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Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant

Plant Biotechnology Reports volume 4, pages 261–267 (2010)Cite this article

Abstract

We describe culture conditions for a high-efficiency in vitro regeneration system of Papaver nudicaule through somatic embryogenesis and secondary somatic embryogenesis. The embryogenic callus induction rate was highest when petiole explants were cultured on Murashige and Skoog (MS) medium containing 1.0 mg l−1 α-naphthaleneacetic acid (NAA) and 0.1 mg l−1 6-benzyladenine (BA) (36.7%). When transferred to plant growth regulator (PGR)-free medium, 430 somatic embryos formed asynchronously from 90 mg of embryogenic callus in each 100-ml flask. Early-stage somatic embryos were transferred to MS medium containing 1.0 mg l−1 BA and 1.0 mg l−1 NAA to germinate at high frequency (97.6%). One-third-strength MS medium with 1.0% sucrose and 1.0 mg l−1 GA3 had the highest frequency of plantlet conversion from somatic embryos (91.2%). Over 90% of regenerated plantlets were successfully acclimated in the greenhouse. Secondary somatic embryos were frequently induced directly when the excised hypocotyls of the primary somatic embryos were cultured on MS medium without PGRs. Sucrose concentration significantly affected the induction of secondary embryos. The highest induction rate (89.5) and number of secondary somatic embryos per explant (9.3) were obtained by 1% sucrose. Most secondary embryos (87.2–94.3%) developed into the cotyledonary stage on induction medium. All cotyledonary secondary embryos were converted into plantlets both in liquid and on semisolid 1/3-strength MS medium with 1.0% sucrose.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30671701).

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Authors and Affiliations

  1. Key Laboratory of Forest Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China

    Jing Li Yang & Cheng Hao Li

  2. Pharmacy College, Heilongjiang University of Traditional Chinese Medicine, Harbin, 150086, China

    Bo Zhao

  3. Bioherb Research Institute, Kangwon National University, Chuncheon, 200-701, South Korea

    Eun Soo Seong, Myong Jo Kim & Chang Yeon Yu

  4. Department of Horticulture, Kangwon National University, Chunchon, 200-701, South Korea

    Won Hee Kang

  5. Food Service Cuisine, Songho College, Hoingsung, 225-704, South Korea

    Na Young Kim

Authors
  1. Jing Li Yang
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  2. Bo Zhao
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  3. Eun Soo Seong
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  4. Myong Jo Kim
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  5. Won Hee Kang
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  6. Na Young Kim
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  7. Chang Yeon Yu
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  8. Cheng Hao Li
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Corresponding authors

Correspondence to Chang Yeon Yu or Cheng Hao Li.

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J. L. Yang and B. Zhao contributed equally to this work.

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Yang, J.L., Zhao, B., Seong, E.S. et al. Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant. Plant Biotechnol Rep 4, 261–267 (2010). https://doi.org/10.1007/s11816-010-0144-1

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  • DOI: https://doi.org/10.1007/s11816-010-0144-1

Keywords

  • Papaveraceae
  • Germination
  • Embryogenic callus
  • Somatic embryo
  • Secondary somatic embryo