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Effectiveness of sequence-related amplified polymorphism (SRAP) markers to assess the geographical origin and genetic diversity of collected lentil genotypes

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Abstract

Lentil is an important annual food legume crop, nitrogen fixer and provides a substantial amount of protein, carbohydrate, minerals, and vitamin content. The use of molecular markers to assess genetic diversity is crucial for crop improvement, efficient management, and conservation of plant genetic resources. The current study aimed to determine the genetic diversity among lentil genotypes using sequence-related amplified polymorphism (SRAP) markers. Therefore, we evaluated a collection of 36 lentil genotypes, including 20 from Yemen, Saudi Arabia (7), Egypt (4), and Bangladesh (3), and (2) genotypes from the International Center for Research in Dry Area (ICARDA) using 21 SRAP primer combinations. The amplified fragments showed a high level of useful polymorphic amplified fragments (775 out of 782) indicating a higher degree of variation. The polymorphic information content (PIC) ranged from 0.31 to 0.39 with an average of 0.33 for each primer. The UPGMA trees, based on Jaccard similarity index matrices, separated the genotypes into four main clusters according to their geographical origin. The population structure supported the major groups and attested to their great degree of differentiation. The highest lentil population was found at K = 3, K = 5, and K = 7 levels, showing purity and admixture ancestry among the lentil population. This study highlighted the wide genetic diversity among the studied lentil genotypes and demonstrated the effectiveness of the SRAP technique in determining the genetic variability of lentil. Furthermore, it could be used to establish the genetic peculiarity of ecotypes when applying for the obtainment of origin and agro-morphological characteristics.

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Acknowledgements

The authors thank the Deanship of Scientific Research, College of Food and Agricultural Sciences, King Saud University, Saudi Arabia for support and cooperation during the completion of the present research.

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Authors and Affiliations

  1. Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

    Nabil A. Mohammed, Muhammad Afzal, Sulieman A. Al-Faifi, Muhammad A. Khan, Yahya A. Refay, Bazel H. AL-Samin, Salem S. Alghamdi & Abdullah Ibrahim

  2. Department of Agricultural, Faculty of Agriculture and Veterinary, Medicine, Thamar University, Dhamar, Yemen

    Nabil A. Mohammed

  3. Agricultural Research & Extension Authority (AREA), 87148, Dhamar, Yemen

    Bazel H. AL-Samin

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  1. Nabil A. Mohammed
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  2. Muhammad Afzal
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  3. Sulieman A. Al-Faifi
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  4. Muhammad A. Khan
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  7. Salem S. Alghamdi
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Correspondence to Muhammad Afzal.

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Mohammed, N.A., Afzal, M., Al-Faifi, S.A. et al. Effectiveness of sequence-related amplified polymorphism (SRAP) markers to assess the geographical origin and genetic diversity of collected lentil genotypes. Plant Biotechnol Rep 17, 519–530 (2023). https://doi.org/10.1007/s11816-023-00842-9

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