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Characterization of phenolic compounds biosynthesized in pink-colored skin of Japanese indigenous Vitis vinifera cv. Koshu grape

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Abstract

Vitis vinifera cv. Koshu is a traditional grape cultivar that has been grown for centuries in Japan. The Koshu grape has pink-colored skin and Koshu wines have slight astringency. We demonstrated for the first time the characterization of hydroxycinnamic acids, flavan-3-ols, and flavonoids in Koshu grape using high-performance liquid chromatography and liquid chromatography–mass spectrometry. The gross weight of phenolic compounds excluding anthocyanins and proanthocyanidins in Koshu grape at harvest was higher than those in Sauvignon Blanc, Chardonnay, and Merlot grapes. In addition, hydroxycinnamic acid and monomeric flavonol contents in Koshu grape were also higher than those in the other grape cultivars. Transcription analysis of cinnamic acid 4-hydroxylase, p-coumarate 3-hydroxylase, caffeate methyltransferase, and flavonol synthase genes indicated high accumulation of hydroxycinnamic acids and flavonols in Koshu grape skin compared with the other cultivars. These findings obtained by chemical and molecular approaches partially explained the phenolic characteristics and the peculiar astringency of Koshu grape.

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Authors and Affiliations

  1. Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, 1-13-1, Kofu, Yamanashi, 400-0005, Japan

    Hironori Kobayashi & Shunji Suzuki

  2. Château Mercian, Mercian Corporation, 1425-1 Shimoiwasaki, Katsunuma, Koshu, Yamanashi, 409-1313, Japan

    Hironori Kobayashi, Kosei Ajimura & Hiroshi Saito

  3. Product Development Research Laboratory, Mercian Corporation, 4-9-1 Johnan, Fujisawa, Kanagawa, 251-0057, Japan

    Yumiko Suzuki & Tomonori Konno

Authors
  1. Hironori Kobayashi
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  2. Yumiko Suzuki
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  3. Kosei Ajimura
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  4. Tomonori Konno
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  5. Shunji Suzuki
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  6. Hiroshi Saito
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Correspondence to Shunji Suzuki.

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Kobayashi, H., Suzuki, Y., Ajimura, K. et al. Characterization of phenolic compounds biosynthesized in pink-colored skin of Japanese indigenous Vitis vinifera cv. Koshu grape. Plant Biotechnol Rep 5, 79–88 (2011). https://doi.org/10.1007/s11816-010-0162-z

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  • DOI: https://doi.org/10.1007/s11816-010-0162-z

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