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An efficient in vitro plant regeneration system for the medicinal plant Teucrium stocksianum Boiss.

Abstract

An efficient and rapid plant regeneration system via direct organogenesis was established for Teucrium stocksianum Boiss. (Lamiaceae), an endangered and valuable medicinal plant. Hypocotyl explants excised from seedlings germinated in vitro were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of kinetin and indoleacetic acid (IAA) to induce shoot formation. Differentiation of multiple shoots was initiated within 3 weeks of culture. Optimal regeneration was achieved on medium containing 3 mg/l kinetin and 0.5 mg/l IAA. This particular medium composition significantly improved the production of multiple shoots directly from hypocotyl explants compared to other combinations of plant growth regulators. Root induction was achieved on half-strength MS medium containing indole-3-butyric acid. Rooted plantlets were successfully acclimatized, with a survival rate of 75–80%. The protocol developed in this study could be used for long-term in vitro conservation and mass propagation of this species.

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References

  • Ahmad H, Ahmad A, Jan MM (2002) The medicinal plants of salt range. J Biol Sci 2:175–177

    Article  Google Scholar 

  • Chen CC, Chen SJ, Sagare AP, Tsay HS (2001) Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae)—an important medicinal herb. Bot Bull Acad Sin 42:1–7

    Google Scholar 

  • Debnath M, Malik CP, Bisen PS (2006) Micropropagation: a tool for the production of high quality plant-based medicines. Curr Pharm Biotechnol 7:33–49

    PubMed  Article  CAS  Google Scholar 

  • Fay MF (1992) Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev Biol Plant 28:1–4

    Google Scholar 

  • Huang CL, Hsieh MT, Hsieh WC, Sagare AP, Tsay HS (2000) In vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot tip, leaf- and inflorescence-node explants. In Vitro Cell Dev Biol Plant 36:220–224

    Article  CAS  Google Scholar 

  • Iqbal I, Hamayun M (2004) Studies on the traditional uses of plants of Malam Jabba valley, District Swat, Pakistan. Ethnobotanical Leaflets; International Web Journal, pp 1–30, 12 May 2006. http://www.siu.edu/∼ebl/index2004.htm

  • Islam MW, Zakaria MNM, Radhakrishnan R, Kamil M, Chan KC, Al-Attas A (2002) Effect of Teucrium stocksianum on gastric ulceration and secretion in rats. Pharm Biol 40:216–220

    Google Scholar 

  • Jayaram K, Prasad MNV (2007) Rapid in vitro multiplication of Drosera indica L.: a vulnerable, medicinally important insectivorous plant. Plant Biotechnol Rep 1:79–84

    Article  Google Scholar 

  • Jogbloed MVD (2003) The comprehensive guide to the wild flowers of the United Arab Emirates. Environmental Research for Wildlife Development Agency, United Arab Emirates

    Google Scholar 

  • Joshi M, U Dhar (2003) In vitro propagation of Saussurea obvallata (DC.) Edgew.—an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Rep 21:933–939

    PubMed  Article  CAS  Google Scholar 

  • Liu CZ, Murch SJ, El-Demerdash M, Saxena PK (2003) Regeneration of the Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep 21:525–530

    PubMed  CAS  Google Scholar 

  • Makunga NP, Jager AK, Staden J van (2003) Micropropagation of Thapsia gargania—a medicinal plant. Plant Cell Rep 21:967–973

    PubMed  Article  CAS  Google Scholar 

  • Martin KP (2003) Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep 21:415–420

    PubMed  CAS  Google Scholar 

  • Mojab F, Javidnia K, Yazdani D, Rustaiynan A (2003) Essential oil of the aerial parts of Teucrium stocksianum Boiss subsp. stocksianum (Lamiacea) from Iran. J Med Plants 2:49–53

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nadaf SK, Al-Farsi SM, Al-Hinai SA (2003) Germplasm collection of rangeland forage and medicinal plant species in north Oman. In: Annu Rep ICARDA–APRP. ICARDA–APRP, Dubai, pp 63–64

  • Radhakrishnan R, Zakaria MNM, Islam MW, Kamil M, Ismail A, Chan K, Al-Attas A (2001) Analgesic and anti-inflammatory activities of Teucrium stocksianum. Pharm Biol 39:455–459

    Article  Google Scholar 

  • Rasheed RA, Ali BH, Bachir AK (1995) Effect of Teucrium stocksianum on paracetamol-induced hepatotoxicity in mice. Gen Pharmacol 26:297–301

    PubMed  Article  CAS  Google Scholar 

  • Schmeda-Hirschmann G, Jordan M, Gerth A, Wilken D, Hormazabal E, Tapia AA (2004) Secondary metabolite content in Fabiana imbricata plants and in vitro cultures. Z Naturforsch 59:48–54

    CAS  Google Scholar 

  • Shinwari ZK, Gilani SS (2003) Sustainable harvest of medicinal plants of Astore, Northern Pakistan. J Ethnopharmacol 2:289–298

    Article  Google Scholar 

  • Van Huylenbroeck CJM, Debergh PC (1995) Physiological aspects in acclimatization of micropropagated plants. Plant Tissue Biotechnol 2:136–141

    Google Scholar 

  • Van Huylenbroeck JM, Piqueras A, Debergh PC (1998) Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plants. Plant Sci 137:21–30

    Article  Google Scholar 

  • Wawrosh PR, Kopp MB (2001) Clonal propagation of Lilium nepalense D Don., a threatened medicinal plant of Nepal. Plant Cell Rep 20:285–288

    Article  CAS  Google Scholar 

  • Western AR (1989) The flora of the United Arab Emirates, an introduction. United Arab Emirates University, Al Ain

    Google Scholar 

  • Xu X, Yu R, Zhao Y (2004) Establishment of plantlet regeneration system of medicinal plant Aconitum coreanum. Zhong Yao Cai 27:797–799

    PubMed  Google Scholar 

  • Yamada Y, Shoyama Y, Nishioka I, Kohda H, Namera A, Okamoto T (1991) Clonal micropropagation of Gentiana scabra Bunge var. buergeri Maxim. and examination of the homogeneity concerning the gentiopicroside content. Chem Pharm Bull 39:204–206

    CAS  Google Scholar 

  • You XL, Han JY, Choi YE (2007) Plant regeneration via direct somatic embryogenesis in Panax japonicus. Plant Biotechnol Rep 1:5–9

    Article  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Research Affairs at the UAE University under a contract no. 06-04-2-11/ 05.

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Correspondence to Naima Bouhouche.

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Bouhouche, N., Ksiksi, T. An efficient in vitro plant regeneration system for the medicinal plant Teucrium stocksianum Boiss.. Plant Biotechnol Rep 1, 179–184 (2007). https://doi.org/10.1007/s11816-007-0033-4

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  • DOI: https://doi.org/10.1007/s11816-007-0033-4

Keywords

  • Medicinal plant
  • Micropropagation
  • Organogenesis
  • Teucrium stocksianum
  • Tissue culture