresis, 1988, vol. 9, pp. 255–262.

    Article  CAS  Google Scholar 

  23. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M., Anal. Chem., 1996, vol. 68, pp. 850–858.

    Article  CAS  Google Scholar 

  24. Perkins, D.N., Pappin, D.D.J., Creasy, D.M., and Cottrell, J.M., Electrophoresis, 1999, vol. 20, pp. 3551–3567.

    Article  CAS  Google Scholar 

  25. Kanaeva, I.P., Petushkova, N.A., Lokhov, P.G., Zgoda, VG., Karuzina, I.I., Lisitsa, A.V., and Archakov, A.I., Biomed. Khim., 2004, vol. 50, pp. 367–375.

    CAS  Google Scholar 

  26. Binz, PA., Muller, M., and Hoogland, C., Curs: Opin. Biotechnol., 2004, vol. 15, pp. 17–23.

    CAS  Google Scholar 

  27. Parent, R. and Beretta, L., J. Hepatology, 2005, vol. 43, pp. 177–183.

    Article  CAS  Google Scholar 

  28. Santoni, V., Molloy, M., and Rabilloud, T., Electrophoresis, 2000, vol. 21, pp. 1054–1070.

    Article  CAS  Google Scholar 

  29. Anzenbacher, P. and Anzenbacherova, E., Cellular Mollecular Life Sciences, 2001, vol. 58, pp. 737–747.

    Article  CAS  Google Scholar 

  30. Graham, D.R.M., Elliott, S.T., and Van Eyk, J.E., J. Physiol., 2005, vol. 563, pp. 1–9.

    Article  CAS  Google Scholar 

  31. Fridman, E. and Pichersky, E., Curr Opin. Plant Biol., 2005, vol. 8, pp. 242–248.

    Article  CAS  Google Scholar 

  32. Lafaye, A., Junot, C., Pereira, Y., Lagniel, G., Tabet, J.-C., Ezan, E., and Labarre, J., J. Biol. Chem., 2005, vol. 280, pp. 24723–24730.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Orekchovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul. 10, Moscow, 119121, Russia

    N. A. Petushkova, A. V. Lisitsa, I. I. Karuzina, V. G. Zgoda, N. F. Samenkova, I. P. Nikitin, T. A. Sakharova, A. T. Kopylov & A. I. Archakov

  2. National Research Center of Surgery, Russian Academy of Medical Sciences, Abrikosovskii per. 2, Moscow, 119992, Russia

    G. F. Sheremetyeva

Authors
  1. N. A. Petushkova
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  2. A. V. Lisitsa
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  3. I. I. Karuzina
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  4. V. G. Zgoda
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  5. G. F. Sheremetyeva
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  6. N. F. Samenkova
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  7. I. P. Nikitin
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  8. T. A. Sakharova
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  9. A. T. Kopylov
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  10. A. I. Archakov
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Correspondence to N. A. Petushkova.

Additional information

Original Russian Text © N.A. Petushkova, A.V. Lisitsa, I.I. Karuzina, V.G. Zgoda, G.F. Sheremetyeva, N.F. Samenkova, I.P. Nikitin, T.A. Sakharova, A.T. Kopylov, A.I. Archakov, 2008, published in Biomeditsinskaya Khimiya.

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Petushkova, N.A., Lisitsa, A.V., Karuzina, I.I. et al. Identification of human liver cytochromes P450 by using MALDI-TOF mass spectrometry. Biochem. Moscow Suppl. Ser. B 2, 47–54 (2008). https://doi.org/10.1007/s11828-008-1004-1

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  • DOI: https://doi.org/10.1007/s11828-008-1004-1

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