Abstract
A molecular-thermodynamic framework is proposed to describe protein precipitation by inorganic salt. The equation of state consists of a hard-sphere reference contribution and a perturbation term. The reference term is derived based on the modified Chiew’s model to describe the pre-aggregation effect of protein at various solution pH. In this study, we discuss protein-protein effective two-body potentials. The distribution and magnitude of charges on the surface of a protein vary significantly with pH. It changes the magnitude of charge-charge repulsion, charge-dipole attraction, dipole-dipole attraction, and induced dipole-induced dipole attraction forces between protein pairs in solution. The distribution of the charge fluctuation is slightly effective in solution pH. To investigate the effect of pH, modified charge fluctuation distribution model is proposed. Using the proposed model, we successfully describe the pH dependence of the protein precipitation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
P. A. Albertsson, Partition of cell particles and macromolecules, Wiley, New York (1986).
S. Askura and F. Oosawa, J. Poly. Sci., 33, 183 (1958).
S. Asakura and F. Oosawa, J. Chem. Phys., 22, 1255 (1954).
Y. C. Chiew, Molec. Phys., 70, 129 (1990).
C. J. Coen, H.W. Blanch and J.M. Prausnitz, AIChE J., 41, 1 (1995).
De H. Hek and A. Vrij, J. Colloid Interf. Sci., 41, 996 (1995).
P. R. Foster, P. Dunhill and M. D. Lilly, Biochem. Biophys. Acta., 317, 505 (1975).
A. P. Gast, C. K. Hall and W.G. Russel, J. Colloid Interf. Sci., 96, 251 (1983).
A. P. Gast, C. K. Hall and W.G. Russel, J. Farad. Discuss. Chem. Soc., 76, 189 (1983).
M. J. Grimson, J. Chem. Soc. Farad. Trans., 79(2), 817 (1983).
R. N. Haire, W. A. Tisel, J. C. White and A. Rosenberg, Biopolymers, 23, 2761 (1984).
H. C. Hamaker, Physica IV, 10, 1058 (1937).
J. G. Kirkwood and Shumaker, J. B. Proc. Natl. Acad. Sci., 38, 863 (1952).
D. Kuehner, H.W. Blanch and J. M. Prausnitz, Fluid Phase Equilibria, 116, 140 (1996).
D. Kuehner, C. Heyer, C. Ramsch, U. M. Fornefeld, H.W. Blanch and J. M. Prausnitz, Biophysical J., 73, 3211 (1997).
H. Mahadevan and C. K. Hall, AIChE J., 36, 1517 (1990).
L. A. Moreira, M. Bostrom, B. W. Ninham, E. C. Biscaia and F.W. Tavares, Colloids and Surfaces A: Physicochem. Eng. Aspects, 282, 457 (2006).
M. Q. Niederauer and C. E. Glatz, Adv. Biochem. Eng. Technol., 47, 159 (1992).
G. D. J. Phillies, J. Chem. Phys., 60, 2721 (1974).
F. Rothstein, in Protein precipitation process engineering, R. G. Harrion, ed., Dekker, New York (1994).
Y.-C. Shih, H.W. Blanch and J. M. Prausnitz, Biotech. Bioeng., 40, 1155 (1992).
F. W. Tavares and S. I. Sandler, AIChE J., 43, 218 (1997).
E. Verwey and J. Overbeek, Theory of stability of lyophobic colloids, Elsevier, Amsterdam (1948).
J. M. Victor and J. P. Hansen, J. Phys. Lett., 45, L–307 (1984).
V. Vlachy and J. M. Prausnitz, J. Phys. Chem., 96, 6465 (1992).
V. Vlachy, H.W. Blanch and J. M. Prausnitz, AIChE J., 39, 215 (1993).
A. Vrij, Pure and Appl. Chem., 48, 471 (1976).
J. Tao, J. S. Johansson and D.H. Haynes, Biochim Biophys Acta., 1105, 19 (1992).
H. Mahadevan and C. K. Hall, AIChE J., 38, 573 (1992).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, S.G., Bae, Y.C. & Kim, JJ. Effect of pH on phase separation of globular protein. Korean J. Chem. Eng. 26, 742–747 (2009). https://doi.org/10.1007/s11814-009-0124-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-009-0124-8