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Enhancement of salt tolerance in transgenic rice expressing an Escherichia coli catalase gene, katE

Plant Biotechnology Reports volume 1, pages 49–55 (2007)Cite this article

Abstract

Rice (Oryza sativa) is sensitive to salt stresses and cannot survive under low salt conditions, such as 50 mM NaCl. In an attempt to improve salt tolerance of rice, we introduced katE, a catalase gene of Escherichia coli, into japonica rice cultivar, Nipponbare. The resultant transgenic rice plants constitutively expressing katE were able to grow for more than 14 days in the presence of 250 mM NaCl, and were able to form flower and produce seeds in the presence of 100 mM NaCl. Catalase activity in the transgenic rice plants was 1.5- to 2.5-fold higher than non-transgenic rice plants. Our results clearly indicate that simple genetic modification of rice to express E. coli-derived catalase can efficiently increase its tolerance against salt stresses. The transformant presented here is one of the most salt-tolerant rice plants created by molecular breeding so far.

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Acknowledgments

We thank Mr. Masateru Yamanaka and Mr. Masataka Asada, Department of Agriculture, Tokyo University of Agriculture for technical advice and valuable comments. We also thank Dr. Munetaka Sugiyama, Graduate School of Science, The University of Tokyo for critical reading of the manuscript.

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Authors and Affiliations

  1. Tokyo University of Agriculture, 1737 Funako, Atsugi, 243-0034, Japan

    Kenji Nagamiya, Tsuyoshi Motohashi, Kimiko Nakao, Shamsul Haque Prodhan & Eriko Hattori

  2. National Institute of Agrobiological Science, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Sakiko Hirose, Kenjiro Ozawa & Yasunobu Ohkawa

  3. National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-8555, Japan

    Kenjiro Ozawa

  4. Graduate School of Bioagricultural Science, Nagoya University, Furoucho, Chikusa-ku, Nagoya, 646-0804, Japan

    Kenji Nagamiya & Tetsuko Takabe

  5. Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya, 468-8502, Japan

    Teruhiro Takabe

  6. The Research Institute of Evolutionary Biology, 2-4-28 Kamiyoga, Setagaya, Tokyo, 158-0098, Japan

    Atsushi Komamine

  7. Sugimura International Patent and Trade Mark Agency Bureau, Kazan Building 7th floor, 3-2-4 Kasumigaseki, Chiyoda-ku, Tokyo, 100-0013, Japan

    Kenji Nagamiya

Authors
  1. Kenji Nagamiya
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  2. Tsuyoshi Motohashi
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  3. Kimiko Nakao
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  4. Shamsul Haque Prodhan
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  5. Eriko Hattori
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  6. Sakiko Hirose
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  7. Kenjiro Ozawa
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  8. Yasunobu Ohkawa
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  9. Tetsuko Takabe
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  10. Teruhiro Takabe
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  11. Atsushi Komamine
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Correspondence to Kenji Nagamiya.

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Nagamiya, K., Motohashi, T., Nakao, K. et al. Enhancement of salt tolerance in transgenic rice expressing an Escherichia coli catalase gene, katE. Plant Biotechnol Rep 1, 49–55 (2007). https://doi.org/10.1007/s11816-007-0007-6

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  • DOI: https://doi.org/10.1007/s11816-007-0007-6

Keywords

  • Catalase
  • katE
  • Oryza sativa
  • Salt tolerance