Korean Journal of Chemical Engineering

, Volume 24, Issue 4, pp 655–659 | Cite as

Inhibition of melanogenesis and melanin transportation by Gynostemma pentaphyllum

  • Hye In Lee
  • Byoung Sam Yoo
  • Mi Ae Yoo
  • Sang Yo ByunEmail author


The extract of Gynostemma pentaphyllum was tested to control the melanogenesis in B16 melanoma. Cytotoxic effect by the extract was observed when the dose concentration was higher than 2 mg/L. Most of the inhibitory effect was obtained by the reduced accumulation of extra-cellular melanin. When the extract was dosed as 2 mg/L, the extra-cellular melanin produced was only 24% of the control. Proteome analysis with 2-D PAGE showed that various proteins involved in melanogenesis were down-regulated by Gynostemma pentaphyllum. In addition to other proteins related to the intra-cellular melanogenesis, Rab-27b and Rab-38 could explain the remarkable decrease in extra-cellular melanin accumulation by reduced melanin transfer to keratinocyte.

Key words

Gynostemma pentaphyllum Melanogenesis Inhibition 2-D PAGE 


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  1. 1.
    B. Venkatesha, Y. Feng, K. Tsuneto, H. Youichiro, Y. Ken-Ichi, V. Julio, M. Jacqueline, D. V. Wilfred, W. Hidenori, S. Jeffrey, J. H. Vincent, F. H. Donald and A. Ettore, J. Proteome Research, 2, 69 (2003).CrossRefGoogle Scholar
  2. 2.
    M. Jimbow, H. Kanoh and K. Jimbow, J. Invest Dermatol, 79, 97 (1982).CrossRefGoogle Scholar
  3. 3.
    S. S. Shola and E. K. Barbara, Veterinary Dermatology, 14, 57 (2003).CrossRefGoogle Scholar
  4. 4.
    G. Prota, J. Invest Dermal, 75, 122 (1980).CrossRefGoogle Scholar
  5. 5.
    A. J. Sober and T. B. Fitzpatrick, Prog. Chem. Org. Natural Products, 31, 521 (1974).Google Scholar
  6. 6.
    B. Karine, L. Elizabeth, L. F. James, G. S. Yiqun, A. Gretchen, P. Kirsi, D. E. Allen, F. Kenji, A. N. David, G. A. Natalie and A. R. Katheryn, Cancer research, 63, 6716 (2003).Google Scholar
  7. 7.
    R. N. Valentina, T. W. H. Huang, V. H. Tran, G. Q. Li, C. Duke and B. D. Roufogalis, Phytochemistry Reviews, 4, 197 (2005).CrossRefGoogle Scholar
  8. 8.
    H. L. Liu, T. H. Kao and B. H. Chen, Chromatographia, 60, 411 (2004).CrossRefGoogle Scholar
  9. 9.
    G. W. Lee and S. Y. Byun, Biotechnol. Bioprocess Eng., 8, 299 (2003).CrossRefGoogle Scholar
  10. 10.
    A. M. Sieuwerts, J. G. M. Klijkn, H. A. Peters and J. A. Foekens, Eur. J. Clin. Chem. Clin. Biochem., 33, 813 (1995).Google Scholar
  11. 11.
    J. Hosoi, E. Abe, T. Suda and T. Kuroki, Cancer Res., 45, 1474 (1985).Google Scholar
  12. 12.
    M. M. Bradford, Anal. Biochemistry, 72, 248 (1976).CrossRefGoogle Scholar
  13. 13.
    A. Görg, C. Obermaier, G. Boguth, A. Harder, B. Scheibe, R. Wildgruber and W. Weiss, Electrophoresis, 21, 1037 (2000).CrossRefGoogle Scholar
  14. 14.
    G. W. Lee, H. C. Yoon and S. Y. Byun, Enzyme and Microbial Technology, 35, 632 (2004).CrossRefGoogle Scholar
  15. 15.
    B. B. Fuller, D. T. Spaulding and D. R. Smith, Exp. Cell Res., 262, 197 (2001).CrossRefGoogle Scholar
  16. 16.
    N. Puri, J. M. Gardner and M. H. Brilliant, J. Invest. Dermatol., 115, 607 (2000).CrossRefGoogle Scholar
  17. 17.
    K. Kameyama, T. Takemura, Y. Hamada, C. Sakai, S. Kondoh, S. Nishiyama, K. Urabe and V. J. Hearing, J. Invest. Dermatol., 100, 126 (1993).CrossRefGoogle Scholar
  18. 18.
    D. Graphodatskaya, H. Joerg and G. Stranzinger, J. Recept. Signal Transduct Res., 22, 421 (2002).CrossRefGoogle Scholar
  19. 19.
    S. De Schepper, J. M. Boucneau, W. Westbroek, M. Mommaas, J. Onderwater, L. Messiaen, J. M. Naeyaert and J. L. Lambert, J. Invest. Dermatol., 126, 635 (2006).CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Hye In Lee
    • 1
  • Byoung Sam Yoo
    • 2
  • Mi Ae Yoo
    • 3
  • Sang Yo Byun
    • 1
    Email author
  1. 1.Department of Molecular Science & TechnologyAjou UniversitySuwon, KyunggiKorea
  2. 2.Cosmetic R&D CenterCOSMAX Inc.Hwa Sung, KyunggiKorea
  3. 3.Department of Applied BiotechnologyAjou UniversitySuwon, KyunggiKorea

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