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Simultaneous estimation of saponin contents from soybean seed using Fourier transform infrared spectroscopy and high-performance liquid chromatography analysis

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Abstract

The aim of this study is to determine the feasibility of Fourier transform infrared (FT-IR) spectroscopy for simultaneous determination of saponin contents in different soybean cultivars. In cross validation between predicted content of saponin by PLS regression modeling from FT-IR spectra and measured content by HPLC, total saponin contents were predicted with good accuracy (R 2 ≥ 0.71). In external validation, saponin group Ab (R 2 = 0.88), saponin DDMP-group βg (R 2 = 0.85), total saponin group B (R 2 = 0.88), and total saponin content (R 2 = 0.87) were predicted with good accuracy, while prediction for saponin group Aa (R 2 = 0.58), saponin group Bb′ (R 2 = 0.58), and total saponin group A (R 2 = 0.25) had relatively lower accuracy. Considering these results, we suggest that the PLS prediction system for saponin contents using FT-IR analysis could be applied as a novel screening tool for high yielding lines in soybean breeding.

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Acknowledgements

This work was supported by a Grant (PJ008329 to S.W.K.) from the Next-Generation BioGreen 21 Program of the Rural Development Administration of Korea. In addition, this work was supported by a Grant from the KRIBB Research Initiative Program, a Grant (111161-5) provided to SWK from the Korean Ministry for Agriculture, Food and Rural Affairs and a Grant (K14418) to SWK from the KIOM.

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Authors and Affiliations

  1. Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181, Ipsin-gil, Jeongeup, Jeollabuk-Do, 56212, Korea

    Myung Suk Ahn, Eun Yee Jie & Suk Weon Kim

  2. Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA), 281 Ayeon-ro, Jeju, 63240, Korea

    Seung Yeob Song

  3. College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju, Chungbuk, 28644, Korea

    Hong Sig Kim

  4. Faculty of Biotechnology, Jeju National University, Jeju, 63243, Korea

    In-Jung Kim

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  1. Myung Suk Ahn
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  2. Eun Yee Jie
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Correspondence to Suk Weon Kim.

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Ahn, M.S., Jie, E.Y., Song, S.Y. et al. Simultaneous estimation of saponin contents from soybean seed using Fourier transform infrared spectroscopy and high-performance liquid chromatography analysis. Plant Biotechnol Rep 10, 403–414 (2016). https://doi.org/10.1007/s11816-016-0411-x

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