Plant Biotechnology Reports

, Volume 6, Issue 2, pp 97–106 | Cite as

Biotechnological advances in jojoba [Simmondsia chinensis (Link) Schneider]: recent developments and prospects for further research

  • Sunil Kumar
  • Manisha Mangal
  • A. K. Dhawan
  • Narender Singh
Review Article


Jojoba (Simmondsia chinensis), is a medicinal and oil-yielding, multi-purpose species of the family Simmondsiaceae. The most valuable product of jojoba seed is the liquid wax or jojoba oil which is used extensively in the cosmetic and bio-fuel industry. Propagation of jojoba is possible using conventional methods, but it is time consuming and cumbersome owing to long rotation periods, male-biased population, and long flowering and seed set time. The development of an efficient regeneration system is a prerequisite for a number of biotechnological interventions for the improvement of jojoba, such as genetic transformation, production of useful metabolites in vitro, etc. During the past decade, therefore, several attempts have been made for in vitro propagation of jojoba. Organogenesis has been achieved in this species from mature as well as juvenile explants. Present communication reports an overview of the in vitro regeneration of jojoba via organogenesis and somatic embryogenesis. Factors affecting organogenesis as well as production of synthetic seeds using shoot tips and axillary buds have also been discussed; however, efforts need to be made to develop an efficient genetic transformation system in jojoba. The purpose of this review is to focus upon the current information on in vitro propagation and biotechnological advances made in jojoba.


Biochemical changes In vitro propagation Jojoba Organogenesis Somatic embryogenesis Synthetic seeds 


  1. Agarwal M, Shrivastava N, Padh H (2011) Development of sex-linked AFLP markers in Simmondsia chinensis. Plant Breed 130:114–116CrossRefGoogle Scholar
  2. Agrawal V, Prakash S, Izhar S (1999) Differential hormonal requirements for clonal propagation of male and female jojoba plants. In: Altman A, Ziv M (eds) Plant biotechnology and in vitro biology in the 21st century. Proceedings of the 9th international congress of the international association of plant tissue culture biotechnology, Jerusalem, Israel, 14–19 June 1998, pp 25–28Google Scholar
  3. Agrawal V, Prakash S, Gupta SC (2002) Effective protocol for in vitro shoot production through nodal explants of Simmondsia chinensis. Biol Plant 45:449–453CrossRefGoogle Scholar
  4. Agrawal V, Sharma K, Sarika G, Kumar R, Prasad M (2007) ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis). Plant Biotech Rep 2:239–243Google Scholar
  5. Amarger V, Mercier L (1995) Molecular analysis of RAPD DNA based markers: their potential use for the detection of genetic variability in jojoba (Simmondsia chinensis L Schneider). Biochimie 77:931–936PubMedCrossRefGoogle Scholar
  6. Amin MN, Jaiswal VS (1984) In vitro propagation of guava (Psidium guajava L.): effects of sucrose, agar and pH on growth and proliferation of shoots. Bangladesh J Bot 18:1–8Google Scholar
  7. Amin MN, Jaiswal VS (1987) Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tissue Organ Cult 9:235–243CrossRefGoogle Scholar
  8. Anonymous (1975) Under-exploited tropical plants with promising economic value. National Academy of Sciences. Washington, DCGoogle Scholar
  9. Apostolo NM, Llorente B (2000) Anatomy of normal and hyperhydric leaves and shoots of in vitro grown Simmondsia chinesis (Link) Schn. In Vitro Cell Dev Biol Plant 36:414–418Google Scholar
  10. Apostolo NM, Brutti C, Ferrarotti SA, Llorente BE, Krymkiewicz NL (2001) Stimulation of root development with cyclodextrins on jojoba shoots in vitro. In Vitro Cell Dev Biol Plant 37:414–418Google Scholar
  11. Bajaj YPS (1986) Biotechnology of tree improvement for rapid propagation and biomass energy production. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 1. Springer, Berlin, pp 1–23Google Scholar
  12. Bashir MA, Rashid H, Anjum MA (2007a) In vitro shoot multiplication of six promising strains of jojoba (Simmondsia chinensis). Biotechnology 6:309–315CrossRefGoogle Scholar
  13. Bashir MA, Anjum MA, Rashid H (2007b) In vitro root formation in micropropagated shoots of jojoba (Simmondsia chinensis). Biotechnology 6:465–472CrossRefGoogle Scholar
  14. Bashir MA, Muhammad AA, Hamid R (2008) In vitro propagation of some promising genotypes of jojoba (Simmondsia chinensis). Afr J Biotech 7:3878–3886Google Scholar
  15. Benzioni A (1995) Jojoba domestication and commercialization in Israel. Hortic Rev 17:234–266Google Scholar
  16. Benzioni A, Mills D, Wenkart S, Zhou Y (2003) Effects of ventilation on the performance of jojoba (Simmondsia chinensis) clones: multiplication stage. Acta Hortic 616:135–138Google Scholar
  17. Bhardwaj M, Uppal S, Jain S, Kharb P, Dhillon R, Jain RK (2010) Comparative assessment of ISSR and RAPD marker assays for genetic diversity analysis in jojoba [Simmondsia chinensis (Link) Schneider]. J Plant Biochem Biotechnol 19:255–258Google Scholar
  18. Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice. Elsevier, AmsterdamGoogle Scholar
  19. Birnbaum E, Matias S, Wenkart S (1984) Vegatative propagation of jojoba by tissue culture. In: Wisniak J, Zabicky J (eds) Proceedings of the sixth international conference on jojoba and its uses. Ben Gurion University, Beer-Sheva, pp 233–241Google Scholar
  20. Chaturvedi HC, Sharma M (1989) In vitro production of cloned plants of jojoba (Simmondsia chinensis (Link) Schneider) through shoot proliferation in long term culture. Plant Sci 63:199–207CrossRefGoogle Scholar
  21. Christianson ML (1987) Causal events in morphogenesis. In: Green CE, Somers DA, Hackett WP, Biesboer DD (eds) Plant tissue and cell culture. Liss, New York, pp 44–55Google Scholar
  22. Dewan A, Nanda K, Gupta SC (1992) In vitro propagation of Acacia nilotica subsp Indian breman via cotyledonary nodes. Plant Cell Rep 12:18–21CrossRefGoogle Scholar
  23. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  24. Driver JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. Hortic Sci 19:507–509Google Scholar
  25. Elhag H, El-Olemy MM, Mossa JS, Tag-El-Din SS, Al-Zoghet MF, Al-Alsheikh AMA (1998) In vitro propagation of jojoba. In: Program abstract of the annual conference on new crops and new uses: biodiversity and sustainability, Phoenix, AZGoogle Scholar
  26. Engleman F, Engles J, Dullo E (2003) The development of complementary strategies for the conservation of plant genetic resources using in vitro and cryopreservation methods. In: Chaudhury R, Pandey R, Malik SK, Bhag Mal (eds) In vitro conservation and cryopreservation of tropical fruit species. IPGRI, NBPGR, New Delhi, pp 37–48Google Scholar
  27. Gaber A, El-Maraghy HM, Aly MA, Rashed NA, El-Din AY (2007) Induction of somatic embryogenesis and DNA fingerprinting of jojoba. Arab J Biotechnol 10:341–354Google Scholar
  28. Gao HD, Cao B (2001) Study on technology of tissue culture of Simmondsia chinensis (Link) Schneider (in Chinese). J Jiangsu For Sci Technol 28:12–14Google Scholar
  29. Gautheret RJ (1955) Nutrition of plant cultures. Annu Rev Plant Physiol 6:435–484CrossRefGoogle Scholar
  30. Giri C, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees Struct Funct 18:115–135CrossRefGoogle Scholar
  31. Gulati A, Jaiwal PK (1996) Micropropagation of Dalbergia sissoo from nodal explant of mature trees. Biol Plant 38:169–175CrossRefGoogle Scholar
  32. Gupta AK (2001) Jojoba seed and oil from Indian locations for quality. In: Proceeding of national seminar on production, marketing and processing of jojoba (Simmondsia chinensis), Jaipur, India, (abstr.), pp 40–41Google Scholar
  33. Haberlandt G (1902) Culturversuche mit isolierten. Pflanzenzellen Sitzungsber Akad Wiss Wien Math Nat Cl III Abt, vol 1, pp 69–91Google Scholar
  34. Hamama L, Baaziz M, Letouze R (2001) Somatic embryogenesis and plant regeneration from leaf tissue of jojoba. Plant Cell Tissue Organ Cult 65:109–113CrossRefGoogle Scholar
  35. Harris PJC, Moore THM, Puddephat IJ (1989) Micropropagation of Sesbania sesban. Nitrogen Fixing Tree Res Rep 7:123–125Google Scholar
  36. Harsh LN, Tiwari JC, Bohra MD, Tripathi D (2001) Standardization of agronomic practices of jojoba cultivation in arid regions. In: Proceeding of national seminar on production, marketing and processing of jojoba (Simmondsia chinensis), Jaipur, India, (abstr.), pp 31–32Google Scholar
  37. Hassan NS (2003) In vitro propagation of jojoba (Simmondsia chinensis L) through alginate-encapsulated shoot apical and axillary buds. Int J Agric Biol 5:513–516Google Scholar
  38. Hosseini FS, Hassani HS, Arvin MJ, Baghizadeh A, Nejad GM (2011) Sex determination of jojoba (Simmondsia chinensis cv. Arizona) by random amplified polymorphic DNA (RAPD) molecular markers. Afr J Biotechnol 10:470–474Google Scholar
  39. Ince AG, Karaca MA, Onus AN (2010) A reliable gender diagnostic PCR assay for jojoba (Simmondsia chinensis (Link) Schneider). Genet Resour Crop Evol 57:773–779CrossRefGoogle Scholar
  40. Jacoboni A, Standardi A (1987) Tissue culture of jojoba (Simmondsia chinensis Link). Acta Hortic 212:557–560Google Scholar
  41. John A, Murray BW (1981) Micropropagation of sitka spruce (Picea sitchensis). In: Colloque international surla culture in vitro des essences, Fountainebleau, AFOCEL, pp 65–70Google Scholar
  42. Kacker NL, Joshi SP, Singh M, Solanki KR (1993) In vitro regeneration of female plants of Simmondsia chinensis (Link) Schneider (Jojoba) using coppice shoots. Ann Arid Zone 32:175–177Google Scholar
  43. Karp A (1994) Origin, causes and uses of variation in plant tissue cultures. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Kluwer, Dordrecht, pp 139–150Google Scholar
  44. Keathley DE (1984) Micropropagation of mature spruce. In: Karnosky H, Keathly DE (eds) International symposium on recent advances in forest biotechnology. Traverse City, Michigan, pp 58–63Google Scholar
  45. Kim SW, Liu JR (1999) Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower. Plant Cell Tissue Organ Cult 58:227–230CrossRefGoogle Scholar
  46. Komamine A, Murata N, Nomura K (2005) Mechanisms of somatic embryogenesis in carrot suspension cultures-morphology, physiology, biochemistry, and molecular biology. In Vitro Cell Dev Biol Plant 41:6–10CrossRefGoogle Scholar
  47. Krishna H, Singh SK (2007) Biotechnological advances in mango (Mangifera indica L.) and their future implication in crop improvement—a review. Biotechnol Adv 25:223–243PubMedCrossRefGoogle Scholar
  48. Kumar S, Singh N, Mangal M (2009a) Biochemical changes during shoot differentiation in callus culture of jojoba (Simmondsia chinensis). J Plant Biol 36:11–16Google Scholar
  49. Kumar S, Singh N, Mangal M (2009b) Micropropagation of Simmondsia chinensis (Link) Schneider through enhanced axillary branching from nodal segments. J Plant Biol 36:75–81Google Scholar
  50. Kumar N, Modi AR, Singh AS, Gajera BB, Patel AR, Patel MP, Subhash N (2010a) Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD & ISSR markers assay. Physiol Mol Biol Plants 16:207–213CrossRefGoogle Scholar
  51. Kumar S, Rai MK, Singh N, Mangal M (2010b) Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution. Physiol Mol Biol Plants 16:379–382CrossRefGoogle Scholar
  52. Kumar S, Mangal M, Dhawan AK, Singh N (2011) Assessment of genetic fidelity of micropropagated plants of Simmondsia chinensis (Link) Schneider using RAPD and ISSR markers. Acta Physiol Plant. doi:10.1007/s11738-011-0767-z
  53. Lee CW (1988) Application of plant biotechnology for clonal propagation and yield enhancement in jojoba. In: Baldwin AR (ed) Proceeding of the seventh international conference on jojoba and its uses. Illinois, USA, pp 102–111Google Scholar
  54. Lee CW, Palzkill DA (1984) Propagation of jojoba by single node cutting. Hortic Sci 19:841–842Google Scholar
  55. Lee CW, Thomas JC (1985) Jojoba embryo culture and oil production. Hortic Sci 20:762–764Google Scholar
  56. Litz RE, Gray DJ (1992) Organogenesis and somatic embryogenesis. In: Hammerschlag FA, Litz RE (eds) Biotechnology of perennial fruit crops. CAB International, Wallingford, pp 3–34Google Scholar
  57. Llorente B, Apostolo NM (1998) Effect of different growth regulators and genotype on in vitro propagation of jojoba. N Z J Crop Hortic Sci 26:55–62CrossRefGoogle Scholar
  58. Low CB, Hackett WP (1981) Vegetative propagation of jojoba. Calif Agric 35:12–13Google Scholar
  59. Malik SK, Chaudhury R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Sci Hortic 106:539–553CrossRefGoogle Scholar
  60. Mandani A, Lee CW, Hogan L (1978) In vitro propagation of Simmondsia chinensis via shoot tip culture. Hortic Sci 13:35–37Google Scholar
  61. McCown B, Amos R (1979) Initial trials with commercial micropropagation of birch selections. Proc Int Plant Prop Soc 29:387–393Google Scholar
  62. Meyghani H, Ghazvini RF, Hamidoghli Y (2005) Micropropagation from stem segments of salt tolerant jojoba seedlings. J Korean Soc Hortic Sci 46:83–187Google Scholar
  63. Mills D, Benzioni A (1992) The effect of NaCl salinity on growth and development of jojoba clones: II. Nodal segments grown in vitro. J Plant Physiol 139:737–741CrossRefGoogle Scholar
  64. Mills D, Wenkart S, Benzioni A (1997) Micropropagation of Simmondsia chinensis (Jojoba). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 40. High-tech and micropropagation VI. Springer, Berlin, pp 370–393Google Scholar
  65. Mills D, Yanqing Z, Benzioni A (2004) Improvement of jojoba shoot multiplication in vitro by ventilation. In Vitro Cell Dev Biol Plant 40:396–402CrossRefGoogle Scholar
  66. Mohammed AM, Aly Essam A, Amer Wasef A, Al-Zayadneh AE, Negm E (2008) Growth regulators influence the fatty acid profiles of in vitro induced jojoba somatic embryos. Plant Cell Tissue Organ Cult 93:107–114CrossRefGoogle Scholar
  67. Murashige T (1974) Plant propagation through tissue cultures. Annu Rev Plant Physiol 25:135–166CrossRefGoogle Scholar
  68. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  69. Naik SK, Chand PK (2006) Nutrient-alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci Hortic 108:247–252CrossRefGoogle Scholar
  70. Nandwani D, Ramawat KG (1993) In vitro plantlet formation through juvenile and mature explants in Prosopis cineraria. Indian J Exp Biol 3:156–160Google Scholar
  71. Nehra NS, Kartha KK (1994) Meristem and shoot tip culture: requirement and application. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Kluwer, Dordrecht, pp 37–70Google Scholar
  72. Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose—a review. Biotechnol Adv 24:94–114PubMedCrossRefGoogle Scholar
  73. Phillips RL, Kaeppler SM, Olhoft P (1994) Genetic instability of plant tissue cultures: breakdown of normal controls. Proc Natl Acad Sci USA 91:5222–5226PubMedCrossRefGoogle Scholar
  74. Philomina NS, Rao JVS (1999) Multiple shoots production from seed culture of soap nut (Sapindus mukorossi Gaertn.). Phytomorphology 49:419–423Google Scholar
  75. Prakash S, Agrawal V, Gupta SC (2003) Influence of some adjuvants on in vitro clonal propagation of male and female jojoba plants. In Vitro Cell Dev Biol Plant 39:217–222CrossRefGoogle Scholar
  76. Raghava Swamy BV, Himabindu K, Sita GL (1992) In vitro micropropagation of elite rose wood (Dalbergia latifolia Roxb.). Plant Cell Rep 11:126–131Google Scholar
  77. Rai MK, Asthana P, Singh SK, Jaiswal VS, Jaiswal U (2009) The encapsulation technology in fruit plants—a review. Biotechnol Adv 27:671–679PubMedCrossRefGoogle Scholar
  78. Rai MK, Asthana P, Jaiswal VS, Jaiswal U (2010) Biotechnological advances in guava (Psidium guajava L.): recent development and prospects for future research. Trees 24:1–12CrossRefGoogle Scholar
  79. Rao PS, Suprasanna P, Ganapathi TR (1996) Plant biotechnology and agriculture prospects for improvement and increasing plant productivity. Sci Cult 62:185–191Google Scholar
  80. Reddy MP, Chikara J (2010) Biotechnology advances in jojoba (Simmondsia chinensis). In: Ramawat KG (ed) Desert plants. Springer, Berlin, pp 407–422CrossRefGoogle Scholar
  81. Roja G, Rao PS (1998) Biotechnological investigation in medicinal plants for the production of secondary metabolites. In: Khan I, Khanum A (eds) Role of biotechnology in medicinal and aromatic plants. Ukaaj, Hyderabad, pp 95–125Google Scholar
  82. Rost TL, Hinchee MAW (1980) Preliminary report of the production of callus, organogenesis and regeneration of jojoba (Simmondsia chinensis (Link) Schneider) in tissue culture. J Hortic Sci 55:299–305Google Scholar
  83. Roussos PA, Tolia-Marioli A, Pontikis CA, Kotsias D (1999) Rapid multiplication of jojoba seedling by in vitro culture. Plant Cell Tissue Organ Cult 57:133–137CrossRefGoogle Scholar
  84. Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18:91–120PubMedCrossRefGoogle Scholar
  85. Rumary C, Thorpe TA (1984) Plantlet formation in black and white spruce. Can J Res 14:10–16CrossRefGoogle Scholar
  86. Saiprasad GVS (2001) Artificial seeds and their application. Resonance 39–47Google Scholar
  87. Sardana J, Batra A (1998) In vitro regeneration of jojoba (Simmondsia chinensis): a plant of high potential. Adv Plant Sci 11:143–146Google Scholar
  88. Scaramuzzi F, D’Ambrosio A (1988) Organogenesis and propagation in vitro of Simmondsia chinensis (Link) Schn (jojoba) from vegetative fragments. Acta Hortic 227:411–413Google Scholar
  89. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204CrossRefGoogle Scholar
  90. Shahzad A, Siddiqui SA (2001) Micropropagation of Melia azedarach L. Phytomorphology 51:151–154Google Scholar
  91. Sharma K, Agrawal V, Sarika G, Kumar R, Prasad M (2008) ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis). Plant Biotechnol Rep 2:239–243CrossRefGoogle Scholar
  92. Sharma R, Chowdhury V, Jain S, Jain RK (2009) A comparative study of genetic relationships among and within male and female genotypes of dioecious jojoba (Simmondsia chinensis L. Schneider) using RAPD and ISSR markers. Asian J Hortic 4:184–193Google Scholar
  93. Singh M, Jaiswal U, Jaiswal VS (2004) In vitro regeneration and improvement in tropical fruit trees: an assessment. In: Srivastava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. Anamanya, New Delhi, pp 228–243Google Scholar
  94. Singh A, Reddy MP, Patolia JS (2008) An improved protocol for micropropagation of elite genotype of Simmondsia chinensis (Link) Schneider. Biol Plant 52:538–542CrossRefGoogle Scholar
  95. Singh SR, Singh R, Dhawan AK (2009) Biochemical changes related to shoot differentiation in callus cultures of Tylophora indica wight and arn. J Indian Bot Soc 88:49–53Google Scholar
  96. Smith DL, Krikorian AD (1991) Growth and maintenance of an embryogenic cell culture of daylily (Hemerocallis) on hormone free medium. Ann Bot 67:443–447PubMedGoogle Scholar
  97. Thakur M, Sharma DR, Kanwar K (2001) Mass micropropagation of Alnus nepalensis D. Don. Phytomorphology 51:123–127Google Scholar
  98. Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631PubMedCrossRefGoogle Scholar
  99. Tyagi RK, Prakash S (2004) Genotype and sex specific protocols for in vitro micropropagation and medium term conservation of jojoba. Biol Plant 48:119–123Google Scholar
  100. Undersander DJ, Oelke EA, Kaminski AR, Doll JD, Putnam DH, Combs SM, Hanson CV (1990) Jojoba.
  101. Wang YC, Janick J (1986a) Somatic embryogenesis in jojoba. J Am Soc Hortic Sci 111:281–287Google Scholar
  102. Wang YC, Janick J (1986b) In vitro production of jojoba liquid wax by zygotic and somatic embryos. J Am Soc Hortic Sci 111:798–806Google Scholar
  103. Zimmerman JL (1993) Somatic embryogenesis: a model of early development in higher plants. Plant Cell 5:1411–1423PubMedCrossRefGoogle Scholar

Copyright information

© Korean Society for Plant Biotechnology and Springer 2011

Authors and Affiliations

  • Sunil Kumar
    • 1
    • 2
  • Manisha Mangal
    • 3
  • A. K. Dhawan
    • 4
  • Narender Singh
    • 2
  1. 1.Centre for Plant BiotechnologyCCS Haryana Agricultural University New CampusHisarIndia
  2. 2.Department of BotanyKurukshetra UniversityKurukshetraIndia
  3. 3.Central Institute of Post-Harvesting Engineering and TechnologyLudhianaIndia
  4. 4.Regional Research StationCCS Haryana Agricultural UniversityKarnalIndia

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