Abstract
A novel enzymatic extraction combined with response surface methodology was developed for polysaccharide extraction from marine algae. Box-Behnken design was employed to optimize concentration of enzyme, ratio of water to raw material and extraction time for high extraction yields. The optimal extraction conditions were as follows: a concentration of enzyme of 778.01 mg/g with ratio of water to raw material of 86.80 and extraction time of 129.93 min for Gelidium amansii and a concentration of enzyme of 997.03 mg/g with ratio of water to raw material of 98.76 and extraction time of 117.69 min for Laminaria japonica Aresch. Under the optimal conditions, extraction yields were 48.36% and 32.47%, respectively. Experimental data were fitted by multiple regression analysis to a secondorder polynomial equation and were statistically analyzed. The predicted model matched the experimental data well with coefficients of determination (R2) of 0.9864 and 0.9892, respectively.
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Li, S., Han, D. & Row, K.H. Optimization of enzymatic extraction of polysaccharides from some marine algae by response surface methodology. Korean J. Chem. Eng. 29, 650–656 (2012). https://doi.org/10.1007/s11814-011-0221-3
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DOI: https://doi.org/10.1007/s11814-011-0221-3