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Investigation of karyotypic composition and evolution in Lilium species belonging to the section martagon

Plant Biotechnology Reports volume 11, pages 407–416 (2017)Cite this article

Abstract

Analyzing chromosomal traits is one of the pragmatic ways to establish evolutionary and genetic database of plants that has complicated phylogenetic system. There are some conflicts on the exact phylogeny and evolutionary pathway of Lilium, and section martagon is the most complicated part among them. In this study, chromosomal traits of martagon lily species are described. All martagon lilies were analyzed with FISH (Fluorescence in situ hybridization) technique, followed by detailed karyotyping. Each species showed 2n = 2x = 24 of chromosome complement. Size of chromosomes ranged from 451.04 to 680.06 µm. 5S and 45S ribosomal DNA, general molecular markers in modern evolutionary research were used as probe in this study. Variation in rDNA loci and chromosome translocation were observed in Lilium hansonii; the highest number of 45S rDNA loci was detected in Lilium hansonii, followed by other martagon lilies, in similar locations but with differences, and chromosome translocation was observed from one individual of Lilium hansonii. Additionally, Lilium tsingtauense from Jeju-do Island, Korea was detected with two extra chromosomes. These kind of genetic variations through karyotyping indicate ongoing genetic variations in martagon lilies. In this study, precise analysis of chromosome traits in Lilium species belonging to section martagonperformed to contribute to better comprehension of the evolutionary pathway and establishment of cytogenetic database for further plant breeding research.

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Acknowledgements

This research was supported by Golden Seed Project (Center for Horticultural Seed Development, no. 213003-04-4-WTM11), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and Korea Forest Service (KFS). And this research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Project no. NRF-2016R1D1A1B04932913).

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Authors and Affiliations

  1. Department of Horticulture, Kyungpook National University, Daegu, 41566, Korea

    Yun-Jae Ahn, Fahad Ramzan, Min-Ji Kwon, Chang-Kil Kim & Ki-Byung Lim

  2. Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, Korea

    Ki-Byung Lim

  3. Department of Life Science, Sahmyook University, Seoul, 01795, Korea

    Yoon-Jung Hwang

  4. Institute of Horticultural Sciences, University of Agriculture, Faisalabad, 38040, Pakistan

    Adnan Younis

  5. Jeju Special Self-Governing Provinces Agricultural Research and Extension Services, Seogwipo, 63556, Korea

    Moon-Seok Sung

  6. Floriculture Research Division, National Institute of Horticultural and Herbal Science, Wanju-gun, 55465, Korea

    Yun-Im Kang

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  1. Yun-Jae Ahn
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  2. Yoon-Jung Hwang
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  3. Adnan Younis
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  8. Chang-Kil Kim
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  9. Ki-Byung Lim
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Contributions

First author and all the co-authors made contributions to in this manuscript. Conception or design of the work: Y-JA, Y-JH, AY, M-SS, K-BL. Data collection: Y-JA. Cultivation of samples: Y-JA, M-SS, FR, M-JK. Data analysis and interpretation: Y-JA, Y-JH, K-BL. Drafting the article: Y-JA, Y-IK, C-KK, K-BL. Acquisition of funding: Y-IK, C-KK, K-BL. Critical revision of the article: Y-JA, Y-JH, AY, K-BL. Final approval of the version to be published: Y-JA, C-KK, K-BL.

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Correspondence to Ki-Byung Lim.

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Ahn, YJ., Hwang, YJ., Younis, A. et al. Investigation of karyotypic composition and evolution in Lilium species belonging to the section martagon. Plant Biotechnol Rep 11, 407–416 (2017). https://doi.org/10.1007/s11816-017-0462-7

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  • DOI: https://doi.org/10.1007/s11816-017-0462-7

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