Skip to main content
SpringerLink
Log in

Two-step bioprocess employing whole cell and enzyme for economical biodiesel production

  • Published:
Korean Journal of Chemical Engineering Aims and scope Submit manuscript

Abstract

A preliminary research for feasible and economical bioprocess for biodiesel production was carried out. A microorganism producing lipase was isolated from grease-contaminated soil. After chemically mutating twice, the microorganism exhibited 2.5 times higher intracellular lipase activities than wild type one and was used as a whole cell biocatalyst for biodiesel production. 0.1% toluene treatment followed by freeze drying was the optimum preparation method for whole cell biocatalyst. 1 g whole cell biocatalyst corresponded to 0.15 g commercial enzyme under mild methanol inhibition condition, while corresponding to 0.7 g enzyme under severe methanol inhibition environment. A two-step process, biodiesel production by the whole cell and sequentially by commercial enzyme, was suggested. This novel process, combining the advantages of whole cell and enzyme, saved the usage of commercial enzyme and alleviated enzyme deactivation by methanol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Y. K. Hong and W.H. Hong, Korean Chem. Eng. Res., 45(5), 424 (2007).

    CAS  Google Scholar 

  2. D. Antoni, V.V. Zverlow and W.H. Schwarz, Appl. Microbiol. Biotechnol., 77, 23 (2007).

    Article  CAS  Google Scholar 

  3. D. H. Lee, J.M. Kim, H.Y. Shin, S.W. Kang and S.W. Kim, Biotechnol. Bioprocess Eng., 11(6), 522 (2006).

    Article  CAS  Google Scholar 

  4. H. Fukuda, S. Hama, S. Tamalampudi and H. Noda, Trends in Biotechnol., 26(12), 668 (2008).

    Article  CAS  Google Scholar 

  5. K. Ban, S. Hama, K. Nishizuka, M. Kaieda, T. Matsumoto, A. Kondo, H. Noda and H. Fukuda, J. Molecular Catal. B: Enzymatic, 17, 157 (2002).

    Article  CAS  Google Scholar 

  6. S. A. Jun, C. H. Kang, S.W. Kong, B. I. Sang and Y. S. Um, Korean J. Biotechnol. Bioeng., 23(5), 413 (2008).

    Google Scholar 

  7. L. Li, W. Du, L. Wang and Z. Li, J. Molecular Catal. B: Enzymatic, 43, 58 (2006).

    Article  CAS  Google Scholar 

  8. Y. Shimada, Y. Waanabe, T. Samukawa, A. Sugihara, H. Noda, H. Fukuda and Y. Tominaga, JAOCS, 76, 789 (1999).

    Article  CAS  Google Scholar 

  9. Y. Shimada, Y. Watanabe, A. Suglihara and Y. J. Molecular Catal. B: Enzymatic, 17, 133 (2002).

    Article  CAS  Google Scholar 

  10. Y. Watanabe, Y. Shimada, A. Sugihara, H. Noda, H. Fukuda and Y. Tominaga, J. Am. Oil. Chem. Soc., 77, 355 (2000).

    Article  CAS  Google Scholar 

  11. V. R. Srivathsan, L. N. Srinivasan and M. Karuppan, Bioresource Technol., 99, 3975 (2008).

    Article  Google Scholar 

  12. T. Samukawa, M. Kaieda, T. Matsumoto, K. Ban, A. Kondo. Y. Shimada, H. Noda and H. Fukuda, J. Bioresour. Bioeng., 90, 180 (2000).

    CAS  Google Scholar 

  13. L. Li, W. Du, D. Liu, L. Wang and Z. Li, J. Molecular Catal. B: Enzymatic, 43, 58 (2006).

    Article  CAS  Google Scholar 

  14. K. Ban, M. Kaieda, T. Matsumoto, A. Kondo and H. Fukuda, Biochem. Eng., 8, 39 (2001).

    Article  CAS  Google Scholar 

  15. S. Hama, H. Yamaji, T. Fukumizu, T. Numata, S. Tamalampudi, A. Kondo, H. Nodac and H. Fukuda, Biochem. Eng. J., 34, 273 (2007).

    Article  CAS  Google Scholar 

  16. S. Tamalampudi, M. R. Talukder, S. Hama, T. Numata, A. Kondo and H. Fukuda, Biochem. Eng. J., 39(1), 185 (2008).

    Article  CAS  Google Scholar 

  17. W. Li, W. Du and D. Liu, Process Biochem., 42, 1481 (2007).

    Article  CAS  Google Scholar 

  18. T. Matsumoto, S. Takahashi, M. Kaieda, M. Ueda, A. Tanaka, H. Fukuda and A. Kondo, Appl. Microbiol. Biotechnol., 57, 515 (2001).

    Article  CAS  Google Scholar 

  19. W. Du, W. Li, T. Sun, X. Chen and D. Lin, Appl. Microbiol. Biotechnol., 79, 331 (2008).

    Article  CAS  Google Scholar 

  20. K. E. Jaeger, S. Ransac, B.W. Dijkstra, C. Colson, M. Vanheuvel and O. Misset, FEMS Microbiol. Rev., 15, 29 (1994).

    Article  CAS  Google Scholar 

  21. D.W. Lee, Y. S. Koh, K. J. Kim, B.C. Kim, H. J. Choi, D. S. Kim, M. T. Suhartono and Y. R. Ryun, FEMS Micobiol. Lett., 179, 393 (1999).

    Article  CAS  Google Scholar 

  22. T. C. Hung, R. Giridgar, S.H. Chiou and W. T. Wu, J. Mol. Catal. B: Enzym., 26, 69 (2003).

    Article  CAS  Google Scholar 

  23. A.W. Schwab, M. O. Bagby and B. Freedman, Fuel, 66, 1372 (1987).

    Article  CAS  Google Scholar 

  24. J.C. Chae, C.K. Kim and J. Z. Gerben, Biochem. Biophy. Res. Commun., 338, 1600 (2005).

    Article  CAS  Google Scholar 

  25. L. Yu, Y. Xu, X. Wang and X. Yu, J. Molecular Catal. B: Enzymatic, 47, 149 (2007).

    Article  CAS  Google Scholar 

  26. M.V. Flores, C. E. Voget and R. J. Ertola, J. Chem. Thehnol. Biotechnol., 64(4), 353 (1995).

    Article  CAS  Google Scholar 

  27. D. J. Jeon and S.H. Yeom, Bioprocess Biosys. Eng., Submitted (2010).

Download references

Author information

Authors and Affiliations

  1. Department of Environmental and Applied Chemical Engineering, Gangneung-Wonju National University, Gangneung, Gangwon-do, 210-702, Korea

    Dong Jin Jeon & Sung Ho Yeom

Authors
  1. Dong Jin Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sung Ho Yeom
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sung Ho Yeom.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jeon, D.J., Yeom, S.H. Two-step bioprocess employing whole cell and enzyme for economical biodiesel production. Korean J. Chem. Eng. 27, 1555–1559 (2010). https://doi.org/10.1007/s11814-010-0263-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11814-010-0263-y

Key words

Search

Navigation