Abstract
β-Conglycinin (7S) and glycinin (11S) are the two major storage proteins in soybean seeds. Storage proteins occupy a large portion of soybean seeds, which is the main obstacle to accumulating additional foreign proteins. Thus, we produced 7S-edited soybean plants using CRISPR/Cas9 via Agrobacterium-mediated transformation. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis confirmed that the α′-subunit (76 kDa) of β-conglycinin protein disappeared from the 7S-edited T2 line (#19–8). The removal of the band was caused by the early termination of the CG-1 gene by insertion/deletion (in/del) mutations with an efficiency of 78.5% generated by genome editing. Soybeans with low-storage proteins could be used for improved foreign protein production in seed.
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Acknowledgements
This research was supported by MEDI PROZEN Co., Ltd, the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (RS-2023-00239683), Biomaterials Specialized Graduate Program through the Korea Environmental Industry & Technology Institute (KEITI) funded by the Ministry of Environment (MOE), the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1A6A1A03047729), and the New Breeding Technologies Development Program of the Rural Development Administration (RS-2022-RD009977), Republic of Korea.
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Ha, D.H., Kim, H.J. Mutation of storage protein gene using CRISPR/Cas9 removed α′-subunit of β-conglycinin in soybean seeds. Plant Biotechnol Rep 17, 939–945 (2023). https://doi.org/10.1007/s11816-023-00880-3
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DOI: https://doi.org/10.1007/s11816-023-00880-3