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Korean Journal of Chemical Engineering

, Volume 31, Issue 3, pp 490–495 | Cite as

Isolation and characterization of thermostable phycocyanin from Galdieria sulphuraria

  • Myounghoon Moon
  • Sanjiv K. Mishra
  • Chul Woong Kim
  • William I. Suh
  • Min S. Park
  • Ji-Won YangEmail author
Biotechnology

Abstract

Phycocyanin is a highly valuable pigmented protein synthesized by several species of cyanobacteria and red alga. In this study we demonstrate the production of thermostable phycocyanin from the unicellular red alga Galdieria sulphuraria. Phycocyanin was extracted by repeated freeze-thaw cycles and purified in a two-step process using ammonium sulfate fractionation, at 25% and 50% concentrations. Purified phycocyanin exhibited maximum absorbance at 620 nm, and the purity ratio (A620/A280) was found to be greater than 4. The recovery efficiency of phycocyanin from the crude extract was above 80%. In total, approximately 19 milligram pure phycocyanin was obtained from 3 g of wet cell mass of Galdieria sp. Subunits α and β of the protein were separated by SDS-PAGE and analyzed by MALDITOF mass spectrometry for identification, which confirmed that the isolated protein is phycocyanin. The molecular weight of α and β subunits of phycocyanin was found to be 17.6 and 18.4 kDa, respectively.

Keywords

Galdieria sulphuraria Red Algae Value-added Product Phycocyanin Purification 

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Copyright information

© Korean Institute of Chemical Engineers, Seoul, Korea 2014

Authors and Affiliations

  • Myounghoon Moon
    • 1
  • Sanjiv K. Mishra
    • 2
  • Chul Woong Kim
    • 1
  • William I. Suh
    • 2
  • Min S. Park
    • 1
    • 2
  • Ji-Won Yang
    • 1
    • 2
    Email author
  1. 1.Department of Chemical and Biomolecular EngineeringKAISTDaejeonKorea
  2. 2.Advanced Biomass R&D Center, #2502 Building W1-3KAISTDaejeonKorea

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