Abstract
Gene promoter(s) specialized in root tissues is an important component for crop biotechnology. In our current study, we report results of promoter analysis of the HPX1, a gene expressed predominantly in roots. The HPX1 promoter regions were predicted, linked to the gfp reporter gene, and transformed into rice. Promoter activities were analyzed in various organs and tissues of six independent transgenic HPX1:gfp plants using the fluorescent microscopy and q-RT-PCR methods. GFP fluorescence levels were high in root elongation regions but not in root apex and cap of the HPX1:gfp plants. Very low levels of GFP fluorescence were observed in anthers and leaves. Levels of promoter activities were 16- to 190-fold higher in roots than in leaves of the HPX1:gfp plants. The HPX1 promoter directs high levels of gene expression in root tissues producing GFP levels up to 0.39 % of the total soluble protein. Thus, the HPX1 promoter is predominantly active in the root elongation region during the vegetative stage of growth.
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Acknowledgments
This study was supported by the Rural Development Administration under the Next-Generation BioGreen 21 Program (Project No. PJ007971 to J.-K.K. and PJ009022 to J.S.J), and by the Ministry of Education, Science and Technology under “Mid-career Researcher Program” (Project No. 20100026168 to J.-K.K.).
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S.-H. Park, J. S. Jeong and E. H. Han are contributed equally to this work.
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Park, SH., Jeong, J.S., Han, E.H. et al. Characterization of the root-predominant gene promoter HPX1 in transgenic rice plants. Plant Biotechnol Rep 7, 339–344 (2013). https://doi.org/10.1007/s11816-012-0268-6
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DOI: https://doi.org/10.1007/s11816-012-0268-6