Skip to main content

Advertisement

SpringerLink
Log in

Regulatory non-coding RNAs in plants: potential gene resources for the improvement of agricultural traits

  • Review Article
  • Published:
Plant Biotechnology Reports Aims and scope Submit manuscript

Abstract

Plant regulatory non-coding RNAs (ncRNAs) are known as important “top tier” regulators in gene regulatory networks. They modulate various biological processes and responses to surrounding environments by directly regulating their target genes which are involved in growth and product yield of crop plants from transcription to translation steps. Due to their agricultural importance of regulatory ncRNAs in plants, they are now spotlighted as potential targets for molecular breeding of agricultural trait-improved crop plants. In this review, we will discuss the agricultural importance of these plant regulatory ncRNAs, and review recent progresses in understanding their functions and molecular mechanisms in regulating their target genes. In addition, we will discuss the application of next generation sequencing (NGS) methodologies on regulatory ncRNA researches in plants, and candidate selection strategy using those NGS-derived dataset and pre-existing genetic materials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Aung K, Lin SI, Wu CC, Huang YT, Su CL, Chiou TJ (2006) pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene. Plant Physiol 141:1000–1011

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Aung B, Gruber MY, Amyot L, Omari K, Bertrand A, Hannoufa A (2015) Ectopic expression of LjmiR156 delays flowering, enhances shoot branching, and improves forage quality in alfalfa. Plant Biotechnol Rep 9:379–393

    Article  Google Scholar 

  • Bari R, Datt Pant B, Stitt M, Scheible WR (2006) PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants. Plant Physiol 141:988–999

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bi YM, Kant S, Clarke J, Gidda S, Ming F, Xu J, Rochon A, Shelp BJ, Hao L, Zhao R, Mullen RT, Zhu T, Rothstein SJ (2009) Increased nitrogen-use efficiency in transgenic rice plants over-expressing a nitrogen-responsive early nodulin gene identified from rice expression profiling. Plant, Cell Environ 32:1749–1760

    Article  CAS  Google Scholar 

  • Bologna NG, Voinnet O (2014) The diversity, biogenesis, and activities of endogenous silencing small RNAs in Arabidopsis. Annu Rev Plant Biol 65:473–503

    Article  CAS  PubMed  Google Scholar 

  • Brodersen P, Sakvarelidze-Achard L, Bruun-Rasmussen M, Dunoyer P, Yamamoto YY, Sieburth L, Voinnet O (2008) Widespread translational inhibition by plant miRNAs and siRNAs. Science 320:1185–1190

    Article  CAS  PubMed  Google Scholar 

  • Campalans A, Kondorosi A, Crespi M (2004) enod40, a short open reading frame-containing mRNA, induces cytoplasmic localization of a nuclear RNA binding protein in Medicago truncatula. Plant Cell 16:1047–1059

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Charon C, Johansson C, Kondorosi E, Kondorosi A, Crespi M (1997) enod40 induces dedifferentiation and division of root cortical cells in legumes. Proc Natl Acad Sci USA 94:8901–8906

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Crespi MD, Jurkevitch E, Poiret M, d’Aubenton-Carafa Y, Petrovics G, Kondorosi E, Kondorosi A (1994) enod40, a gene expressed during nodule organogenesis, codes for a non-translatable RNA involved in plant growth. The EMBO journal 13:5099–5112

    CAS  PubMed  PubMed Central  Google Scholar 

  • Di C, Yuan J, Wu Y, Li J, Lin H, Hu L, Zhang T, Qi Y, Gerstein MB, Guo Y, Lu ZJ (2014) Characterization of stress-responsive lncRNAs in Arabidopsis thaliana by integrating expression, epigenetic and structural features. Plant J Cell Mol Biol 80:848–861

    Article  CAS  Google Scholar 

  • Ding J, Lu Q, Ouyang Y, Mao H, Zhang P, Yao J, Xu C, Li X, Xiao J, Zhang Q (2012) A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice. Proc Natl Acad Sci USA 109:2654–2659

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fan D, Liu T, Li C, Jiao B, Li S, Hou Y, Luo K (2015) Efficient CRISPR/Cas9-mediated targeted mutagenesis in Populus in the first generation. Sci Rep 5:12217

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Franco-Zorrilla JM, Valli A, Todesco M, Mateos I, Puga MI, Rubio-Somoza I, Leyva A, Weigel D, Garcia JA, Paz-Ares J (2007) Target mimicry provides a new mechanism for regulation of microRNA activity. Nat Genet 39:1033–1037

    Article  CAS  PubMed  Google Scholar 

  • Fuentes SI, Allen DJ, Ortiz-Lopez A, Hernandez G (2001) Over-expression of cytosolic glutamine synthetase increases photosynthesis and growth at low nitrogen concentrations. J Exp Bot 52:1071–1081

    Article  CAS  PubMed  Google Scholar 

  • Gu W, Lee HC, Chaves D, Youngman EM, Pazour GJ, Conte D Jr, Mello CC (2012) CapSeq and CIP-TAP identify Pol II start sites and reveal capped small RNAs as C. elegans piRNA precursors. Cell 151:1488–1500

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gupta PK (2015) MicroRNAs and target mimics for crop improvement. Curr Sci India 108:1624–1633

    Google Scholar 

  • Haag JR, Pikaard CS (2011) Multisubunit RNA polymerases IV and V: purveyors of non-coding RNA for plant gene silencing. Nat Rev Mol Cell Biol 12:483–492

    Article  CAS  PubMed  Google Scholar 

  • He XF, Fang YY, Feng L, Guo HS (2008) Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR-NBS-LRR class R gene-derived novel miRNA in Brassica. FEBS Lett 582:2445–2452

    Article  CAS  PubMed  Google Scholar 

  • Heo JB, Sung S (2011) Vernalization-mediated epigenetic silencing by a long intronic noncoding RNA. Science 331:76–79

    Article  CAS  PubMed  Google Scholar 

  • Huang X, Qian Q, Liu Z, Sun H, He S, Luo D, Xia G, Chu C, Li J, Fu X (2009) Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet 41:494–497

    Article  CAS  PubMed  Google Scholar 

  • Hunt AG (2011) RNA regulatory elements and polyadenylation in plants. Frontiers Plant Sci 2:109

    CAS  Google Scholar 

  • Jabnoune M, Secco D, Lecampion C, Robaglia C, Shu Q, Poirier Y (2013) A rice cis-natural antisense RNA acts as a translational enhancer for its cognate mRNA and contributes to phosphate homeostasis and plant fitness. Plant Cell 25:4166–4182

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jeong DH, Green PJ (2012) Methods for validation of miRNA sequence variants and the cleavage of their targets. Methods 58:135–143

    Article  CAS  PubMed  Google Scholar 

  • Jeong DH, Park S, Zhai J, Gurazada SG, De Paoli E, Meyers BC, Green PJ (2011) Massive analysis of rice small RNAs: mechanistic implications of regulated microRNAs and variants for differential target RNA cleavage. Plant Cell 23:4185–4207

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiao Y, Wang Y, Xue D, Wang J, Yan M, Liu G, Dong G, Zeng D, Lu Z, Zhu X, Qian Q, Li J (2010) Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet 42:541–544

    Article  CAS  PubMed  Google Scholar 

  • Kim DH, Sung S (2012) Environmentally coordinated epigenetic silencing of FLC by protein and long noncoding RNA components. Curr Opin Plant Biol 15:51–56

    Article  CAS  PubMed  Google Scholar 

  • Lauressergues D, Couzigou JM, Clemente HS, Martinez Y, Dunand C, Becard G, Combier JP (2015) Primary transcripts of microRNAs encode regulatory peptides. Nat 520:90–93

    Article  CAS  Google Scholar 

  • Levin JZ, Yassour M, Adiconis X, Nusbaum C, Thompson DA, Friedman N, Gnirke A, Regev A (2010) Comprehensive comparative analysis of strand-specific RNA sequencing methods. Nat Methods 7:709–715

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li WX, Oono Y, Zhu J, He XJ, Wu JM, Iida K, Lu XY, Cui X, Jin H, Zhu JK (2008) The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance. Plant Cell 20:2238–2251

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li Y, Zhang Q, Zhang J, Wu L, Qi Y, Zhou JM (2010a) Identification of microRNAs involved in pathogen-associated molecular pattern-triggered plant innate immunity. Plant Physiol 152:2222–2231

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li YF, Zheng Y, Addo-Quaye C, Zhang L, Saini A, Jagadeeswaran G, Axtell MJ, Zhang W, Sunkar R (2010b) Transcriptome-wide identification of microRNA targets in rice. Plant J Cell Mol Biol 62:742–759

    Article  CAS  Google Scholar 

  • Li S, Liu L, Zhuang X, Yu Y, Liu X, Cui X, Ji L, Pan Z, Cao X, Mo B, Zhang F, Raikhel N, Jiang L, Chen X (2013) MicroRNAs inhibit the translation of target mRNAs on the endoplasmic reticulum in Arabidopsis. Cell 153:562–574

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li Y, Lu YG, Shi Y, Wu L, Xu YJ, Huang F, Guo XY, Zhang Y, Fan J, Zhao JQ, Zhang HY, Xu PZ, Zhou JM, Wu XJ, Wang PR, Wang WM (2014) Multiple rice microRNAs are involved in immunity against the blast fungus Magnaporthe oryzae. Plant Physiol 164:1077–1092

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liang G, He H, Yu D (2012) Identification of nitrogen starvation-responsive microRNAs in Arabidopsis thaliana. PLoS ONE 7:e48951

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liang G, Ai Q, Yu D (2015) Uncovering miRNAs involved in crosstalk between nutrient deficiencies in Arabidopsis. Sci Rep 5:11813

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu Q, Chen YQ (2010) A new mechanism in plant engineering: the potential roles of microRNAs in molecular breeding for crop improvement. Biotechnol Adv 28:301–307

    Article  CAS  PubMed  Google Scholar 

  • Luan MD, Xu MY, Lu YM, Zhang QX, Zhang L, Zhang CY, Fan YL, Lang ZH, Wang L (2014) Family-wide survey of miR169s and NF-YAs and their expression profiles response to abiotic stress in maize roots. PloS one 9

  • Mao Y, Zhang H, Xu N, Zhang B, Gou F, Zhu JK (2013) Application of the CRISPR-Cas system for efficient genome engineering in plants. Mol Plant 6:2008–2011

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miao J, Guo D, Zhang J, Huang Q, Qin G, Zhang X, Wan J, Gu H, Qu LJ (2013) Targeted mutagenesis in rice using CRISPR-Cas system. Cell Res 23:1233–1236

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miura K, Ikeda M, Matsubara A, Song XJ, Ito M, Asano K, Matsuoka M, Kitano H, Ashikari M (2010) OsSPL14 promotes panicle branching and higher grain productivity in rice. Nat Genet 42:545–549

    Article  CAS  PubMed  Google Scholar 

  • Miyao A, Iwasaki Y, Kitano H, Itoh J, Maekawa M, Murata K, Yatou O, Nagato Y, Hirochika H (2007) A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes. Plant Mol Biol 63:625–635

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Muthusamy M, Uma S, Backiyarani S, Saraswathi MS (2015) Genome-wide screening for novel, drought stress-responsive long non-coding RNAs in drought-stressed leaf transcriptome of drought-tolerant and -susceptible banana (Musa spp) cultivars using Illumina high-throughput sequencing. Plant Biotechnol Rep 9:279–286

    Article  Google Scholar 

  • Navarro L, Dunoyer P, Jay F, Arnold B, Dharmasiri N, Estelle M, Voinnet O, Jones JD (2006) A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science 312:436–439

    Article  CAS  PubMed  Google Scholar 

  • Ni Z, Hu Z, Jiang Q, Zhang H (2013) GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress. Plant Mol Biol 82:113–129

    Article  CAS  PubMed  Google Scholar 

  • Park JH, Shin C (2015) The role of plant small RNAs in NB-LRR regulation. Briefings in functional genomics 14:268–274

    Article  PubMed  Google Scholar 

  • Quan M, Chen J, Zhang D (2015) Exploring the secrets of long noncoding RNAs. Int J Mol Sci 16:5467–5496

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rinn JL, Chang HY (2012) Genome Regulation by Long Noncoding RNAs. Annu Rev Biochem 81:145–166

    Article  CAS  PubMed  Google Scholar 

  • Ruiz-Ferrer V, Voinnet O (2009) Roles of plant small RNAs in biotic stress responses. Annu Rev Plant Biol 60:485–510

    Article  CAS  PubMed  Google Scholar 

  • Schmidhuber J, Tubiello FN (2007) Global food security under climate change. Proc Natl Acad Sci USA 104:19703–19708

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Secco D, Baumann A, Poirier Y (2010) Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons. Plant Physiol 152:1693–1704

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Secco D, Jabnoune M, Walker H, Shou H, Wu P, Poirier Y, Whelan J (2013) Spatio-temporal transcript profiling of rice roots and shoots in response to phosphate starvation and recovery. Plant Cell 25:4285–4304

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shen YJ, Venu RC, Nobuta K, Wu XH, Notibala V, Demirci C, Meyers BC, Wang GL, Ji GL, Li QSQ (2011) Transcriptome dynamics through alternative polyadenylation in developmental and environmental responses in plants revealed by deep sequencing. Gen Res 21:1478–1486

    Article  CAS  Google Scholar 

  • Shivaprasad PV, Chen HM, Patel K, Bond DM, Santos BA, Baulcombe DC (2012) A microRNA superfamily regulates nucleotide binding site-leucine-rich repeats and other mRNAs. Plant Cell 24:859–874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Silva GFFE, Silva EM, Azevedo MD, Guivin MAC, Ramiro DA, Figueiredo CR, Carrer H, Peres LEP, Nogueira FTS (2014) microRNA156-targeted SPL/SBP box transcription factors regulate tomato ovary and fruit development. Plant Journal 78:604–618

    Article  PubMed  Google Scholar 

  • Sima XJ, Jiang B, Fang J, He YQ, Fang ZX, Kumar KMS, Ren W, Qiu LL, Chen XM, Zheng BS (2015) Identification by deep sequencing and profiling of conserved and novel hickory microRNAs involved in the graft process. Plant Biotechnol Rep 9:115–124

    Article  Google Scholar 

  • Singh D, Jha B (2014) The isolation and identification of salt-responsive novel microRNAs from Salicornia brachiata, an extreme halophyte. Plant Biotechnol Rep 8:325–336

    Article  Google Scholar 

  • Sousa C, Johansson C, Charon C, Manyani H, Sautter C, Kondorosi A, Crespi M (2001) Translational and structural requirements of the early nodulin gene enod40, a short-open reading frame-containing RNA, for elicitation of a cell-specific growth response in the alfalfa root cortex. Mol Cell Biol 21:354–366

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sun Z, He Y, Li J, Wang X, Chen J (2015) Genome-wide characterization of rice black streaked dwarf virus-responsive microRNAs in rice leaves and roots by small RNA and degradome sequencing. Plant Cell Physiol 56:688–699

    Article  PubMed  Google Scholar 

  • Sunkar R, Li YF, Jagadeeswaran G (2012) Functions of microRNAs in plant stress responses. Trends Plant Sci 17:196–203

    Article  CAS  PubMed  Google Scholar 

  • Takeda T, Suwa Y, Suzuki M, Kitano H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M, Ueguchi C (2003) The OsTB1 gene negatively regulates lateral branching in rice. Plant J Cell Mol Biol 33:513–520

    Article  CAS  Google Scholar 

  • Tang GL, Tang XQ (2013) Short tandem target mimic: a long journey to the engineered molecular landmine for selective destruction/blockage of MicroRNAs in plants and animals. J Genet Genomics 40:291–296

    Article  CAS  PubMed  Google Scholar 

  • Valiollahi E, Farsi M, Kakhki AM (2014) Sly-miR166 and Sly-miR319 are components of the cold stress response in Solanum lycopersicum. Plant Biotechnol Rep 8:349–356

    Article  Google Scholar 

  • Wheeler T, von Braun J (2013) Climate change impacts on global food security. Science 341:508–513

    Article  CAS  PubMed  Google Scholar 

  • Wu L, Zhang Q, Zhou H, Ni F, Wu X, Qi Y (2009) Rice MicroRNA effector complexes and targets. Plant Cell 21:3421–3435

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xie K, Yang Y (2013) RNA-guided genome editing in plants using a CRISPR-Cas system. Mol Plant 6:1975–1983

    Article  CAS  PubMed  Google Scholar 

  • Yan F, Guo W, Wu G, Lu Y, Peng J, Zheng H, Lin L, Chen J (2014) A virus-based miRNA suppression (VbMS) system for miRNA loss-of-function analysis in plants. Biotechnol J 9:702–708

    Article  CAS  PubMed  Google Scholar 

  • Yanagisawa S, Akiyama A, Kisaka H, Uchimiya H, Miwa T (2004) Metabolic engineering with Dof1 transcription factor in plants: improved nitrogen assimilation and growth under low-nitrogen conditions. Proc Natl Acad Sci USA 101:7833–7838

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yang W-C, Katinakis P, Hendriks P, Smolders A, Vries F, Spee J, Kammen A, Bisseling T, Franssen H (1993) Characterization of GmENOD40, a gene showing novel patterns of cell-specific expression during soybean nodule development. Plant J 3:573–585

    Article  CAS  PubMed  Google Scholar 

  • Yi H, Richards EJ (2007) A cluster of disease resistance genes in Arabidopsis is coordinately regulated by transcriptional activation and RNA silencing. Plant Cell 19:2929–2939

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhai J, Jeong DH, De Paoli E, Park S, Rosen BD, Li Y, Gonzalez AJ, Yan Z, Kitto SL, Grusak MA, Jackson SA, Stacey G, Cook DR, Green PJ, Sherrier DJ, Meyers BC (2011) MicroRNAs as master regulators of the plant NB-LRR defense gene family via the production of phased, trans-acting siRNAs. Genes Dev 25:2540–2553

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang W, Gao S, Zhou X, Chellappan P, Chen Z, Zhou X, Zhang X, Fromuth N, Coutino G, Coffey M, Jin H (2011a) Bacteria-responsive microRNAs regulate plant innate immunity by modulating plant hormone networks. Plant Mol Biol 75:93–105

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang X, Zou Z, Gong P, Zhang J, Ziaf K, Li H, Xiao F, Ye Z (2011b) Over-expression of microRNA169 confers enhanced drought tolerance to tomato. Biotechnol Lett 33:403–409

    Article  CAS  PubMed  Google Scholar 

  • Zhang XH, Zou Z, Zhang JH, Zhang YY, Han QQ, Hu TX, Xu XG, Liu H, Li HX, Ye ZB (2011c) Over-expression of sly-miR156a in tomato results in multiple vegetative and reproductive trait alterations and partial phenocopy of the sft mutant. FEBS Lett 585:435–439

    Article  CAS  PubMed  Google Scholar 

  • Zhang YC, Yu Y, Wang CY, Li ZY, Liu Q, Xu J, Liao JY, Wang XJ, Qu LH, Chen F, Xin P, Yan C, Chu J, Li HQ, Chen YQ (2013) Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching. Nat Biotechnol 31:848–852

    Article  CAS  PubMed  Google Scholar 

  • Zhang YC, Liao JY, Li ZY, Yu Y, Zhang JP, Li QF, Qu LH, Shu WS, Chen YQ (2014) Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice. Genome Biol 15:512

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhao B, Liang R, Ge L, Li W, Xiao H, Lin H, Ruan K, Jin Y (2007) Identification of drought-induced microRNAs in rice. Biochem Biophys Res Commun 354:585–590

    Article  CAS  PubMed  Google Scholar 

  • Zhao B, Ge L, Liang R, Li W, Ruan K, Lin H, Jin Y (2009) Members of miR-169 family are induced by high salinity and transiently inhibit the NF-YA transcription factor. BMC Mol Biol 10:29

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhao M, Ding H, Zhu JK, Zhang F, Li WX (2011) Involvement of miR169 in the nitrogen-starvation responses in Arabidopsis. New Phytol 190:906–915

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao JP, Jiang XL, Zhang BY, Su XH (2012a) Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa. PLoS ONE 7:e44968

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao M, Tai H, Sun S, Zhang F, Xu Y, Li WX (2012b) Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency. PLoS ONE 7:e29669

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zheng LL, Qu LH (2015) Application of microRNA gene resources in the improvement of agronomic traits in rice. Plant Biotechnol J 13:329–336

    Article  CAS  PubMed  Google Scholar 

  • Zhou M, Luo H (2013) MicroRNA-mediated gene regulation: potential applications for plant genetic engineering. Plant Mol Biol 83:59–75

    Article  CAS  PubMed  Google Scholar 

  • Zhou L, Liu Y, Liu Z, Kong D, Duan M, Luo L (2010) Genome-wide identification and analysis of drought-responsive microRNAs in Oryza sativa. J Exp Bot 61:4157–4168

    Article  CAS  PubMed  Google Scholar 

  • Zhu QH, Wang MB (2012) Molecular functions of long non-coding RNAs in plants. Genes 3:176–190

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

This work was supported by the Next-Generation BioGreen 21 Program (No. PJ01115601), Rural Development Administration, Republic of Korea.

Author information

Authors and Affiliations

  1. Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Republic of Korea

    Sang-Yoon Shin & Chanseok Shin

  2. Interdisciplinary Program in Agricultural Genomics, Seoul National University, Seoul, 151-921, Republic of Korea

    Sang-Yoon Shin & Chanseok Shin

Authors
  1. Sang-Yoon Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chanseok Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chanseok Shin.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shin, SY., Shin, C. Regulatory non-coding RNAs in plants: potential gene resources for the improvement of agricultural traits. Plant Biotechnol Rep 10, 35–47 (2016). https://doi.org/10.1007/s11816-016-0389-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11816-016-0389-4

Keywords

Search

Navigation