Abstract
This study reported simple surface modification for the immobilization of biomolecules such as proteins and cells onto desired area at micron-scale level. First, thin film composed of glycidyl methacrylate (GMA) was prepared by UV-photopolymerization. Then, the polyethylene glycol (PEG) microstructures which played a role in the prevention of nonspecific binding of biomolecules were fabricated by using micromolding in capillaries (MIMIC). Thus, we could easily obtain an orthogonal surface having biomolecular attraction and repulsion areas. In addition, we could control of the height of prepared PEG microstructures with spin coating or not. For the investigation of feasibility of biomolecule patterning onto the functionalized surface, FITC-BSA and HEK 293 were examined as representative biomolecule models. A functionalized surface with GMA promotes the strong adhesion of biomolecules, and PEG microstructures located on the background prevent nonspecific binding of biomolecules at micron-scale level. The orthogonal difference in surface functionality showed strong possibility of simple patterning of biomolecules. In addition, the proposed method could easily control the size, shape, and height of patterns. It will be useful platform technology for the construction of a biomolecule array.
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Song, HM., Lee, CS. Simple fabrication of functionalized surface with polyethylene glycol microstructure and glycidyl methacrylate moiety for the selective immobilization of proteins and cells. Korean J. Chem. Eng. 25, 1467–1472 (2008). https://doi.org/10.1007/s11814-008-0241-9
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DOI: https://doi.org/10.1007/s11814-008-0241-9