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Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920

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The development of a fermentation strategy for hyaluronic acid (HA) production by Streptococcus zooepidemicus ATCC 39920 has been explored. The specific HA productivity (Y P/X ) was affected by the medium carbon-tonitrogen (C/N) ratio rather than the specific growth rate of cells. Accordingly, HA fermentation should be performed in a balanced medium with an optimum C/N ratio of 2: 1 in a batch culture. To improve the performance of the batch culture, the operation conditions for the fill-and-draw culture were investigated. It was found that the timing of medium exchange is critical for successfully performing fill-and-draw operations. Since streptococcal cells at the stationary phase might lose the capacity of HA synthesis, the displacement of the medium in a fill-and-draw culture should be started at the late exponential growth phase.

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Correspondence to Shu-Jen Chen.

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Chen, SJ., Chen, JL., Huang, WC. et al. Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920. Korean J. Chem. Eng. 26, 428–432 (2009).

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