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Genome editing provides a valuable biological toolkit for soybean improvement

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Abstract

Soybean [Glycine max (L.) Merr.] is a major economic crop and is used as food, animal feed, and biofuel because of its high contents of oil and proteins. Improvements in soybean quality and yield have so far been achieved using traditional and molecular breeding, including hybridization, mutagenesis, and the insertion of transgenes. However, the breeding of new soybean varieties was unexpectedly prolonged by genetic limitations, such as genomic duplication and redundancy, and complicated social issues caused by the insertion of transgenes. The use of genome-editing technologies for the genetic manipulation of soybean has revolutionized the study of genetic variations, as well as soybean improvement, over the past few years. Here, we summarize the applications of genome editing technologies including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein nuclease 9 (Cas9), in soybean. We discuss targeted gene mutagenesis using current genome-editing technologies, with a focus on the implementation and potential availability of new technical developments in soybean. The accurate, user-friendly approach afforded by CRISPR/Cas9 technology will not only facilitate functional studies of soybean genes but also accelerate soybean breeding for future soybean improvement.

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Funding

This work was supported by grants from the New Breeding Technologies Development Program (Project No. PJ01476901 and PJ01653302), Rural Development Administration, Republic of Korea.

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Author notes

  1. Authors Dongwon Baek and Hyun Jin Chun equally contributed to this study.

Authors and Affiliations

  1. Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 52828, Korea

    Dongwon Baek & Min Chul Kim

  2. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Korea

    Hyun Jin Chun & Min Chul Kim

  3. Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, 52828, Korea

    Min Chul Kim

Authors
  1. Dongwon Baek
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  2. Hyun Jin Chun
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  3. Min Chul Kim
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Contributions

D.B. and H.J.C. organized the data and wrote the manuscript. M.C.K. wrote and edited the manuscript.

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Correspondence to Min Chul Kim.

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Baek, D., Chun, H.J. & Kim, M.C. Genome editing provides a valuable biological toolkit for soybean improvement. Plant Biotechnol Rep 16, 357–368 (2022). https://doi.org/10.1007/s11816-022-00778-6

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  • DOI: https://doi.org/10.1007/s11816-022-00778-6

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