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Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus

Plant Biotechnology Reports volume 12, pages 127–137 (2018)Cite this article

Abstract

To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well.

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Acknowledgements

We are grateful to Dr. G. Lomonossoff (John Innes Centre, UK) for providing the pEAQ-HT plasmid containing the modified non-translated regions from CPMV. We also thank Dr. D. Becker (University of Hamburg, Germany) for the pGmhsp-GUS construct carrying the HSP17.5-E promoter.

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Authors and Affiliations

  1. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants (JKI), Institute for Biosafety in Plant Biotechnology, Erwin-Baur-Str 27, 06484, Quedlinburg, Germany

    L. Kopertekh & E. Krebs

  2. Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino 60 cuadras Km 5½, 5020ICA, Córdoba, Argentina

    F. Guzmann

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  1. L. Kopertekh
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  2. E. Krebs
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  3. F. Guzmann
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Correspondence to L. Kopertekh.

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Kopertekh, L., Krebs, E. & Guzmann, F. Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus. Plant Biotechnol Rep 12, 127–137 (2018). https://doi.org/10.1007/s11816-018-0477-8

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  • DOI: https://doi.org/10.1007/s11816-018-0477-8

Keywords

  • CPMV non-translated regions
  • Potato
  • Site-specific recombination
  • Vegetative progeny