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Optimization study for enhanced production of hyaluronic acid from Streptococcus equisimilus MK156140

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Abstract

Hyaluronic acid finds its complete application in areas such as therapeutics, cosmetics, and as a health supplement. In the present investigation, standardization for the production of hyaluronic acid by Streptococcus equisimilus MK156140 in complex media was performed. Some of the selected physicochemical parameters such as pH, temperature, speed, incubation time, sucrose, yeast extract, and beef extract were screened using Plackett-Burman foldover design. Further, the screened parameters interaction was investigated using central composite design (CCD) and closely compared with OVAT studies. At a pH of 7.38, with beef extract, 12.15%, and yeast extract 7.64%, the observed yield was 7.16 g/L, which was in close line with the predicted value of 7.21 g/L.

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Funding

This work was supported by “Rajiv Gandhi National Fellowship” — No: F1-17.1/2017-18/RGNF-2017-18-SC-KAR-46952.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bangalore, 560 056, India

    Ashwini Kumar & Savitha Janakiraman

  2. M.S. Ramaiah Institute of Technology, Bangalore, 560054, India

    Lokesh Kyathsandra Nataraj

Authors
  1. Ashwini Kumar
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  2. Savitha Janakiraman
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  3. Lokesh Kyathsandra Nataraj
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Contributions

Ashwini K: Planning of work, experimental design, compiling data and manuscript writing.

Savitha J: Provided value added guidance and manuscript editing.

Lokesh N: RSM Data analysis.

Corresponding author

Correspondence to Savitha Janakiraman.

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Conflict of Interest with Ethical Standards Authors declare that there is no conflict of interest. No animals or humans were used during the present study.

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Kumar, A., Janakiraman, S. & Nataraj, L.K. Optimization study for enhanced production of hyaluronic acid from Streptococcus equisimilus MK156140. Korean J. Chem. Eng. 38, 1880–1887 (2021). https://doi.org/10.1007/s11814-021-0798-0

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  • DOI: https://doi.org/10.1007/s11814-021-0798-0

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