Inhibition of melanogenesis and melanin transportation by Gynostemma pentaphyllum
- 126 Downloads
The extract of Gynostemma pentaphyllum was tested to control the melanogenesis in B16 melanoma. Cytotoxic effect by the extract was observed when the dose concentration was higher than 2 mg/L. Most of the inhibitory effect was obtained by the reduced accumulation of extra-cellular melanin. When the extract was dosed as 2 mg/L, the extra-cellular melanin produced was only 24% of the control. Proteome analysis with 2-D PAGE showed that various proteins involved in melanogenesis were down-regulated by Gynostemma pentaphyllum. In addition to other proteins related to the intra-cellular melanogenesis, Rab-27b and Rab-38 could explain the remarkable decrease in extra-cellular melanin accumulation by reduced melanin transfer to keratinocyte.
Key wordsGynostemma pentaphyllum Melanogenesis Inhibition 2-D PAGE
Unable to display preview. Download preview PDF.
- 5.A. J. Sober and T. B. Fitzpatrick, Prog. Chem. Org. Natural Products, 31, 521 (1974).Google Scholar
- 6.B. Karine, L. Elizabeth, L. F. James, G. S. Yiqun, A. Gretchen, P. Kirsi, D. E. Allen, F. Kenji, A. N. David, G. A. Natalie and A. R. Katheryn, Cancer research, 63, 6716 (2003).Google Scholar
- 10.A. M. Sieuwerts, J. G. M. Klijkn, H. A. Peters and J. A. Foekens, Eur. J. Clin. Chem. Clin. Biochem., 33, 813 (1995).Google Scholar
- 11.J. Hosoi, E. Abe, T. Suda and T. Kuroki, Cancer Res., 45, 1474 (1985).Google Scholar