Abstract
Small cysteine-rich peptides (CRPs) are important in plant responses to biotic and abiotic stresses. OsDT11, encoding an 88 amino acid CRP-type protein, has been reported to enhance tolerance to drought in rice (Oryza sativa L.) without negatively affecting other agronomic traits. However, the molecular mechanisms of OsDT11-mediated drought tolerance are still unclear. Here, we performed RNA-Seq analysis to compare the transcriptome profiles between wild-type (WT) and OsDT11-overexpressing (OE) rice lines under drought stress or under control (non-drought) conditions. A total of 1570 and 1421 differentially expressed genes were identified in the OE lines and the WT under drought treatment, respectively, compared to non-drought conditions. Gene ontology analysis indicated that the 430 up-regulated genes in common to both OE and the WT lines were induced for functions related to responses to water deprivation and to abscisic acid (ABA). More than half of these genes had higher expression in the OE than in the WT under drought stress. In the OE, but not in the WT, 294 genes were specifically up-regulated under drought stress and were functionally enriched in starch and sucrose biosynthetic processes and in response to stress. This implies that OsDT11 not only triggers strongly response to drought stress, but also alters several metabolic processes to enhance drought tolerance. Gene expression profiling suggests that OsDT11 confers drought tolerance by mediating an enhanced response to drought stress in an ABA-dependent signaling pathways.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (31700219, 31771748, 31872925), the Natural Science Fund for Outstanding Young Scholars of Shandong Province (JQ201807), the Shandong Modern Agricultural Technology and Industry system (SDAIT-17-06), and Shandong provincial key research and development plan (2019GNC106152).
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11816_2020_637_MOESM1_ESM.tif
Fig. S1 Venn diagrams of differentially expressed genes (DEGs) in OE and WT under normal and drought stress. DEGs were identified with adjusted p-value <0.01. Venn diagrams representing distribution of up-regulated and down-regulated genes in OE or WT under normal (referred to as OE and WT, respectively) and drought stress (referred to as OED and WTD, respectively) (TIF 99 kb)
11816_2020_637_MOESM2_ESM.tif
Fig. S2. The correlation coefficient between RNA-seq and qRT-PCR data. The fold changes in gene expression by qRT-PCR were transformed to log2 scale. The correlation of the qRT-PCR log2 values (x-axis) with RNA-seq data log2-values (y-axis) of (A) 12 common changed genes and (B) 10 specially changed genes in OE. (TIF 65 kb)
11816_2020_637_MOESM3_ESM.tif
Fig. S3. Negative control that is not regulated by OsDT11. GFP fluorescence assay of expanded ayammetrical NB leaves infiltrated with OsDT11 and the constucts(p4, pLOC_Os04g37950 :: GFP) at 48 hpi. Bars = 200 μm. (TIF 113 kb)
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Zhao, M., Ju, Y., Zhao, B. et al. Transcriptional profiling analysis of OsDT11-mediated ABA-dependent signal pathway for drought tolerance in rice. Plant Biotechnol Rep 14, 613–626 (2020). https://doi.org/10.1007/s11816-020-00637-2
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DOI: https://doi.org/10.1007/s11816-020-00637-2