The extract of Gynostemma pentaphyllum was tested to control the melanogenesis in B16 melanoma. Cytotoxic effect by the extract was observed when the dose concentration was higher than 2 mg/L. Most of the inhibitory effect was obtained by the reduced accumulation of extra-cellular melanin. When the extract was dosed as 2 mg/L, the extra-cellular melanin produced was only 24% of the control. Proteome analysis with 2-D PAGE showed that various proteins involved in melanogenesis were down-regulated by Gynostemma pentaphyllum. In addition to other proteins related to the intra-cellular melanogenesis, Rab-27b and Rab-38 could explain the remarkable decrease in extra-cellular melanin accumulation by reduced melanin transfer to keratinocyte.
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B. Venkatesha, Y. Feng, K. Tsuneto, H. Youichiro, Y. Ken-Ichi, V. Julio, M. Jacqueline, D. V. Wilfred, W. Hidenori, S. Jeffrey, J. H. Vincent, F. H. Donald and A. Ettore, J. Proteome Research, 2, 69 (2003).
M. Jimbow, H. Kanoh and K. Jimbow, J. Invest Dermatol, 79, 97 (1982).
S. S. Shola and E. K. Barbara, Veterinary Dermatology, 14, 57 (2003).
G. Prota, J. Invest Dermal, 75, 122 (1980).
A. J. Sober and T. B. Fitzpatrick, Prog. Chem. Org. Natural Products, 31, 521 (1974).
B. Karine, L. Elizabeth, L. F. James, G. S. Yiqun, A. Gretchen, P. Kirsi, D. E. Allen, F. Kenji, A. N. David, G. A. Natalie and A. R. Katheryn, Cancer research, 63, 6716 (2003).
R. N. Valentina, T. W. H. Huang, V. H. Tran, G. Q. Li, C. Duke and B. D. Roufogalis, Phytochemistry Reviews, 4, 197 (2005).
H. L. Liu, T. H. Kao and B. H. Chen, Chromatographia, 60, 411 (2004).
G. W. Lee and S. Y. Byun, Biotechnol. Bioprocess Eng., 8, 299 (2003).
A. M. Sieuwerts, J. G. M. Klijkn, H. A. Peters and J. A. Foekens, Eur. J. Clin. Chem. Clin. Biochem., 33, 813 (1995).
J. Hosoi, E. Abe, T. Suda and T. Kuroki, Cancer Res., 45, 1474 (1985).
M. M. Bradford, Anal. Biochemistry, 72, 248 (1976).
A. Görg, C. Obermaier, G. Boguth, A. Harder, B. Scheibe, R. Wildgruber and W. Weiss, Electrophoresis, 21, 1037 (2000).
G. W. Lee, H. C. Yoon and S. Y. Byun, Enzyme and Microbial Technology, 35, 632 (2004).
B. B. Fuller, D. T. Spaulding and D. R. Smith, Exp. Cell Res., 262, 197 (2001).
N. Puri, J. M. Gardner and M. H. Brilliant, J. Invest. Dermatol., 115, 607 (2000).
K. Kameyama, T. Takemura, Y. Hamada, C. Sakai, S. Kondoh, S. Nishiyama, K. Urabe and V. J. Hearing, J. Invest. Dermatol., 100, 126 (1993).
D. Graphodatskaya, H. Joerg and G. Stranzinger, J. Recept. Signal Transduct Res., 22, 421 (2002).
S. De Schepper, J. M. Boucneau, W. Westbroek, M. Mommaas, J. Onderwater, L. Messiaen, J. M. Naeyaert and J. L. Lambert, J. Invest. Dermatol., 126, 635 (2006).
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Lee, H.I., Yoo, B.S., Yoo, M.A. et al. Inhibition of melanogenesis and melanin transportation by Gynostemma pentaphyllum . Korean J. Chem. Eng. 24, 655–659 (2007). https://doi.org/10.1007/s11814-007-0020-z