Skip to main content
SpringerLink
Log in

Optimization of culture media for Bacillus species by statistical experimental design methods

  • Biotechnology
  • Published:
Korean Journal of Chemical Engineering Aims and scope Submit manuscript

Abstract

The medium compositions of Bacillus species (three strains) were optimized sequentially through two statistical experimental designs, Plackett-Burman and the Box-Behnken, which were determined by representative three-factor response-surface methodology. pH and DO control was found to enhance the viable cell number of Bacilli. Most strains grew well in the optimum condition and reached nearly up to of 5×109 CFU/mL. Additionally, when three of the Bacilli were cultivated in the optimized medium with pH and DO control, the viable cell number increased 3.6 times in B. subtilis, 64.1 times in B. licheniformis and 8.3 times in B. coagulans, respectively, in comparison with the original industrial medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. B. Fuller, J. Appl. Bacteriol., 66, 365 (1989).

    CAS  Google Scholar 

  2. H. W. Smith, Nat., 258, 628 (1975).

    Article  CAS  Google Scholar 

  3. D. S. Parker, Ani. Nutr. Health, 29, 4 (1974).

    Google Scholar 

  4. D. C. Savage, Ann. Rev. Nutr., 6, 155 (1886).

    Article  Google Scholar 

  5. H. I. Veld, J. H. J. and R. Havenaar, J. Chem. Technol. Biotechnol., 51, 562 (1991).

    Google Scholar 

  6. T. McCann, T. Egan and G. H. Weber, J. Food Protect, 59, 41 (1996).

    Google Scholar 

  7. B. L. Liu and Y. M. Tzeng, Biopro. Eng., 18, 413 (1998).

    CAS  Google Scholar 

  8. L. Chan, J. Bai, Z. Cai and F. Ouyang, J. Biotechnol., 93, 27 (2002).

    Article  Google Scholar 

  9. S. Krishnan, S. G. Prapulla, D. Rajalakshmi, M. C. Misra and N. G. Karanth, Biopro. Eng., 19, 61 (1998).

    Article  CAS  Google Scholar 

  10. P. L. Pham, P. Taillandier, M. Delmas and P. Strehaiano, World J. Microbiol. Biotechnol., 14, 185 (1998).

    Article  CAS  Google Scholar 

  11. D. L. Beres and D. M. Hawkins, Ecol. Mod., 141, 171 (2001).

    Article  Google Scholar 

  12. K. Sunitha, J. K. Lee and T. K. Oh, Biopro. Eng., 21, 477 (1999).

    CAS  Google Scholar 

  13. K. Park and K. F. Reardon, Biotechnol. Lett., 18, 737 (1996).

    Article  CAS  Google Scholar 

  14. J. Philippe, C. Hbid, J. Destain, H. Razafindralambo, M. Paquot, E. D. Pauw and P. Thonart, Biochem. Biotechnol., 77, 223 (1999).

    Article  Google Scholar 

  15. I. Y. Lee, M. K. Kim, J. H. Lee, W. T. Seo, H. W. Lee and Y. H. Park, Biopro. Eng., 20, 283 (1999).

    CAS  Google Scholar 

  16. Ö. Küçük, Korean J. Chem. Eng., 23, 21 (2006).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Jae-Hee Cho, Young-Bum Kim & Eun-Ki Kim

Authors
  1. Jae-Hee Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young-Bum Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eun-Ki Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eun-Ki Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cho, JH., Kim, YB. & Kim, EK. Optimization of culture media for Bacillus species by statistical experimental design methods. Korean J. Chem. Eng. 26, 754–759 (2009). https://doi.org/10.1007/s11814-009-0126-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11814-009-0126-6

Key words

Search

Navigation