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Complete sequence and genetic features of the mitochondrial genome of Pyropia tenera (Rhodophyta)

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Abstract

We determined the complete mitochondrial genome (mtDNA, mitogenome) of Pyropia tenera (Bangiales, Rhodophyta). Pyropia tenera mtDNA had a larger size (42,268 bp) than the mtDNA sequences of Porphyra and Pyropia reported previously, and encoded two ribosomal RNA genes [large subunit (rnl), small subunit (rns)], 24 transfer RNAs, four ribosomal proteins, and 17 genes involved in electron transport and oxidative phosphorylation. Moreover, four conserved open reading frames (ORFs) and six intronic ORFs (three in rnl and three in the cox1 gene) were also identified. In comparison with other Porphyra and Pyropia species, Py. tenera had four major structural changes in two gene loss/rearrangement regions [tRNA-Gln(uug)–tRNA-SeC(uca) and tRNA-Ala(ugc)–tRNA-Arg(ucu)] and two different patterns of exon, intron, and intronic ORFs (rnl and cox1). The unique features of Py. tenera mtDNA include the complete sequence of red algal mtDNA for investigating mtDNA evolution and developing molecular markers for species identification. In addition, red algal mtDNA can provide useful genetic information as a genetic reservoir for bioengineering.

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Acknowledgments

This study was supported by a grant (RP-2013-BT-031) from the National Fisheries Research and Development Institute, Korea.

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Authors and Affiliations

  1. Aquatic Plant Variety Center, National Fisheries Research and Development Institute, Mokpo, 530-831, Korea

    Mi Sook Hwang, Seung-Oh Kim & Dong-Soo Ha

  2. Marine Research Institute, Pusan National University, Busan, 609-735, Korea

    Jee Eun Lee & Sang-Rae Lee

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  1. Mi Sook Hwang
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  2. Seung-Oh Kim
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  3. Dong-Soo Ha
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  4. Jee Eun Lee
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  5. Sang-Rae Lee
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Correspondence to Sang-Rae Lee.

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Hwang, M.S., Kim, SO., Ha, DS. et al. Complete sequence and genetic features of the mitochondrial genome of Pyropia tenera (Rhodophyta). Plant Biotechnol Rep 7, 435–443 (2013). https://doi.org/10.1007/s11816-013-0281-4

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  • DOI: https://doi.org/10.1007/s11816-013-0281-4

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