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High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.)

Abstract

An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g l−1 sucrose, 2.2 g l−1 Gelrite, and 7.7 μM naphthalene acetic acid (NAA) with 2.2 μM thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30–40 shoots per explant) was achieved on MS medium containing 5.5 μM TDZ, 2.2 μM NAA, and 3.3 μM silver nitrate (AgNO3). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 μM gibberellic acid (GA3). The elongated shoots were rooted in MS medium supplemented with 4.0 μM indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days.

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Abbreviations

AgNO3 :

Silver nitrate

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-Benzylaminopurine

FAA:

Formaldehyde acetic acid alcohol

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog medium

NAA:

α-Naphthaleneacetic acid

TDZ:

Thiadiazuron

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Correspondence to Muthu Thiruvengadam.

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Thiruvengadam, M., Rekha, K.T., Yang, CH. et al. High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.). Plant Biotechnol Rep 4, 321–328 (2010). https://doi.org/10.1007/s11816-010-0151-2

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Keywords

  • Adventitious shoots
  • Growth regulators
  • Organogenic callus
  • Hardening