Abstract
Coriander, Coriandrum sativum L., is an important medicinal plant belonging to the Apiaceae family, which is grown in many parts of Turkey. In this study, seven Turkish coriander varieties (Antakya, Arslan, Erbaa, Gurbuz, Kerkuk, Kudret-K, and Pelmus) were examined for phylogenetic relations using two ITS region (ITS4–ITS5) sequence information and their secondary structure analysis. The length of the nrDNA ITS sequences obtained in this study varied between 677 and 692 nucleotides for seven samples. The G + C content of the nrDNA ITS region ranged between 47.86 (ITS4) and 48.67% (ITS5). Secondary structural analysis revealed that variation between varieties is more unique. The AMOVA study confirmed the effectiveness of configuration analysis as variants accounted for the majority of the variance. In addition, variation within cultivars was significant (97%). This shows that a significant part of the variance detected in this study occurred among genotypes. Data analysis, including Shannon information index and scaled diversity overlap graph annotation, also showed that the highest level of heterozygosity occurred in the Arslan, Erbaa and Kudret-K genotypes, with cultivars showing more pronounced differences genotypically rather than geographically, consistent with the expected heterozygosity (80%). By verification of the PCoA analysis, the genotypes of Gurbuz and Arslan are very different from those of other groups. This supports the results of the cluster analysis, which showed that the Ankara-origin Gurbuz variety had a very different genotypic difference. Our analysis recommends the use of ITS4 sequence and secondary structure data at the intraspecific levels of coriander taxonomical classification. For the first time, a study has used both sequence information and secondary structural information to describe coriander at the varietal level of taxonomic classification.
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Acknowledgements
The authors extend their appreciation for providing necessary research facilities and support for the completion of this work to Van Yuzuncu Yil University. We would like to express our gratitude to Prof. Dr. Abdullah Yesilova, the University of Van Yuzuncu Yil, and Associate Prof. Gazel Ser for their assistance with statistical data analysis.
Funding
Support for this work was supplied by Van Yuzuncu Yil University, Scientific Research Projects Coordination Unit (project code FBA-2019-8004) and Basic Science Research Program supported this research through the National Research Foundation of Korea (NRF), funded by the Ministery of Education (2019R1A6A1A11052070).
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MAF and MDK carried out the molecular genetic analysis and preparation of the DNA samples, respectively. MAF conceptualized the experiment, curated the data and registered the sequences in the databank. MAF carried out the data analysis and wrote the first draft of the manuscript. MY and YSC reviewed and contribute to the rewriting and edited the manuscript.
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Furan, M.A., Yıldız, M., Karatas, M.D. et al. Phylogenetic inference and secondary structure predictions of Turkish genotypes of Coriandrum sativum (L.) based on ITS4 and ITS5 nrDNA sequences. Plant Biotechnol Rep 16, 709–720 (2022). https://doi.org/10.1007/s11816-022-00802-9
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DOI: https://doi.org/10.1007/s11816-022-00802-9