Abstract
Common buckwheat is widely cultivated worldwide. Due to its bioactive compounds, there are vigorous studies on protein and metabolism. However, there are not many studies using genetic approaches. Especially researches on genetic diversity in common buckwheat germplasm are rare, although it is crucial for breeding better cultivars. Thus, the current study examined the genetic diversity of the randomly selected common buckwheat germplasm found in the Republic of Korea. The results show that there are not many genetic variances among those wild common buckwheat germplasm that were used in the current study. Although it needs to test more number of germplasm, it seems that more diversity is required to be able to breed cultivars for the future.
References
Aufhammer W (2000) Pseudogetreidearten-buchweizen, reismelde und amarant: herkunft, nutzung und anbau; 167 tabellen. Ulmer, Germany
Earl DA, VonHoldt BM (2012) Structure harvester: a website and program for visualizing structure output and implementing the evanno method. Conserv Genet Resour 4:359–361. https://doi.org/10.1007/s12686-011-9548-7
Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES, Mitchell SE (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6:e19379. https://doi.org/10.1371/journal.pone.0019379
Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
Jhingan JL (1992) A novel technology for DNA isolation. Methods Mol Cell Biol 3:15–22
Johns MA, Skroch PW, Nienhuis J, Hinrichsen P, Bascur G, Muñoz-Schick C (1997) Gene pool classification of common bean landraces from chile based on RAPD and morphological data. Crop Sci 37:605–613. https://doi.org/10.2135/cropsci1997.0011183X003700020049x
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547. https://doi.org/10.1093/molbev/msy096
Lee TH, Guo H, Wang X, Kim C, Paterson AH (2014) SNPhylo: a pipeline to construct a phylogenetic tree from huge SNP data. BMC Genom 15:1–6. https://doi.org/10.1186/1471-2164-15-162
McKenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, Daly M (2010) The genome analysis toolkit: a mapreduce framework for analyzing next-generation DNA sequencing data. Genome Res 20:1297–1303. https://doi.org/10.1101/gr.107524.110
Melo AT, Bartaula R, Hale I (2016) GBS-SNP-CROP: a reference-optional pipeline for SNP discovery and plant germplasm characterization using variable length, paired-end genotyping-by-sequencing data. BMC Bioinform 17:1–15. https://doi.org/10.1186/s12859-016-0879-y
Pirzadah TB, Rehman RU (2021) Buckwheat: forgotten crop for the future: issues and challenges. CRC Press, USA
Robinson DA, Thomas JC, Hanage WP (2011) 3–Population Structure of Pathogenic Bacteria. Genetics and Evolution of Infectious Disease, 2nd edn. Elsevier, Netherlands, pp 43–57
Suzuki T, Noda T, Morishita T, Ishiguro K, Otsuka S, Brunori A (2020) Present status and future perspectives of breeding for buckwheat quality. Breed Sci 70:48–66. https://doi.org/10.1270/jsbbs.19018
Yasui Y, Hirakawa H, Ueno M, Matsui K, Katsube-Tanaka T, Yang SJ, Aii J, Sato S, Mori M (2016) Assembly of the draft genome of buckwheat and its applications in identifying agronomically useful genes. DNA Res 23:215–224. https://doi.org/10.1093/dnares/dsw012
Zhou M, Tang Y, Deng X, Ruan C, Kreft I, Tang Y, Wu Y (2018) Overview of buckwheat resources in the world. Elsevier, Netherlands
Acknowledgements
This research was supported by a grant from the Standardization and integration of resources information for seed-cluster in Hub-Spoke material bank program (Project No. PJ01587004), Rural Development Administration, Republic Korea. We also thank Jeju Buckwheat Farmers Association Corporation, Jeju, Republic of Korea.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Park, J.E., Kang, Y., Han, G.D. et al. Diversity study of common buckwheat germplasm in the Republic of Korea using GBS. Plant Biotechnol Rep 16, 799–803 (2022). https://doi.org/10.1007/s11816-022-00801-w
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11816-022-00801-w