The extract of Gynostemma pentaphyllum was tested to control the melanogenesis in B16 melanoma. Cytotoxic effect by the extract was observed when the dose concentration was higher than 2 mg/L. Most of the inhibitory effect was obtained by the reduced accumulation of extra-cellular melanin. When the extract was dosed as 2 mg/L, the extra-cellular melanin produced was only 24% of the control. Proteome analysis with 2-D PAGE showed that various proteins involved in melanogenesis were down-regulated by Gynostemma pentaphyllum. In addition to other proteins related to the intra-cellular melanogenesis, Rab-27b and Rab-38 could explain the remarkable decrease in extra-cellular melanin accumulation by reduced melanin transfer to keratinocyte.
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Lee, H.I., Yoo, B.S., Yoo, M.A. et al. Inhibition of melanogenesis and melanin transportation by Gynostemma pentaphyllum . Korean J. Chem. Eng. 24, 655–659 (2007). https://doi.org/10.1007/s11814-007-0020-z
- Gynostemma pentaphyllum
- 2-D PAGE