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Phenylacetate down-regulates expression of RNA editing deaminase ADAR2 mRNA in U-251MG glioma cells

  • Original Articles
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Chinese Journal of Clinical Oncology

Abstract

OBJECTIVE To observe the effect of phenylacetate on the expression of RNA editing deaminase ADAR2 mRNA in glioma cells.

METHODS Primary glial cells from human brain tissue and glioma U-251MG cells were cultured. The expression of ADAR2 mRNA was detected by a semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The levels of ADAR2 mRNA expression before and after treatment with phenylacetate were tested by RT-PCR and image analysis. The level of ADAR2 gene expression was presented as the ratio expression rate (RER) of ADAR2 gene/β-actin based on computer image analysis.

RESULTS The ADAR2 mRNA displayed mild expression in brain glial cells, and a high expression level in high-grade malignant U-251MG glioma cells. Computer image analysis showed that the RERs of the ADAR2 gene in the U-251MG cells before and after treatment with 4.0, and 5.0 mM phenylacetate for 8 h were 100.0, 73.5, 60.3, respectively. The expression of ADAR2 mRNA was decreased by phenylacetate in glioma U-251MG cells.

CONCLUSION Phenylacetate can decrease the expression of ADAR2 mRNA in glioma cells, suggesting that phenylacetate, as a drug, may act on the course of RNA editing in gliomas.

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Authors and Affiliations

  1. Department of Neurosurgery, Sino-Japanese Friendship Hospital, Jilin University, 130033, Changchun, China

    Yu Tian, Yufei Gao & Xingli Zhao

  2. Department of Pathophysiology, School of Basic Medical Sciences, Jilin University, 130021, Changchun, China

    Yuzuo Pan

  3. Department of Neurosurgery, Peking Union Medical College Hospital, Peking Union Medical College, 100005, Beijing, China

    Guilin Li, Jun Gao & Renzhi Wang

  4. Key Laboratory for Molecular Enzymology & Engineering, the Ministry of Education, Jilin University, 130021, Changchun, China

    Yu Tian & Guiying Li

Authors
  1. Yu Tian
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  2. Yuzuo Pan
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  3. Yufei Gao
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  4. Guilin Li
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  5. Jun Gao
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  6. Xingli Zhao
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  7. Guiying Li
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  8. Renzhi Wang
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Corresponding author

Correspondence to Yu Tian.

Additional information

This study was supported by the National Nature Science Foundation of China (No. 30271334).

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Tian, Y., Pan, Y., Gao, Y. et al. Phenylacetate down-regulates expression of RNA editing deaminase ADAR2 mRNA in U-251MG glioma cells. Chin. J. Clin. Oncol. 3, 1–4 (2006). https://doi.org/10.1007/s11805-006-0062-8

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  • DOI: https://doi.org/10.1007/s11805-006-0062-8

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