Korean Journal of Chemical Engineering

, Volume 25, Issue 5, pp 1131–1135 | Cite as

Characterization of thiosulfate-oxidizing Enterobacter hormaechei JH isolated from barnyard manure

  • Sung-Hyun Kim
  • In Hae Kim
  • Won Jae Lee
  • Jae-Hwa LeeEmail author


In an effort to discover sulfur-oxidizing microorganisms, a new bacterium was screened from barnyard manure. This strain was identified as Enterobacter hormaechei JH by its morphological, physiological, and biochemical properties, as well as by 16S rRNA gene sequence analysis. E. hormaechei JH are Gram-negative rod, non-spore-forming, and aerobic. The pH range for E. hormaechei JH growth was 2.0–9.0, and the optimal pH was determined as pH 7.0. The optimal temperature for growth was 30°C. We compared the growth of this strain with Thiobacillus delicates KCTC2851, which is a well known thiosulfate oxidizing microorganism. E. hormaechei JH presented greater growth than T. delicates KCTC2851. Thus, the above results indicate this strain is a candidate for improving the removal efficiency of biological deodorizing systems.

Key words

Sulfate-oxidation Enterobacter hormaechei JH Odor Removal Biological Deodorizing System 


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  1. 1.
    K. J. Oh, M. H. Seo, H. J. Son and D. G. Kim, Korean J. Chem. Eng., 15, 177 (1998).CrossRefGoogle Scholar
  2. 2.
    H. V. Langenhove, E. Wuyts and N. Schamp, Water Res., 20, 1471 (1986).CrossRefGoogle Scholar
  3. 3.
    Y. Yang and E. R. Allen, J. Air Waste Magm. Assoc., 44, 863 (1994).Google Scholar
  4. 4.
    G. E. Findlay and N. Nirmalakhandan, J. Air Waste Magm. Assoc., 34, 565 (1996).Google Scholar
  5. 5.
    E. Y. Lee, K. S. Cho and H. W. Ryu, Biotechnol. Biopress Eng., 5, 48 (2000).CrossRefGoogle Scholar
  6. 6.
    K. S. Cho, M. Hirai and M. Shoda, J. Ferment. Bioeng., 71, 384 (1991).CrossRefGoogle Scholar
  7. 7.
    Y. Tanji, T. Kanagawa and E. Mikami, J. Ferment. Bioeng., 67, 280 (1989).CrossRefGoogle Scholar
  8. 8.
    A. Wada, M. Shoda, H. Kubota, T. Kobayashi, Y. Katayama-Fujimura and H. Kuraishi, J. Ferment. Technol., 64, 161 (1986).CrossRefGoogle Scholar
  9. 9.
    D. J. Cork, R. Garunas and A. Sajjad, Appl. Environ. Microbiol., 45, 913 (1983).Google Scholar
  10. 10.
    Y. C. Chung, C. Huang and C. P. Tseng, Biotechnol. Tech., 10, 743 (1996).CrossRefGoogle Scholar
  11. 11.
    A, Subramaniyan, R. Kohatkar, K. L. Sublette and R. Beitle, Appl. Biochem. Biotechnol., 70, 995 (1998).CrossRefGoogle Scholar
  12. 12.
    P. F. Henshaw and W. Zhu, Water Res., 35, 3605 (2001).CrossRefGoogle Scholar
  13. 13.
    R. A. Deeb and L. Alvarex-Cohen, Biotechnol. Bioeng., 62, 526 (1999).CrossRefGoogle Scholar
  14. 14.
    S. K. Lee and S. B. Lee, J. Microbiol. Biotechnol., 12, 909 (2002).Google Scholar
  15. 15.
    K. J. Oh, K. C. Cho, Y. H. Chung, S. K. Park, S. K. Cho and D.G. Kim, Korean J. Chem. Eng., 23, 148 (2006).CrossRefGoogle Scholar
  16. 16.
    D. P. Kelly, J. K. Shergill, W. P. Lu and A. P. Antonie, Van Leeuwenhoek, 71, 95 (1997).CrossRefGoogle Scholar
  17. 17.
    A. P. Harrison, Annu. Rev. Microbiol., 38, 265 (1984).CrossRefGoogle Scholar
  18. 18.
    J. T. Pronk, R. Meulenberg, W. Hazeu, P. Bos and J. G. Kuenen, FEMS Microbiol. Rev., 7, 293 (1990).Google Scholar
  19. 19.
    D. E. Rawlings and T. Kusano, Microbiol. Rev., 58, 39 (1994).Google Scholar
  20. 20.
    D. C. Brune, Biochim. Biophys. Acta, 975, 189 (1989).CrossRefGoogle Scholar
  21. 21.
    H.G. Trûper and U. Fischer, Phil. Trans. R. Soc. Lond B, 298, 529 (2001).CrossRefGoogle Scholar
  22. 22.
    C. G. Friedrich, Adv. Microb.Physiol., 39, 235 (1998).CrossRefGoogle Scholar
  23. 23.
    D. Y. Sorokin, A. M. Lysenko, L. L. Mityushina, T. P. Tourova, B. E. Jones, F. A. Rainey, L. A. Robertson and J. G. Kuenen, Int. J. Syst. Evol. Microbiol., 51, 565 (2001).Google Scholar
  24. 24.
    J. Sambrook, E. R. Frisch and T. Maniatis, Molecular cloning: A laboratory manual, 2nd ed. Cold Springer Harbor. NY: Cold Springer Harbor Laboratory Press (1989).Google Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Sung-Hyun Kim
    • 1
  • In Hae Kim
    • 2
  • Won Jae Lee
    • 1
  • Jae-Hwa Lee
    • 2
    • 3
    Email author
  1. 1.Department of MicrobiologyPukyong National UniversityBusanKorea
  2. 2.Department of Pharmaceutical EngineeringCollege of Medical Life ScienceBusanKorea
  3. 3.Department of Bioscience and Biotechnology, College of EngineeringSilla UniversityBusanKorea

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