Abstract
Jatropha curcas L. is one potential source of non-edible biofuel-producing energy crop. Its importance also lies in its medicinal properties. The species is primarily propagated through heterozygous seeds, and thus the seed oil content varies from 4 to 40%. Moreover, due to its perennial nature, seed setting requires 2 to 3 years time. The seed viability and rate of germination are low, and quality seed screening is another laborious task; thus, seed propagation alone cannot provide quality planting material for sustainable use. Somatic embryogenesis, a powerful tool of plant biotechnology for faster and quality plant production has been successfully applied to regenerate plants in Jatropha curcas for the first time. Embryogenic calli were obtained from leaf explants on MS basal medium supplemented with only 9.3 μM Kn. Induction of globular somatic embryos from 58% of the cultures was achieved on MS medium with different concentrations of 2.3–4.6 μM Kn and 0.5–4.9 μM IBA; 2.3 μM Kn and 1.0 μM IBA proved to be the most effective combination for somatic embryo induction in Jatropha curcas. Addition of 13.6 μM adenine sulphate stimulated the process of development of somatic embryos. Mature somatic embryos were converted to plantlets on half strength MS basal medium with 90% survival rate in the field condition. The whole process required 12–16 weeks of culture for completion of all steps of plant regeneration. This protocol of somatic embryogenesis in Jatropha curcas may be an ideal system for future transgenic research.
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References
Bhansali R (1990) Somatic embryogenesis and regeneration of plantlets in pomegranate. Ann Bot 66:249–254
Chang WC (1991) Bamboos. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol. 16. Springer, Berlin, pp 211–237
Das P, Rout GR, Das AB (1993) Somatic embryogenesis in callus cultures of Mussaenda erythrophylla cvs. Plant Cell Tissue Organ Cult 35:199–201
Fujimura T, Komamine A (1980) Mode of action of 2,4-D and zeatin on somatic embryogenesis in carrot suspension culture. Z Pflazenphysiol 99:1–8
Heller J (1996) Physic nut, Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. No 1. International Plant Genetic Resource Institute, Rome
Litz RE, Gray DJ (1995) Somatic embryogenesis for agricultural improvement. World J Microbiol Biotechnol 11:416–425
Lo Schiavo F (1989) DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. Theor Appl Genet 77:325–331
Martin KP (2003) Plant regeneration through direct somatic embryogenesis on seed coat explants of cashew (Anacardium occidentale L.). Sci Hortic 98:299–304
Michaux-Ferriere N, Grout H, Carron M P (1992) Origin and ontogenesis of somatic embryos in Hevea brasiliensis (Euphorbiaceae). Am J Bot 79:174–180
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Neuenschwander B, Baumann TW (1992) A novel type of somatic embryogenesis in Coffea arabica. Plant Cell Rep 10:608–612
Openshaw K (2000) A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass Bioenerg 19:1–15
Prabakaran AJ, Sujatha M (1999) Jatropha tanjorensis Ellis and Saroja, a natural interspecific hybrid occurring in Tamil Nadu, India. Genet Resour Crop Evol 46:23–218
Raemakers CJJM, Jacobsen E, Visser RGF (1995) Secondary somatic embryogenesis and applications in plant breeding. Euphytica 81:93–107
Raemakers K, Jacobsen E, Visser R (2000) The use of somatic embryogenesis for plant propagation in Cassava. Mol Biotech 14:215–221
Rajore S, Batra A (2005) Efficient plant regeneration via shoot tip explant in Jatropha curcas. J Plant Biochem Biotech 14:73–75
Sardana J, Batra A, Ali DJ (2000) An expeditious method for regeneration of somatic embryos in Jatropha curcas L. Phytomorphology 50:239–242
Sharma AK, Sharma A (1990) Study of plant chromosomes from tissue culture. Chromosomal techniques theory and practice, 3rd edn. Aditya Books, pp 317–338
Sokal R, Rohlf FJ (1987) Introduction to Biostatistics, 2nd edn. Freeman WH, New York
Sujatha M, Mukta N (1996) Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tissue Organ Cult 44:135–141
Sujatha M, Makkar HPS, Becker K (2006) Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Reg 47:83–90
Wei Q, Lu WD, Loao Y, Pan SL, Xu Y, Tang L, Chen Fang (2004) Plant regeneration from epicotyl explants of Jatropha curcas. J Plant Physiol Mol Biol 30:475–478
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Financial assistance from the Department of Botany, Presidency College, Kolkata is gratefully acknowledged.
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Jha, T.b., Mukherjee, P. & Datta, M.M. Somatic embryogenesis in Jatropha curcas Linn., an important biofuel plant. Plant Biotechnol Rep 1, 135–140 (2007). https://doi.org/10.1007/s11816-007-0027-2
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DOI: https://doi.org/10.1007/s11816-007-0027-2