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In planta production and characterization of full-length human adipose triglyceride lipase

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Abstract

Adipose triglyceride lipase (ATGL) is an enzyme that plays a critical role in the mobilization of stored triacylglycerol. Despite its importance in lipid metabolism, in-depth biochemical characterization of ATGL has been limited because the expression and purification of this enzyme are not feasible. In this study, we attempted to generate the full-length human ATGL in Nicotiana benthamiana. The ATGL expressed in N. benthamiana was highly soluble and stable. The purified ATGL exhibited lipase activity in vitro. In conclusion, the full-length human ATGL could be reliably produced using a plant-based expression system, thereby enabling detailed biochemical characterization of this enzyme.

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References

  • Burnett MJB, Burnett AC (2020) Therapeutic recombinant protein production in plants: challenges and opportunities. Plants People Planet 2:121–132

    Article  Google Scholar 

  • Buyel JF (2018) Plant molecular farming—integration and exploitation of side streams to achieve sustainable biomanufacturing. Front Plant Sci 9:1893

    Article  Google Scholar 

  • Cerk IK, Wechselberger L, Oberer M (2018) Adipose triglyceride lipase regulation: an overview. Curr Protein Pept Sci 19:221–233

    CAS  Google Scholar 

  • Chandra P, Enespa, Singh R, Arora PK (2020) Microbial lipases and their industrial applications: a comprehensive review. Microb Cell Fact 19:1–42

    Article  Google Scholar 

  • Geem KR, Song Y, Hwang I, Bae HJ, Lee DW (2021) Production of gloeophyllum trabeum endoglucanase Cel12A in Nicotiana benthamiana for cellulose degradation. Front Plant Sci 12:696199

    Article  Google Scholar 

  • Islam MR, Kwak JW, Lee JS, Hong SW, Khan MRI, Lee Y, Lee Y, Lee SW, Hwang I (2019a) Cost-effective production of tag-less recombinant protein in Nicotiana benthamiana. Plant Biotechnol J 17:1094–1105

    Article  CAS  Google Scholar 

  • Islam MR, Son N, Lee J, Lee DW, Sohn EJ, Hwang I (2019b) Production of bacteriophage-encoded endolysin, LysP11, in Nicotiana benthamiana and its activity as a potent antimicrobial agent against Erysipelothrix rhusiopathiae. Plant Cell Rep 38:1485–1499

    Article  CAS  Google Scholar 

  • Islam MR, Choi S, Muthamilselvan T, Shin K, Hwang I (2020) In vivo removal of N-Terminal fusion domains from recombinant target proteins produced in Nicotiana benthamiana. Front Plant Sci 11:440

    Article  Google Scholar 

  • Jenkins CM, Mancuso DJ, Yan W, Sims HF, Gibson B, Gross RW (2004) Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities. J Biol Chem 279:48968–48975

    Article  CAS  Google Scholar 

  • Kang H, Park Y, Lee Y, Yoo YJ, Hwang I (2018) Fusion of a highly N-glycosylated polypeptide increases the expression of ER-localized proteins in plants. Sci Rep 8:4612

    Article  Google Scholar 

  • Kulminskaya N, Radler C, Viertlmayr R, Heier C, Hofer P, Colaco-Gaspar M, Owens RJ, Zimmermann R, Schreiber R, Zechner R, Oberer M (2021) Optimized expression and purification of adipose triglyceride lipase improved hydrolytic and transacylation activities in vitro. J Biol Chem 297:101206

    Article  CAS  Google Scholar 

  • Nagaya S, Kawamura K, Shinmyo A, Kato K (2010) The HSP terminator of Arabidopsis thaliana increases gene expression in plant cells. Plant Cell Physiol 51:328–332

    Article  CAS  Google Scholar 

  • Razzak MA, Lee DW, Lee J, Hwang I (2020) Overexpression and purification of gracilariopsis chorda carbonic anhydrase (GcCAalpha3) in Nicotiana benthamiana, and its immobilization and Use in CO2 hydration reactions. Front Plant Sci 11:563721

    Article  Google Scholar 

  • Ruiz C, Falcocchio S, Xoxi E, Pastor FI, Diaz P, Saso L (2004) Activation and inhibition of Candida rugosa and Bacillus-related lipases by saturated fatty acids, evaluated by a new colorimetric microassay. Biochim Biophys Acta 1672:184–191

    Article  CAS  Google Scholar 

  • Shanmugaraj B, Bulaon CJI, Phoolcharoen W (2020) Plant molecular farming: a viable platform for recombinant biopharmaceutical production. Plants (Basel) 9:842

  • Villena JA, Roy S, Sarkadi-Nagy E, Kim KH, Sul HS (2004) Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids. J Biol Chem 279:47066–47075

    Article  CAS  Google Scholar 

  • Watt MJ, Spriet LL (2010) Triacylglycerol lipases and metabolic control: implications for health and disease. Am J Physiol Endocrinol Metab 299:E162-168

    Article  CAS  Google Scholar 

  • Yang A, Mottillo EP (2020) Adipocyte lipolysis: from molecular mechanisms of regulation to disease and therapeutics. Biochem J 477:985–1008

    Article  CAS  Google Scholar 

  • Zimmermann R, Strauss JG, Haemmerle G, Schoiswohl G, Birner-Gruenberger R, Riederer M, Lass A, Neuberger G, Eisenhaber F, Hermetter A, Zechner R (2004) Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase. Science 306:1383–1386

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by the grant NRF-2020R1A2C4002294 from the National Research Foundation, Ministry of Science, Technology, and Future Planning, Korea, and “Regional Innovation Strategy (RIS)” through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (MOE) (2021RIS-002). We would like to thank Editage (www.editage.co.kr) for English language editing.

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Author notes

  1. Kseniia Zaikova and Jinseung Jeong have contributed equally to this work.

Authors and Affiliations

  1. Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, South Korea

    Kseniia Zaikova, Jinseung Jeong & Dong Wook Lee

  2. Department of Bioenergy Science and Technology, Chonnam National University, Gwangju, 61186, South Korea

    Kyoung Rok Geem & Dong Wook Lee

  3. Department of Horticulture, Chonnam National University, Gwangju, 61186, South Korea

    Kang-Mo Ku

  4. Department of Chemistry, Chonnam National University, Gwangju, 61186, Korea

    Jeong-Sun Kim

  5. Bio-Energy Research Center, Chonnam National University, Gwangju, 61186, Korea

    Dong Wook Lee

Authors
  1. Kseniia Zaikova
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  2. Jinseung Jeong
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  3. Kyoung Rok Geem
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  4. Kang-Mo Ku
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Correspondence to Dong Wook Lee.

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Zaikova, K., Jeong, J., Geem, K.R. et al. In planta production and characterization of full-length human adipose triglyceride lipase. Plant Biotechnol Rep 16, 793–797 (2022). https://doi.org/10.1007/s11816-022-00799-1

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  • DOI: https://doi.org/10.1007/s11816-022-00799-1

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