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Improved biodegradation of polyvinyl alcohol by hybrid nanoflowers of degrading enzymes from Bacillus niacini

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Abstract

Polyvinyl alcohol (PVA) is a synthetic polymer that is difficult to degrade in nature. In this study, we synthesized PVA-degrading enzymes (PVAase)-Cu3(PO4)2 hybrid nanoflowers by using crude PVAase from Bacillus niacini for PVA degradation. The influences of PVAase concentration, Cu2+ concentration, and incubation time on the nucleation and activity of the PVAase hybrid nanoflower were investigated. The maximal activity recovery of the PVAase hybrid nanoflower was approximately 85% at 0.25 mg/mL of PVAase, 0.36mM Cu2+, and 72 h incubation time. The optimum temperature and pH of PVAase did not change before and after immobilization. Compared with free PVAase, the PVAase hybrid nanoflower showed high thermal stability and storage stability. Additionally, the PVAase hybrid nanoflower displayed excellent reusability after eight cycles and promising PVA degradability, indicating its potential application in PVA degradation.

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Acknowledgements

This work is partially supported by the Open Project Program of State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology (project no. SKLFNS-KF201904). Dr. J.D. Cui also thanks the support from the Key Projects of Tianjin Natural Science Foundation, China (project no. 19JCZDJC38100), and the Foundation (No. 19272809D) of Key R & D projects of science and Technology Department of Hebei Province.

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Authors and Affiliations

  1. Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhang, 050000, P. R. China

    Hongjie Bian & Mengfei Cao

  2. State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, No 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin, 300457, P. R. China

    Hongjie Bian, Gaoyang Wang, Ziyuan Wang & Jiandong Cui

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  1. Hongjie Bian
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  2. Gaoyang Wang
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  3. Mengfei Cao
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  4. Ziyuan Wang
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  5. Jiandong Cui
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Correspondence to Jiandong Cui.

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Bian, H., Wang, G., Cao, M. et al. Improved biodegradation of polyvinyl alcohol by hybrid nanoflowers of degrading enzymes from Bacillus niacini. Korean J. Chem. Eng. 37, 1020–1028 (2020). https://doi.org/10.1007/s11814-020-0547-9

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  • DOI: https://doi.org/10.1007/s11814-020-0547-9

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