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Expression of coxsackievirus and adenovirus receptor in human lung cancer: Possible clinical significance

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Clinical Oncology and Cancer Research

Abstract

Objective

To explore the relationship between CAR and the development of human lung cancer, as well as to provide the basis for the clinical treatment of lung cancer using an adenovirus vector-based gene therapy.

Methods

CAR expression was assessed immunohistochemically in tumoral, paraneoplastic and normal samples from 112 lung cancer patients. At the same time, the mRNA and protein expression of CAR in 32 cases were determined by RT-PCR and Western blot. The relationship between CAR expression and clinicopathologic parameters was statistically analyzed.

Results

There was no expression of CAR in normal lung tissue but a little in paraneoplastic tissue. The positive rate was 43% in squamous cell carcinoma, and 70% in adenocarcinoma. Both were much significantly higher than that in paraneoplastic tissue. The CAR expression level in adenocarcinoma was higher than that in squamous cell cancer. mRNA expression by RT-PCR and protein expression by Western blot were consistent with immunohistochemistry results.

Conclusion

CAR is overexpressed in human lung cancer, especially in adenocarcinoma. This data offer the reliable basis for adenovirus-mediated gene therapy of lung cancer; more important, CAR may take part in the formation or development of lung cancer; this may be exploitable for the development of antibody-directed therapy in human lung cancer.

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Authors and Affiliations

  1. Department of Pathology, Tianjin Cancer Institution and Hospital, Tianjin Medical University, Tianjin, 300060, China

    Lei-na Sun, An-kang Gu, Zhong-li Zhan, Qian Wang & Bao-cun Sun

  2. Environment and Health Medical Division, Military Medical Scientific Institutions, Tianjin, 300050, China

    Zhao-li Chen & Jun-wen Li

Authors
  1. Lei-na Sun
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  2. An-kang Gu
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  3. Zhao-li Chen
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  4. Zhong-li Zhan
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  5. Qian Wang
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  6. Jun-wen Li
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  7. Bao-cun Sun
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Correspondence to Bao-cun Sun.

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Sun, Ln., Gu, Ak., Chen, Zl. et al. Expression of coxsackievirus and adenovirus receptor in human lung cancer: Possible clinical significance. Clin. Oncol. Cancer Res. 7, 48–54 (2010). https://doi.org/10.1007/s11805-010-0048-4

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  • DOI: https://doi.org/10.1007/s11805-010-0048-4

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