Abstract
The type III secretion system (T3SS) is a mechanism by which bacteria export proteins from the cytoplasm, through the membranes, to the extracellular environment. T3SS is made up of more than 20 different proteins, about half of which maintain conserved sequences. This review summarizes the features of this novel apparatus and discusses the potential of utilizing T3SS to export recombinant proteins into the external environment, and the impact this system will have on protein production technology.
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A. J. Blocker, J. E. Deane, A. K. J. Veenendaal, P. Roversi, J. L. Hodgkinson, S. Johnson and S. M. Lea, Proc. Natl. Acad. Sci. USA, 105, 6507 (2008).
T. C. Marlovits, T. Kubori, A. Sukhan, D. R. Thomas, J. E. Galán and V. M. Unger, Science, 306, 1040 (2004).
G. R. Cornelis, Nat. Rev. Micro., 4, 811 (2006).
S.Y. He, K. Nomura and T. S. Whittam, Biochim. Biophys. Acta., 1694, 181 (2004).
J. E. Galán and H. Wolf-Watz, Nature, 444, 567 (2006).
C. J. Hueck, Microbiol. Mol. Biol. Rev., 62, 379 (1998).
T. G. Kimbrough and S. I. Miller, Microb. Infect., 4, 75 (2002).
C. A. Lee, Trends Microbiol., 5, 148 (1997).
T. F. Moraes, T. Spreter and N. C. J. Strynadka, Curr. Op. in Str. Biol., 18, 258 (2008).
T. C. Marlovits, T. Kubori, M. Lara-Tejero, D. Thomas, V. M. Unger and J. E. Galan, Nature, 637 (2006).
A. Blocker, N. Jouihri, E. Larquet, P. Gounon, F. Ebel, C. Parsot, P. Sansonetti and A. Allaoui, Mol. Microbiol., 39, 652 (2001).
A. M. Crago and V. Koronakis, Mol. Microbiol., 30, 47 (1998).
S. Daefler and M. Russel, Mol. Microbiol., 28, 1367 (1998).
A. Blocker, K. Komoriya and Aizawa, Proc. Natl. Acad. Sci. USA, 3027 (2003).
C. M. Collazo and J. E. Galán, Infect. Immun., 64, 3524 (1996).
T. Kubori, A. Sukhan, S.-I. Aizawa and J. E. Galán, Proc. Natl. Acad. Sci. USA, 97, 10225 (2000).
R. Macnab, Biochim. Biophys. Acta, 207 (2004).
L. Journet, C. Agrain, P. Broz and G. R. Cornelis, Science, 302, 1757 (2003).
J. Magdalena, A. Hachani, M. Chamekh, N. Jouihri, P. Gounon, A. Blocker and A. Allaoui, J. Bacteriol., 184, 3433 (2002).
K. Tamano, S. I. Aizawa, E. Katayama, T. Nonaka, S. Imajoh-Ohmi, A. Kuwae, S. Nagai and C. Sasakawa, EMBO J., 19, 3876 (2000).
M. Espina, A. J. Olive, R. Kenjale, D. S. Moore, S. F. Ausar, R.W. Kaminski, E.V. Oaks, C. R. Middaugh, W. D. Picking and W. L. Picking, Infect. Immun., 74, 4391 (2006).
C. A. Mueller, P. Broz, S.A. Muller, P. Ringler, F. Erne-Brand, I. Sorg, M. Kuhn, A. Engel and G. R. Cornelis, Science, 310, 674 (2005).
M. Sani, A. Botteaux, C. Parsot, P. Sansonetti, E. J. Boekema and A. Allaoui, Biochim. Biophys. Acta, 1770, 307 (2007).
A.K. J. Veenendaal, J. L. Hodgkinson, L. Schwarzer, D. Stabat, S. F. Zenk and A. J. Blocker, Mol. Microbiol., 63, 1719 (2007).
R. K. Wilson, R. K. Shaw, S. Daniell, S. Knutton and G. Frankel, Cell Microbiol., 3, 753 (2001).
L. A. Wainwright and J. B. Kaper, Mol. Microbiol., 27, 1247 (1998).
S. Woestyn, M. P. Sory, A. Boland, O. Lequenne and G. R. Cornelis, Mol. Microbiol., 20, 1261 (1996).
C. E. Stebbins and J. E. Galán, Nat. Rev. Mol. Cell Bio., 738 (2003).
P. Ghosh, Microbiol. Mol. Biol. Rev., 68, 771 (2004).
S.A. Müller, C. Pozidis, R. Stone, C. Meesters, M. Chami, A. Engel, A. Economou and H. Stahlberg, Mol. Microbiol., 61, 119 (2006).
K. Schesser, E. Frithz-Lindstein and H. WolfWatz, J. Bacteriol., 178, 7227 (1996).
M. P. Sory, A. Boland, I. Lambermont and G. R. Cornelis, Proc. Natl. Acad. Sci. USA, 92, 11998 (1995).
V. Bajaj, R. L. Lucas, C. Hwang and C. A. Lee, Mol. Microbiol., 22, 703 (1996).
V. Bajaj, C. Hwang and C. A. Lee, Mol. Microbiol., 18, 715 (1995).
K. Kaniga, J. C. Bossio and J. E. Galán, Mol. Microbiol., 13, 555 (1994).
C. Johnston, D.A. Pegues, C. J. Hueck, C. A. Lee and S. I. Miller, Mol. Microbiol., 22, 715 (1996).
I. Behlau and S. I. Miller, J. Bacteriol, 175, 4475 (1993).
S. I. Miller, A. M. Kukral and J. J. Mekalanos, Proc. Natl. Acad. Sci. USA, 86, 5054 (1989).
A. Economou and W. Wickner, Cell, 78, 835 (1994).
M. Pohlschroder, E. Hartmann, N. J. Hand, K. Dilks and A. Haddad, Annu. Rev. Microbiol., 91 (2005).
M. DeLisa, D. Tullman and G. Georgiou, Proc. Natl. Acad. Sci. USA, 100, 6115 (2003).
F. Sargent, E.G. Bogsch, N. R. Stanley, M. Wexler, C. Robinson, B. C. Berks and T. Palmer, EMBO J., 3640 (1998).
A. Rodrigue, A. Chanal, K. Beck, M. Muller and L. F. Wu, J. Biol. Chem., 274 13223 (1999).
. Wickner and R. Schekman, Science, 1452 (2005).
B. C. Berks, Mol. Microbiol., 22, 393 (1996).
H. Nielsen, J. Engelbrecht, S. Brunak and G. VonHeijne, Protein Eng., 10, 1 (1997).
T. Morita-Ishihara, O. Michinaga, S. Hiroshi, Y. Mitutaka, K. Eisaku and S. Chihiro, J. Biol. Chem., 281, 599 (2006).
A. O. Charkowski, H. C. Huang and A. Collmer, J. Bacteriol., 179, 3866 (1997).
B. B. Finlay and S. Falkow, Microbiol. Mol. Biol. Rev., 61, 136 (1997).
S. C. Winans, D. L. Burns and P. J. Christie, Trends Microbiol., 4, 64 (1996).
D. M. Widmaier, D. Tullman-Ercek, E. A. Mirsky, R. Hill, S. Govindarajan, J. Minshull and C. A. Voigt, Mol. Syst. Biol., 5, 309 (2009).
C. Altier, J. Microbiol., 43, 85 (2005).
M. F. Feldman, S. Müller, E. Wüest and G. R. Cornelis, Mol. Microbiol., 46, 1183 (2002).
J. E. Galán and R. I. Curtiss, Proc. Natl. Acad. Sci. USA, 86, 6383 (1989).
H. Russmann, H. Shams, F. Poblete, Y. Fu, J. E. Galan and R. O. Donis, Science, 281, 565 (1998).
A. P. Boyd, N. Grosdent, S. Totemeyer, C. Geuijen, S. Bleves, M. Iriartes, I. Lambermont, J. N. Octave and G. R. Cornelis, Eur. J. Cell Biol., 79, 659 (2000).
V. Konjufca, S. Y. Wanda, M. C. Jenkins and R. Curtiss, Infect. Immun., 74, 6785 (2006).
T. Kubori, Y. Matsushima, D. Nakamura, J. Uralil, M. Lara-Tejero, A. Sukhan, J. E. Galan and S.-I. Aizawa, Science, 280, 602 (1998).
T. Kubori and J. E. Galan, J. Bacteriol., 184, 4699 (2002).
K. Majander, L. Anton, J. Antikainen, H. Lang, M. Brummer, T.K. Korhonen and B. Westerlund-Wikstrom, Nat. Biotechnol., 23, 475 (2005).
Y. Wang, A. N. Ouellette, C.W. Egan, T. Rathinavelan, W. Im and R. N. De Guzman, J. Mol. Biology, 371, 1304 (2007).
P. Aldridge, J. Karlinsey and K.T. Hughes, Mol. Microbiol., 49, 1333 (2003).
J. C. Q. Bennett, J. Thomas, G. M. Fraser and C. Hughes, Mol. Microbiol., 39, 781 (2001).
F. Auvray, J. Thomas, G. M. Fraser and C. Hughes, J. Mol. Biology, 308, 221 (2001).
G. M. Fraser, J. C. Q. Bennett and C. Hughes, Mol. Microbiol., 32, 569 (1999).
P. A. Bronstein, E. A. Miao and S. I. Miller, J. Bacteriol., 182, 6638 (2000).
K. H. Darwin and V. L. Miller, EMBO J., 20, 1850 (2001).
S. C. Tucker and J. E. Galan, J. Bacteriol., 182, 2262 (2000).
C. E. Stebbins and J. E. Galan, Nature, 414, 77 (2001).
K. H. Darwin, L. S. Robinson and V. L. Miller, J. Bacteriol., 183, 1452 (2001).
J. Ruiz-Albert, R. Mundy, X. J. Yu, C. R. Beuzon and D.W. Holden, Microbiology, 149, 1103 (2003).
O. Schraidt, M. D. Lefebre, M. J. Brunner, W. H. Schm, A. Schmidt, R. Julia, K. Mechtler, J. E. Galán and T. C. Marlovits, PLoS Pathogens, 6 (2010).
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Vo, L.T.D., Hong, S.H. Recombinant protein secretion via the type III secretion system. Korean J. Chem. Eng. 28, 1573–1579 (2011). https://doi.org/10.1007/s11814-011-0024-6
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DOI: https://doi.org/10.1007/s11814-011-0024-6