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Positive effects of cold pretreatment, iron source, and silver nitrate on anther culture of strawberry (Fragaria × ananassa Duch.)

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Abstract

Androgenesis by anther culture or isolated microspore culture is the most efficient method for haploid production. In this study, the effects of cold pretreatment (4 °C), silver nitrate, and iron source in the medium were investigated on anther culture of five strawberry cultivars (Camarosa, Selva, Pajaro, Paros, and Gaviota) in three independent experiments, and three traits including percentage of androgenic anthers, embryogenesis, and callogenesis were determined. In the first experiment, cold pretreatment of 4 °C for 2 and 3 days produced the highest percentage of androgenic anthers in three cultivars (Camarosa, Selva, and Pajaro) and cold pretreatment of 4 °C for 2 days produced the highest percentage of androgenic anthers in cultivar Paros. In the second experiment, the effect of Fe-EDDHA was more effective than Fe-EDTA, and increased the percentage of androgenic anthers and embryogenesis. The use of 57.5 mg l−1 Fe-EDDHA produced the highest percentage of androgenic anthers in cultivar Camarosa and the best embryogenesis in cultivars Camarosa and Gaviota. In the third experiment, the use of 15 mg l−1 silver nitrate in medium significantly increased the percentage of androgenic anthers and embryogenesis in cultivar Camarosa.

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Abbreviations

IAA:

Indole-3-acetic acid

BAP:

6-Benzylaminopurine

EDTA:

Ethylenediaminetetraacetic acid

EDDHA:

Ethylenediamine di-o-hydroxyphenylacetic acid

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Authors and Affiliations

  1. Department of Plant Breeding and Agricultural Biotechnology, Faculty of Agriculture, Tarbiat Modares University, P.O. Box 14115-336, Tehran, Iran

    Roohollah Shahvali-Kohshour & Ahmad Moieni

  2. Department of Biotechnology, International Center for Science, High Technology and Environmental Science, Kerman, Iran

    Amin Baghizadeh

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  1. Roohollah Shahvali-Kohshour
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  2. Ahmad Moieni
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  3. Amin Baghizadeh
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Correspondence to Ahmad Moieni.

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Shahvali-Kohshour, R., Moieni, A. & Baghizadeh, A. Positive effects of cold pretreatment, iron source, and silver nitrate on anther culture of strawberry (Fragaria × ananassa Duch.). Plant Biotechnol Rep 7, 481–488 (2013). https://doi.org/10.1007/s11816-013-0286-z

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