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Whole genome amplification from plant cell colonies of somatic hybrids using strand displacement amplification

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Abstract

The application of strand displacement amplification (SDA) is demonstrated for whole genome amplification from nanograms to micrograms for DNA isolated from small plant cell colonies. Secondary digest amplified fragment length polymorphism (SD-AFLP) analysis confirmed that the amplified genome is a representative of the entire genome. This approach allows the amplification of DNA isolated from small cell colonies of putative somatic hybrids for rapid molecular confirmation of the hybrid status of fusion products.

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Acknowledgments

The authors are grateful to Sylvia K. Erasmuson and Andrew S. Catanach for expert technical assistance in SDAFLP.

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Authors and Affiliations

  1. National Centre for Advanced Bio-Protection Technologies, Lincoln University, PO Box 84, Canterbury, New Zealand

    S. V. Raikar, A. J. Conner & M. C. Christey

  2. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand

    S. V. Raikar, R. Braun, A. J. Conner & M. C. Christey

  3. Pastoral Genomics, PO Box 109-185, Newmarket, Auckland, New Zealand

    C. Bryant

Authors
  1. S. V. Raikar
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  2. C. Bryant
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  3. R. Braun
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  4. A. J. Conner
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  5. M. C. Christey
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Correspondence to S. V. Raikar.

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Raikar, S.V., Bryant, C., Braun, R. et al. Whole genome amplification from plant cell colonies of somatic hybrids using strand displacement amplification. Plant Biotechnol Rep 1, 175–177 (2007). https://doi.org/10.1007/s11816-007-0026-3

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  • DOI: https://doi.org/10.1007/s11816-007-0026-3

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