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Effects of moderate pressure on premeability and viability of Saccharomyces cerevisiae cells

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Abstract

With CO2 and N2 as the pressure media, the effects of the moderate pressure (0.1–1.0MPa) and the holding time on the conductivities of the cell suspension of Saccharomyces cerevisiae CICC1447 and Saccharomyces cerevisiae CICC1339, as well as the absorbances of the supernatant (after centrifuged) at 280 nm (A280) and 260 nm (A260) were determined. The membrane permeability of Saccharomyces cerevisiae CICC1447 increased significantly and the cell leakage was aggravated with the pressure increase. For Saccharomyces cerevisiae CICC1339, the conductivity of the cell suspension, A280 and A260 of the supernatant fluctuated with the pressure increase; as a whole, they increased with pressure. Different from high pressure, a moderate pressure not only remarkably improved the permeability of the yeast cell membrane, but also kept yeast cell viability; moreover, the integrity of the yeast cell membrane could be maintained.

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References

  1. J. C. Cheftel, Food Sci. Tech. Int., 1, 75 (1995).

    Article  Google Scholar 

  2. Y. Sh. Dong, Q. Zh. Yang and Sh. R. Jia, Biochem. Eng. J., 37, 226 (2007).

    Article  CAS  Google Scholar 

  3. M. A. Z. Coelho, I. Belo and R. Pinheiro, Appl. Microbiol. Biotechnol., 66, 318 (2004).

    Article  CAS  Google Scholar 

  4. C. E. Zobell and A. B. Cobet, J. Bacteriol., 87, 710 (1964).

    CAS  Google Scholar 

  5. P. Ma as and B. M. Mackey, Appl. Environ. Microbiol., 70, 1545 (2004).

    Article  Google Scholar 

  6. F. Abe, C. Kato and K. Horikoshi, Trends Microbiol., 7, 447 (1999).

    Article  CAS  Google Scholar 

  7. G. D. Bothun, B. L. Knutson and J. A. Berberich, Appl. Microbiol. Biotechnol., 65, 149 (2004).

    Article  CAS  Google Scholar 

  8. D. H. Bartlett, C. Kato and K. Horikoshi, Res. Microbiol., 146, 697 (1995).

    Article  CAS  Google Scholar 

  9. M. Summit, B. Scott and K. Nielso, Extremophiles, 2, 339 (1998).

    Article  CAS  Google Scholar 

  10. S. Brul, A. J. M. Rommens and C. T. Verrips, Innov. Food Sci. Emerging Technol., 1, 99 (2000).

    Article  CAS  Google Scholar 

  11. J. Y. Jung, T. Khan and J. K. Park, Korean J. Chem. Eng., 24, 1 (2007).

    Article  Google Scholar 

  12. M. Kato, R. Hayashi and T. Tsuda, Eur. J. Biochem., 269, 110 (2002).

    Article  CAS  Google Scholar 

  13. J. M. Perrier-Cornet, M. Hayert and P. Gervais, J. Appl. Microbiol., 87, 1 (1999).

    Article  CAS  Google Scholar 

  14. O. Friedrich, K. R. Kress and H. Ludwig, J. Membr. Biol., 188, 11 (2002).

    Article  CAS  Google Scholar 

  15. Zh. M. Ou, J. P. Wu, L. R. Yang and P. L. Cen, Korean J. Chem. Eng., 25, 124 (2008).

    Article  CAS  Google Scholar 

  16. J. H. Lee, Y. J. Yoo and K. H. Park, Korean J. Chem. Eng., 8, 39 (1991).

    Article  CAS  Google Scholar 

  17. P. M. B. Fernandes, A.D. Panek and E. Kurtenbach, FEMS Microbiol. Lett., 152, 17 (1997).

    Article  CAS  Google Scholar 

  18. H. Iwahashi, K. Obuchi and S. Fujii, FEBS Lett., 416, 1 (1997).

    Article  CAS  Google Scholar 

  19. M. A. Singer and S. Lindquist, Trends. Biotechnol., 16, 460 (1998).

    Article  CAS  Google Scholar 

  20. Y. Komatsu, K. Obuchi and H. Iwahashi, Biochem. Bioph. Res. Co., 174, 1141 (1991).

    Article  CAS  Google Scholar 

  21. Zh. H. Fan, Sh. R. Jia and S. C. Qiao, Chin. J. Biopro. Eng., 2, 36 (2004).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Industry Microbiology, Ministry of Education, Tianjin University of Science and Technology, 29 Thirteenth Street, Tai Da Development Area, Tianjin, 300457, P. R. China

    Shi-Ru Jia,  Na-Chen, Yu-Jie Dai, Chang-Sheng Qiao & Bo-Ning Liu

  2. Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang, 050018, P. R. China

    Jian-Dong Cui

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  1. Shi-Ru Jia
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  2. Na-Chen
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  3. Yu-Jie Dai
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  4. Chang-Sheng Qiao
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  5. Jian-Dong Cui
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  6. Bo-Ning Liu
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Correspondence to Shi-Ru Jia.

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The first author and the fifth author contributed to the work equally.

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Jia, SR., Na-Chen, Dai, YJ. et al. Effects of moderate pressure on premeability and viability of Saccharomyces cerevisiae cells. Korean J. Chem. Eng. 26, 731–735 (2009). https://doi.org/10.1007/s11814-009-0122-x

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  • DOI: https://doi.org/10.1007/s11814-009-0122-x

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