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Application of spent sulfidic caustics for autotrophic denitrification in a MLE process and their microbial characteristics by fluorescence in situ hybridization

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Abstract

Spent sulfidic caustics (SSCs) produced from petrochemical plants contain a high concentration of hydrogen sulfide and alkalinity, and some organic matter. Most of the SSCs are incinerated with the auxiliary fuel causing secondary pollution problems. The reuse of this waste is becoming increasingly important in terms of economical and environmental viewpoints. To denitrify wastewater with a low COD/N ratio, additional carbon sources are required. Therefore, autotrophic denitrification has received increasing attention. In this research, SSCs were injected as electron donors for sulfur-based autotrophic denitrification in a modified Ludzack-Ettinger (MLE) process. According to the variations in the SSCs dosage, the efficiencies of COD, nitrification and TN removal were evaluated. Heterotrophic denitrification by organic matter and autotrophic denitrification by SSCs were also investigated. As a result, adequate injection of SSCs showed stable autotrophic denitrification. To investigate some of the harmful effects of SSCs, fluorescence in situ hybridization (FISH) for nitrifying bacteria and Thiobacillus denitrificans was performed. Ammoniaoxidizing bacteria (AOB) and Nitrospira genus showed a similar pattern. Excessive injection of SSCs made nitrifying bacteria decrease and nitrification failure occur because of the high pH caused by the SSCs. The distribution of T. denitrificans was relatively uniform as SSCs were injected. This result means that T. denitrificans are available at high pH.

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References

  1. J. Surmacz-Gorska, A. Cichon and K. Miksch, Proceedings of the Env. Biotech., 1, 78 (1996).

    Google Scholar 

  2. T. C. Zhang and P. L. Bishop, Wat. Environ. Res., 68, 1107 (1996).

    Article  CAS  Google Scholar 

  3. H. D. Montieth, T. R. Bridle and P. M. Sutton, Evaluation of industrial waste carbon sources for biological denitrification, Environ. Canada Wastewater Tech. Centre Report, No. EPS 4-WP-79-9 (1979).

  4. N. Narcis, M. Rebhun and C. Scheindorf, Wat. Res., 13, 93 (1979).

    Article  Google Scholar 

  5. J. R. Skinde and S. K. Bhagat, J. WPCF, 54, 370 (1982).

    Google Scholar 

  6. T. J. Park, K. H. Lee, D. S. Kim and C.W. Kim, Wat. Sci. Tech., 34, 9 (1996).

    Article  CAS  Google Scholar 

  7. T. J. Park, K. H. Lee and J. H. Lee, Korean J. Chem. Eng., 15, 9 (1998).

    Article  Google Scholar 

  8. B. Batchelor and A.W. Lawrence, J. WPCF, 50, 1986 (1978).

    CAS  Google Scholar 

  9. G. Claus and H. J. Kutzner, Appl. Microbiol. Biotechnol., 22, 289 (1985).

    CAS  Google Scholar 

  10. L. Koenig and H. Liu, Wat. Sci. Tech., 34, 496 (1996).

    Article  Google Scholar 

  11. T. C. Zhang and D.G. Lampe, Wat. Res., 33, 599 (1999).

    Article  CAS  Google Scholar 

  12. S. E. Oh, Y. B. Yoo, J. C. Young and I. S. Kim, J. Biotech., 92, 1 (2001).

    Article  CAS  Google Scholar 

  13. J. P. van der Hock, W. A. Hijnen, C. A. van Bennekom and B. J. Mijnarends, J. Wat. SRT-Aqua, 41, 209 (1992).

    Google Scholar 

  14. J. P. van der Hock, J. W. N. M. Kappelhof and W. A. M. Hijen, J. Chem. Tech. Biotech., 54, 197 (1992).

    Google Scholar 

  15. J. P. van der Hock, J. W. N. M. Kappelhof and J. C. Schippers, J. Wat. SRT-Aqua, 43, 84 (1994).

    Google Scholar 

  16. J. M. Flere and T. C. Zhang, J. Environ. Eng., 8, 721 (1999).

    Google Scholar 

  17. S. H. Sheu and H. S. Weng, Wat. Res., 35, 2017 (2001).

    Article  CAS  Google Scholar 

  18. J. Sipma, A. Svitelskaya, B. van der Mark, L.W.H. Pol, G. Lettinga, C. J. N. Buisman and A. J. H. Janssen, Wat. Res., 38, 4331 (2004).

    Article  CAS  Google Scholar 

  19. I. G. Byun, J. H. Ko, Y. R. Jung, T. H. Lee, C. W. Kim and T. J. Park, Korean J. Chem. Eng., 22, 910 (2005).

    Article  CAS  Google Scholar 

  20. A. Schramm, D. De Beer, M. Wagner and R. Amann, Appl. Environ. Microbiol., 64, 3480 (1998).

    CAS  Google Scholar 

  21. A. Jang, P. L. Bishop, S. Okabe, S. G. Lee and I. S. Kim, Wat. Sci. Tech., 47, 49 (2002).

    Google Scholar 

  22. S. H. Hur, J. J. Park, Y. J. Kim, J. C. Yu, I. G. Byun, T. H. Lee and T. J. Park, Korean J. Chem. Eng., 24, 93 (2007).

    Article  CAS  Google Scholar 

  23. B. Sharma and R. C. Albert, Wat. Res., 11, 897 (1977).

    Article  CAS  Google Scholar 

  24. E. S. Choi and H. S. Lee, Korean J. Chem. Eng., 13, 364 (1996).

    Article  CAS  Google Scholar 

  25. W. Manz, M. Wagner, R. Amann and K. H. Schleifer, Wat. Res., 28, 1715 (1994).

    Article  CAS  Google Scholar 

  26. APHA, Standard methods for the examining of water and wastewater, 20th, American Public Health Association, Washington DC, USA (1998).

    Google Scholar 

  27. S. Villaverde, P. A. García-Encina and F. Fdz-Polanco, Wat. Res., 31, 1180 (1997).

    Article  CAS  Google Scholar 

  28. C. L. Laia and G. G. Jesus, J. Microbiol. Methods, 57, 69 (2004).

    Article  CAS  Google Scholar 

  29. H. Daims, A. Brühl, R. Amann, K. H. Schleifer and M. Wagner, System Appl. Microbiol., 22, 434 (1999).

    CAS  Google Scholar 

  30. B. K. Mobarry, M. Wagner, V. Urbain, E. Rittmann and D. A. Stahl, Appl. Environ. Microbiol., 62, 2156 (1996).

    CAS  Google Scholar 

  31. M. Wagner, G. Rath, H. P. Koops, J. Flood and R. Amann, Wat. Sci. Tech., 34(1/2), 237 (1996).

    Article  CAS  Google Scholar 

  32. H. Daims, P. H. Nielsen, J. L. Nielsen, S. Juretschko and M. Wagner, Wat. Sci. Tech., 41(4/5), 85 (2000).

    CAS  Google Scholar 

  33. T. Uki, Development of mRNA FISH by catalyed reporter deposition (CARD) methods targeting function gene of Aps in sulfate reducing bacteria, M.S.thesis, Department of environmental and urban engineering, Nagaoka national college of technology, Japan (2006).

    Google Scholar 

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Authors and Affiliations

  1. Department of Environmental Engineering, Pusan National University, Busan, 609-735, Korea

    Jeung-Jin Park, So-Ra Park, Dong-Jin Ju, Jeong-Keun An, Im-Gyu Byun & Tae-Joo Park

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  1. Jeung-Jin Park
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  2. So-Ra Park
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  3. Dong-Jin Ju
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Park, JJ., Park, SR., Ju, DJ. et al. Application of spent sulfidic caustics for autotrophic denitrification in a MLE process and their microbial characteristics by fluorescence in situ hybridization. Korean J. Chem. Eng. 25, 542–547 (2008). https://doi.org/10.1007/s11814-008-0091-5

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  • DOI: https://doi.org/10.1007/s11814-008-0091-5

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