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Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations

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Abstract

Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, Q 3 Si (OH) and Q 4 Si , which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment.

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Authors and Affiliations

  1. SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France

    Hassane Oudadesse, Eric Wers, Xuang Vuong Bui, Claire Roiland, Bruno Bureau, Imane Akhiyat, Amany Mostafa & Pascal Pellen-Mussi

  2. Faculté des Sciences et Techniques de Mohammedia (FSTM), Université Hassan II, Maroc, France

    Imane Akhiyat & Hassan Chaair

  3. Biomaterilas Department, National Research Centre (NRC), 12622, Dokki, Cairo, Egypt

    Amany Mostafa

  4. LISM-URCA, 21 rue Clément Ader, BP 138, 51685, Reims Cedex 02, France

    Hicham Benhayoune & Joel Fauré

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  1. Hassane Oudadesse
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  3. Xuang Vuong Bui
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Correspondence to Hassane Oudadesse.

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Oudadesse, H., Wers, E., Bui, X.V. et al. Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations. Korean J. Chem. Eng. 30, 1775–1783 (2013). https://doi.org/10.1007/s11814-013-0104-x

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  • DOI: https://doi.org/10.1007/s11814-013-0104-x

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