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P2X7 receptor mediated growth-inhibitory effect in KG1a cell line

  • Published:
Chinese Journal of Clinical Oncology

Abstract

Objective

This study was conducted to investigate ATP-induced growth inhibition in human leukemic cells KG1a.

Methods

ATP inhibited cell growth was analyzed by MTS assay. Externalization of phosphatidylserine could be detected by Annexin-V-FITC apoptosis staining a er activation of the P2X7 receptor. P2X7 mediated pore formation was detected in KG1a cells by Yo-Pro-1 uptake assay.

Results

ATP inhibited cell growth in a dose-dependent manner. The cytotoxic effect could be blocked by P2X7 antagonists, oxidized ATP (oATP) and KN62. Externalization of phosphatidylserine could be detected in a time-dependent manner. P2X7 mediated pore formation could be detected in KG1a cells. These effects could not be observed in P2X7 null Ramos cells.

Conclusion

The results and our previously reports that mRNA, protein expression and calcium response of the P2X7 receptor in KG1a cells, suggested that extracellular ATP effectively induces growth inhibition through apoptosis in KG1a cells by activation of P2X7 receptor, and that may be mediated by extracellular Ca2+ influx and pore formation.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, North China Coal Medical College, Tangshan, 063000, Heibei Province, China

    Xiujun Zhang

  2. Department of Environment & Chemical Engineering, Tangshan College, Tangshan, 063000, Heibei Province, China

    Lijun Meng

Authors
  1. Xiujun Zhang
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  2. Lijun Meng
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Corresponding author

Correspondence to Xiujun Zhang.

Additional information

This work was supported by a grant from the National Natural Science Foundation of China (No.30671092).

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Zhang, X., Meng, L. P2X7 receptor mediated growth-inhibitory effect in KG1a cell line. Chin. J. Clin. Oncol. 5, 400–406 (2008). https://doi.org/10.1007/s11805-008-0400-0

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  • DOI: https://doi.org/10.1007/s11805-008-0400-0

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