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Identification of tumor progression-related genes in astrocytoma cell lines

  • Published:
Chinese Journal of Clinical Oncology

Abstract

Objective

To identify progression-related genes that lead to astrocytoma progression from a low to a high grade by analyzing the gene expression profi1es from different tumor grades, and all-trans retinoic acid-treated astrocytoma cell lines.

Methods

In this study, all-trans retinoic acid (ATRA) was used to induce differentiation of SHG-44 cells. Then, by using a cDNA microarray, we compared gene expression profiling in different grades of astrocytoma cell lines (CHG-5, WHO Grade II and SHG-44, WHO Grade IV) and in ATRA- induced differentiation in SHG-44 cells. A panel of overlapped genes that might be related to tumor progression was identified, and the cell line individual variation was avoided as well.

Results

In the 31 overlapped genes, the stable over-expression of MDM2 and UGB as well as the repression of SOD2, G3BP, and CSTB in parental SHG-44 cells was observed and their possible roles in tumor progression were discussed. Moreover, validation of some differentially expressed genes was confirmed by Northern blots.

Conclusion

The overlapped genes reported in this study might relate to progression of astrocytoma. Further study of progression-related genes may be helpful to explore the genetic pathways that are involved in astrocytoma progress from a low to a high grade.

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Authors and Affiliations

  1. Department of Neurosurgery, People’s Hospital of Deyang City, Deyang, 618000, Sichuan Province, China

    Yi Zeng & Yangyun Han

  2. Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China

    Yi Zeng & Chao You

  3. Department of Neurobiology, Third Military Medical University, Chongqing, 400038, China

    Zhong Yang

Authors
  1. Yi Zeng
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  2. Zhong Yang
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  3. Chao You
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  4. Yangyun Han
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Corresponding author

Correspondence to Zhong Yang.

Additional information

This work was supported partly by a grant from the Sichuan Provincial Bureau of Health, China (No.050209).

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Zeng, Y., Yang, Z., You, C. et al. Identification of tumor progression-related genes in astrocytoma cell lines. Chin. J. Clin. Oncol. 5, 391–399 (2008). https://doi.org/10.1007/s11805-008-0391-x

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  • DOI: https://doi.org/10.1007/s11805-008-0391-x

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